LOCUS       sequence-239346-        7666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, 
            Brugge JS, Swat W
  TITLE     Vav family proteins couple to diverse cell surface receptors.
  JOURNAL   Mol Cell Biol. 2000 Sep . 20(17):6364-73.
  PUBMED    10938113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Sep . 20(17):6364-73."
COMMENT     Sequence Label: pC.HA Vav2
FEATURES             Location/Qualifiers
     source          1..7666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             3307..3333
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          3365..3937
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4069..4088)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4134..4189
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4188..4207)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4543..4678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4784..4801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4955..5543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5035..5054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5714..6505,6506..6574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6337..6356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6575..6679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6747..6765
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6803..6825)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6925..6944
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7011..7466)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7148..7169)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7360..7379
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7592..7614
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7606..7623
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7607..7623
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7630..7649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7630..7648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
      661 tctagaatgg agcagtggcg acagtgcggc cgctggctca tcgattgcaa ggtcctgccg
      721 cccaaccacc gggtggtgtg gccctcggcc gtggtcttcg acctggcgca ggcgctgcgc
      781 gacggggtcc ttctgtgcca gctgctgcac aacctctccc ccggctccat cgacctcaag
      841 gacatcaact tccggccgca gatgtcccag tttctgtgtt tgaagaacat acgcaccttc
      901 ctgaaagtct gccacgataa atttggatta aggaacagcg agctgtttga cccctttgac
      961 ctcttcgatg tgcgagactt tggaaaggtc atctccgcgg tgtcgaggct ctccctgcac
     1021 agcatcgcgc agaacaaagg gatcaggcct tttccctcag aggagaccac agagaatgac
     1081 gatgacgtct accgcagcct ggaggagctg gccgacgagc atgacctggg ggaggacatc
     1141 tacgactgcg tcccgtgtga ggatggaggg gacgacatct acgaggacat catcaaggtg
     1201 gaggtgcagc agcccatgat tagatacatg cagaaaatgg gcatgactga agatgacaag
     1261 aggaactgct gcctgctgga gatccaggag accgaggcca agtactaccg caccctggag
     1321 gacattgaga agaactacat gagccccctg cggctggtgc tgagcccggc ggacatggca
     1381 gctgtcttca ttaacctgga ggacctgatc aaggtgcatc acagcttcct gagggccatc
     1441 gacgtgtccg tgatggtggg gggcagcacg ctggccaagg tcttcctcga tttcaaggaa
     1501 aggctcctga tctacgggga gtactgcagc cacatggagc acgcccagaa cacactgaac
     1561 cagctcctgg ccagccggga ggacttcagg cagaaagtcg aggagtgcac actgaaggtc
     1621 caggatggaa aatttaagct gcaagacctg ctggtggtcc ccatgcagag ggtgctcaaa
     1681 taccacctgc tcttgaagga gcttctgagc cattctgcgg aacggcctga gaggcagcag
     1741 ctcaaagaag cactggaagc catgcaggac ttggcgatgt acatcaatga agttaaacgg
     1801 gacaaggaga ccttgaggaa aatcagcgaa tttcagagtt ctatagaaaa tttgcaagtg
     1861 aaactggagg aatttggaag accaaagatt gacggggaac tgaaagtccg gtccatagtc
     1921 aaccacacca agcaggacag gtacttgttc ctgtttgaca aggtggtcat cgtctgcaag
     1981 cggaagggct acagctacga gctcaaggag atcatcgagc tgctgttcca caagatgacc
     2041 gacgacccca tgaacaacaa ggacgtcaag aagtctcacg ggaaaatgtg gtcctacggc
     2101 ttctacctaa ttcaccttca aggaaagcag ggcttccagt ttttctgcaa aacagaagat
     2161 atgaagagga agtggatgga gcagtttgag atggccatgt caaacatcaa gccagacaaa
     2221 gccaatgcca accaccacag tttccagatg tacacgtttg acaagaccac caactgcaaa
     2281 gcctgcaaaa tgttcctcag gggcaccttc taccagggat acatgtgtac caagtgtggc
     2341 gtcggggcac acaaggagtg cctggaagtg atacctccct gcaagttcac ttctcctgca
     2401 gatctggacg cctccggagc gggaccaggt cccaagatgg tggccgtgca gaattaccat
     2461 ggcaacccag cccctcccgg gaagcctgtg ctgaccttcc agaccggcga cgtgcttgag
     2521 ctgctgaggg gcgaccctga gtctccgtgg tgggagggtc gtctggtaca aaccaggaag
     2581 tcagggtatt tccccagctc atctgtgaag ccctgccctg tggatggaag gccgcccatc
     2641 agccggccgc catcccggga gatcgactac actgcatacc cctggtttgc aggtaacatg
     2701 gagaggcagc agacggacaa cctgctcaag tcccacgcca gtgggaccta cctgatcagg
     2761 gagcggcctg ccgaggctga gcgctttgca ataagcatca agttcaatga tgaggtgaag
     2821 cacatcaagg tggtggagaa ggacaactgg atccacatca cagaggccaa gaaattcgac
     2881 agcctcctgg agttggtgga gtactaccag tgccactcac tgaaggagag cttcaagcag
     2941 ctggacacca cactcaagta cccctacaag tcccgggaac gttcggcctc cagagcctcc
     3001 agccggtccc cagcttcctg tgcttcctac aacttttctt ttctcagtcc tcagggcctc
     3061 agctttgctt ctcagggccc ctccgctccc ttctggtcag tgttcacgcc ccgcgtcatc
     3121 ggcacagctg tggccaggta taactttgcc gcccgagata tgagggagct ttcgctgcgg
     3181 gagggtgacg tggtgaggat ctacagccgc attggcggag accagggctg gtggaagggc
     3241 gagaccaacg gacggattgg ctggtttcct tcaacgtacg tagaagagga gggcatccag
     3301 actagttatc cgtacgacgt accagactac gcataagaaa agtgaggatc ctgagaactt
     3361 cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
     3421 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
     3481 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
     3541 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
     3601 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
     3661 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
     3721 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
     3781 tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt
     3841 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
     3901 tgctaaccat gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt
     3961 tgtgctgtct catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa
     4021 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     4081 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     4141 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     4201 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     4261 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     4321 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     4381 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     4441 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     4501 tctcttatgg agatccctcg aggagctttt tgcaaaagcc taggcctcca aaaaagcctc
     4561 ttcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     4621 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     4681 gcggagttag gggcgggact atggttgctg actaattgct gcattaatga atcggccaac
     4741 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6721 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     6781 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6841 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6901 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6961 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
     7021 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     7081 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7141 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     7201 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7261 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7321 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7381 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7441 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
     7501 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     7561 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     7621 agtgaattgt aatacgactc actatagggc gaattcgagc tcgccc
//
