LOCUS       sequence-239349-        8317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of GFP C-MYC-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, 
            Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, 
            Pelechano V, Cittaro D, Tonon G
  TITLE     Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the 
            decapping complex to the nucleolus.
  JOURNAL   EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 
            10.15252/embj.201899179. Epub 2018 Oct 29.
  PUBMED    30373810
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-12-3"; volume: "37(23). pii: embj.201899179. doi"; 
            pages: " 10.15252/embj.201899179. Epub 2018 Oct 29"
COMMENT     Sequence Label: pLenti c-MYC-DDK-Puro GFP
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        140..158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(271..273,274..276,277..987)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(271..273,274..276,277..987)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(316..337)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(577..596)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     924..945
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             991..1020
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1039..1062
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1096..1669
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1119..1671
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1263..1280)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1490..1509
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1671..2270
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1671..1690)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2167..2187
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(2300..2333)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2389..2977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2442..2462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2860..2871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2980..2996)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2981..2997)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3506..3686
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3748..4336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3828..3847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4413..5072)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4987..5006
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5073..5177)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5252..5271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5442..5821
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5823..6021
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6039..6219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6266..6391
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6888..7121
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7306..7350
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7648..7765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7848..8227
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8228..8317,1..114)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
       61 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      121 gtgaaccgtc agaattttgt aatacgactc actatagggc ggccgggaat tcgtcgactg
      181 gatccggtac cgaggagatc tgccgccgcg atcgccggcg cgccagatct caagcttaac
      241 tagctagcgg accgacgcgt acgcggccgc atggtgagca agggcgagga gctgttcacc
      301 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      361 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      421 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      481 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      541 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      601 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      661 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      721 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      781 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      841 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
      901 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      961 actctcggca tggacgagct gtacaagctc gagcagaaac tcatctcaga agaggatctg
     1021 gcagcaaatg atatcctgga ttacaaggat gacgacgata aggtttaaac ctaggcgtag
     1081 cggccgcaaa ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     1141 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     1201 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     1261 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     1321 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     1381 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     1441 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     1501 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     1561 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     1621 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atgaccgagt
     1681 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggcagta cgcaccctcg
     1741 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccagac cgccacatcg
     1801 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     1861 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     1921 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     1981 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     2041 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     2101 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     2161 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     2221 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tgtacaagta
     2281 ggattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
     2341 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
     2401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2761 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     2881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     2941 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg
     3001 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     3061 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     3121 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3181 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     3241 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     3301 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     3361 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     3421 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     3481 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     3541 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3601 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3661 ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa
     3721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4201 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4261 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4321 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4381 cgaaaactca cgttaaggga ttttggtcat gattacgccc cgccctgcca ctcatcgcag
     4441 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     4501 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     4561 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     4621 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     4681 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     4741 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     4801 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaactcc
     4861 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     4921 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     4981 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     5041 acggtggtat atccagtgat ttttttctcc atactcttcc tttttcaata ttattgaagc
     5101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     5221 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5281 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     5341 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     5401 gttttgcgct gcttcgcgat gtacgggcca gatatcgcgt tgacattgat tattgactag
     5461 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     5521 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     5581 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     5641 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     5701 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     5761 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     5821 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     5881 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     5941 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     6001 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     6061 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6121 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6181 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     6241 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     6301 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     6361 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     6421 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     6481 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     6541 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     6601 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     6661 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     6721 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     6781 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     6841 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     6901 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     6961 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     7021 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     7081 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     7141 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     7201 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     7261 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     7321 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     7381 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     7441 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     7501 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     7561 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     7621 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     7681 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     7741 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     7801 gttagtaccg ggcccgctct agacatgtcc aatatgaccg ccatgttgac attgattatt
     7861 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     7921 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7981 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8041 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8101 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     8161 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     8221 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     8281 gggatttcca agtctccacc ccattgacgt caatggg
//
