LOCUS       sequence-239329-        9360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9360)
  TITLE     HDAC4 Plasmids
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HDAC4-FLAG
FEATURES             Location/Qualifiers
     source          1..9360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..51)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        223..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        603..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     851..870
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        851..869
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          906..1478
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             4851..4874
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4901..4919)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4902..4919)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4939..4956)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4945..5169
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5215..5643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5302..5321)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5512..5533
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5652..5672)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5657..5986
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5837..5972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5899..5918
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6053..6847
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6107..6126)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6717..6736
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7021..7142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7058..7077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7112..7131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7191..7207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7191..7207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7204..7226)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7215..7231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7239..7245,7246..7263,7264..7269))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7284..7305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7422..7439)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7593..8181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7673..7692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8352..9143,9144..9212))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8975..8994
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9213..9317)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
      841 ttatcgaaat taatacgact cactataggg agacccaagc tagagtcgat cctgagaact
      901 tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt
      961 tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc
     1021 accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt
     1081 ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt
     1141 aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac
     1201 ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa
     1261 ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa
     1321 atattcttat tggtagaaac aactacatcc tggtcatcat cctgcctttc tctttatggt
     1381 tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct
     1441 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttt
     1501 gtgctgtcga ccccaagctt ggcccgggcg gatccactag taacggccgc cagtgtgctg
     1561 gaattctgca gatatccatc acactggcgg ccgccatgag ctcccaaagc catccagatg
     1621 gactttctgg ccgagaccag ccagtggagc tgctgaatcc tgcccgcgtg aaccacatgc
     1681 ccagcacggt ggatgtggcc acggcgctgc ctctgcaagt ggccccctcg gcagtgccca
     1741 tggacctgcg cctggaccac cagttctcac tgcctgtggc agagccggcc ctgcgggagc
     1801 agcagctgca gcaggagctc ctggcgctca agcagaagca gcagatccag aggcagatcc
     1861 tcatcgctga gttccagagg cagcacgagc agctctcccg gcagcacgag gcgcagctcc
     1921 acgagcacat caagcaacaa caggagatgc tggccatgaa gcaccagcag gagctgctgg
     1981 aacaccagcg gaagctggag aggcaccgcc aggagcagga gctggagaag cagcaccggg
     2041 agcagaagct gcagcagctc aagaacaagg agaagggcaa agagagtgcc gtggccagca
     2101 cagaagtgaa gatgaagtta caagaatttg tcctcaataa aaagaaggcg ctggcccacc
     2161 ggaatctgaa ccactgcatt tccagcgacc ctcgctactg gtacgggaaa acgcagcaca
     2221 gttcccttga ccagagttct ccaccccaga gcggagtgtc gacctcctat aaccacccgg
     2281 tcctgggaat gtacgacgcc aaagatgact tccctcttag gaaaacagct tctgaaccga
     2341 atctgaaatt acggtccagg ctaaagcaga aagtggccga aagacggagc agccccctgt
     2401 tacgcaggaa agacgggcca gtggtcactg ctctaaaaaa gcgtccgttg gatgtcacag
     2461 actccgcgtg cagcagcgcc ccaggctccg gacccagctc acccaacaac agctccggga
     2521 gcgtcagcgc ggagaacggt atcgcgcccg ccgtccccag catcccggcg gagacgagtt
     2581 tggcgcacag acttgtggca cgagaaggct cggccgctcc acttcccctc tacacatcgc
     2641 catccttgcc caacatcacg ctgggcctgc ctgccaccgg cccctctgcg ggcacggcgg
     2701 gccagcagga caccgagaga ctcacccttc ccgccctcca gcagaggctc tcccttttcc
     2761 ccggcaccca cctcactccc tacctgagca cctcgccctt ggagcgggac ggaggggcag
     2821 cgcacagccc tcttctgcag cacatggtct tactggagca gccaccggca caagcacccc
     2881 tcgtcacagg cctgggagca ctgcccctcc acgcacagtc cttggttggt gcagaccggg
     2941 tgtccccctc catccacaag ctgcggcagc accgcccact ggggcggacc cagtcggccc
     3001 cgctgcccca gaacgcccag gctctgcagc acctggtcat ccagcagcag catcagcagt
     3061 ttctggagaa acacaagcag cagttccagc agcagcaact gcagatgaac aagatcatcc
     3121 ccaagccaag cgagccagcc cggcagccgg agagccaccc ggaggagacg gaggaggagc
     3181 tccgtgagca ccaggctctg ctggacgagc cctacctgga ccggctgccg gggcagaagg
     3241 aggcgcacgc acaggccggc gtgcaggtga agcaggagcc cattgagagc gatgaggaag
     3301 aggcagagcc cccacgggag gtggagccgg gccagcgcca gcccagtgag caggagctgc
     3361 tcttcagaca gcaagccctc ctgctggagc agcagcggat ccaccagctg aggaactacc
     3421 aggcgtccat ggaggccgcc ggcatccccg tgtccttcgg cggccacagg cctctgtccc
     3481 gggcgcagtc ctcacccgcg tctgccacct tccccgtgtc tgtgcaggag ccccccacca
     3541 agccgaggtt cacgacaggc ctcgtgtatg acacgctgat gctgaagcac cagtgcacct
     3601 gcgggagtag cagcagccac cccgagcacg ccgggaggat ccagagcatc tggtcccgcc
     3661 tgcaggagac gggcctccgg ggcaaatgcg agtgcatccg cggacgcaag gccaccctgg
     3721 aggagctaca gacggtgcac tcggaagccc acaccctcct gtatggcacg aaccccctca
     3781 accggcagaa actggacagt aagaaacttc taggctcgct cgcctccgtg ttcgtccggc
     3841 tcccttgcgg tggtgttggg gtggacagtg acaccatatg gaacgaggtg cactcggcgg
     3901 gggcagcccg cctggctgtg ggctgcgtgg tagagctggt cttcaaggtg gccacagggg
     3961 agctgaagaa tggctttgct gtggtccgcc cccctggaca ccatgcggag gagagcacgc
     4021 ccatgggctt ttgctacttc aactccgtgg ccgtggcagc caagcttctg cagcagaggt
     4081 tgagcgtgag caagatcctc atcgtggact gggacgtgca ccatggaaac gggacccagc
     4141 aggctttcta cagcgaccct agcgtcctgt acatgtccct ccaccgctac gacgatggga
     4201 acttcttccc aggcagcggg gctcctgatg aggtgggcac agggcccggc gtgggtttca
     4261 acgtcaacat ggctttcacc ggcggcctgg acccccccat gggagacgct gagtacttgg
     4321 cggccttcag aacggtggtc atgccgatcg ccagcgagtt tgccccggat gtggtgctgg
     4381 tgtcatcagg cttcgatgcc gtggagggcc accccacccc tcttgggggc tacaacctct
     4441 ccgccagatg cttcgggtac ctgacgaagc agctgatggg cctggctggc ggccggattg
     4501 tcctggccct cgagggaggc cacgacctga ccgccatttg cgacgcctcg gaagcatgtg
     4561 tttctgcctt gctgggaaac gagcttgatc ctctcccaga aaaggtttta cagcaaagac
     4621 ccaatgcaaa cgctgtccgt tccatggaga aagtcatgga gatccacagc aagtactggc
     4681 gctgcctgca gcgcacaacc tccacagcgg ggcgttctct gatcgaggct cagacttgcg
     4741 agaacgaaga agccgagacg gtcaccgcca tggcctcgct gtccgtgggc gtgaagcccg
     4801 ccgaaaagag accagatgag gagcccatgg aagaggagcc gcccctgggg gattataaag
     4861 atgatgatga taaataagaa ttcgatctag agggccctat tctatagtgt cacctaaatg
     4921 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4981 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5041 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5101 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5161 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5221 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5281 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5341 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5401 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5461 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5521 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5581 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5641 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5701 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5761 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5821 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5881 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     5941 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6001 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6061 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6121 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6181 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6241 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6301 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6361 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6421 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6481 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6541 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6601 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6661 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6721 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6781 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6841 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6901 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6961 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7021 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7081 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7141 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7201 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7501 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7561 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7621 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7681 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7741 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7801 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7861 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7921 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7981 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8041 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8101 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8161 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8221 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8281 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8341 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9181 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9241 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9301 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
//
