LOCUS       sequence-239323-       12986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows fluorescent (mVENUS; mCherry) co-localization of two proteins
            in Agrobacterium- transformed plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12986)
  TITLE     Feher Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDOE20 mC VN
FEATURES             Location/Qualifiers
     source          1..12986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    215..239
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(325..577)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        complement(1149..1529)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2203..2910
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MMSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2616..2635
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2943..3650
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    4342..4396
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(4398..4400,4401..4403,4404..5114)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4443..4464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4704..4723)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5051..5072
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5200..5452
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5503..5520)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5503..5519)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5512..5534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5722..5746
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7047..7676
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8105..9178
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9244..9438
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9674..9696)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9782..9922
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9837..9856
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9937..9954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10108..10696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10188..10207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11982..12776)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
       61 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
      121 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
      181 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
      241 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg
      301 ccgaattaat tcgagctcgg atctgataat ttatttgaaa attcataaga aaagcaaacg
      361 ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt aggatattgg
      421 ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat gtcttcaatt
      481 cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg atcattcatt
      541 gtttgtttgg ttgcctttgc caacatggga gtccaagatt ctgcagtcaa atctcggtga
      601 cgggcaggac cggacggggc ggtacctcac tctgactcct tctcgaaggt ttgagtacct
      661 tcaggacatc ctcttgaaac attagattca acttcaattg gagccaactg caaaagttct
      721 tgcttagacc taattgccat ctcacacaaa tgttgaagag ttcttgatcc tccagaaaca
      781 gtgatactaa ctcccctgaa cctaatagaa taagtacatc cgatttgatc gaacccaaag
      841 aatcttgatg cagcatagtt gactgaatct ccagtccaag atccaagcac tctatgcaat
      901 gatgcttcag ttctatcgta tctgagatac ctcttaaaca caacttttcc gaaaccccaa
      961 gattccttga aaccaagtgg attatcttga tttgagttcg tctcatcgtt atgcaatctc
     1021 cactcagtcc aagatggact ttcatcagga agtttgaaag gagaagttgt acctcctgat
     1081 cctccatccc atctttcaga gttagcttgt tctctagcat catttccttg tatagctcgt
     1141 tccattcgcg atttggtgta tcgagattgg ttatgaaatt cagatgctag tgtaatgtat
     1201 tggtaatttg ggaagatata ataggaagca aggctattta tccatttctg aaaaggcgaa
     1261 atggcgtcac cgcgagcgtc acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca
     1321 cttttgacta gcgaggcttg gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg
     1381 gatcaagaaa aagttggaat agaaacagaa tacccgcgaa attcaggccc ggttgccatg
     1441 tcctacacgc cgaaataaac gaccaaatta gtagaaaaat aaaaactgac tcggatactt
     1501 acgtcacgtc ttgcgcactg atttgaaaaa tctcagaatt cttgtcgacg agtcagtaat
     1561 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac
     1621 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac
     1681 aacgctcaat acacctgtca ttttattatt agctattgct tcaccgcctt agctttctcg
     1741 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc
     1801 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc
     1861 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt
     1921 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga
     1981 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag
     2041 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg
     2101 atttctaact agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct
     2161 gagtttttct gattaacagg gagaggacac gctcgagcca ccatgatgag caagggcgag
     2221 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     2281 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     2341 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     2401 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     2461 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     2521 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     2581 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     2641 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2701 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     2761 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2821 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2881 tccaccggcg gcatggacga gctgtacaag tctagacccg ggggttaatt aaccctagag
     2941 tcctgcttta atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg
     3001 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     3061 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     3121 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     3181 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     3241 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     3301 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     3361 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     3421 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     3481 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     3541 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     3601 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gacctgctcc
     3661 aagagtatgg ttgtcgacga gtcagtaata aacggcgtca aagtggttgc agccggcaca
     3721 cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa aaataaggca
     3781 attagccaaa aacaactttg cgtgtaaaca acgctcaata cacctgtcat tttattatta
     3841 gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt ttcttcttct
     3901 tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt tcaatttctc
     3961 aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat ttctgtgttc
     4021 cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt atatgttctt
     4081 tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat cgttagatat
     4141 catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa taatttgagt
     4201 tttgtcgaat aattactctt cgatttgtga tttctaacta gatctggtgt tagtttctag
     4261 tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacaggg acacgcctca
     4321 ggtacagagc atatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa
     4381 ttacatttac aattacaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4441 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4501 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc
     4561 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct
     4621 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4681 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4741 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4801 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg
     4861 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     4921 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4981 tgctgcccga caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga
     5041 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5101 acgagctgta caagtcaggt gcaggttctg gtggaggtag cgggatgggg tccctacgta
     5161 gtcacgtgac gtccggatga gcgatcgcca tttaaatagg atcgttcaaa catttggcaa
     5221 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     5281 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     5341 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     5401 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgttaacga
     5461 tatgtcgagg ctcagcagga cctgcaggca tgcaagcttg gcactggccg tcgttttaca
     5521 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     5581 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     5641 cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct
     5701 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     5761 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     5821 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     5881 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     5941 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     6001 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     6061 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     6121 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     6181 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     6241 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     6301 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     6361 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     6421 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     6481 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     6541 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     6601 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     6661 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     6721 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     6781 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     6841 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     6901 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     6961 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     7021 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     7081 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     7141 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     7201 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     7261 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     7321 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     7381 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     7441 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     7501 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     7561 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     7621 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     7681 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     7741 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     7801 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     7861 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     7921 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     7981 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     8041 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     8101 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     8161 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     8221 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     8281 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     8341 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     8401 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     8461 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     8521 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     8581 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     8641 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     8701 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     8761 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     8821 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     8881 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     8941 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     9001 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     9061 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     9121 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     9181 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     9241 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     9301 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     9361 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     9421 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     9481 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     9541 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     9601 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     9661 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9721 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9781 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     9841 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     9901 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     9961 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    10561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10681 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    10741 cgaaaactca cgttaaggga ttttggtcat gcattctagg gaaggtgcga acaagtccct
    10801 gatatgagat catgtttgtc atctggagcc atagaacagg gttcatcatg agtcatcaac
    10861 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
    10921 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
    10981 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
    11041 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
    11101 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgccggac cgcaccacca
    11161 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
    11221 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
    11281 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
    11341 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
    11401 ccaagagtgg cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc
    11461 agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
    11521 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
    11581 ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg
    11641 aatacatgaa agccagcatc cgggccaggg tggagcaccc atttcgcatc atcaagcgac
    11701 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
    11761 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
    11821 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
    11881 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
    11941 ccgagaagaa tttccccgct tattcgcacc ttccctaggt actaaaacaa ttcatccagt
    12001 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    12061 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    12121 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    12181 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    12241 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
    12301 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    12361 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    12421 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    12481 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    12541 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    12601 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    12661 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    12721 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    12781 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    12841 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
    12901 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
    12961 ggtgatcaca ggcagcaacg ctctgt
//
