LOCUS       sequence-239375-        4597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p300 core domain with GreenGate D-E flanking sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Lee JE, Neumann M, Duro DI, Schmid M
  TITLE     CRISPR-based tools for targeted transcriptional and epigenetic 
            regulation in plants.
  JOURNAL   PLoS One. 2019 Sep 26;14(9):e0222778. doi: 
            10.1371/journal.pone.0222778. eCollection 2019.
  PUBMED    31557222
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-09-26"; volume: "14(9):e0222778. doi"; pages: " 
            10.1371/journal.pone.0222778. eCollection 2019"
COMMENT     Sequence Label: pJL023
FEATURES             Location/Qualifiers
     source          1..4597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     189..211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     203..220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     204..220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2201..2217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2201..2217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2214..2236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2225..2241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2249..2255,2256..2273,2274..2279))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2294..2315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2432..2449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2603..3191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2683..2702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3362..4153,4154..4222))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3985..4004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4223..4327)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4395..4413
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4451..4473)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4573..4592
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
       61 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
      121 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
      181 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacctc
      241 gcgaatgcat ctagatatcg gatcccgacg gtctcatcag gtatttttaa gccagaggag
      301 cttagacaag cacttatgcc tactcttgag gctctttaca gacaagatcc tgaatctctt
      361 ccattcagac agcctgtgga ccctcagctt cttggtattc ctgactactt cgatattgtg
      421 aagtctccta tggatctttc tacaattaaa agaaaacttg acacaggaca ataccaagaa
      481 ccttggcaat acgttgatga catctggctt atgtttaaca atgcttggct ttacaacaga
      541 aagacttcta gagtttacaa atattgctct aaactttctg aagtgttcga acaagaaatt
      601 gatccagtga tgcaatctct tggttactgt tgcggtagaa aacttgagtt ctctcctcaa
      661 actctttgct gctatggaaa gcaactttgt actattccta gagatgcaac atactattct
      721 tatcaaaaca gataccattt ctgtgaaaaa tgctttaacg aaatccaggg tgaatctgtt
      781 tctcttggag acgacccttc tcagcctcag acaacaatca ataaagagca gttctctaag
      841 agaaagaacg atactcttga ccctgagctt ttcgtggagt gtactgagtg cggtagaaag
      901 atgcatcaga tttgtgttct tcatcacgag atcatttggc cagctggttt tgtttgcgat
      961 ggttgtctta agaagtctgc aagaactaga aaagagaata agttctctgc aaagagactt
     1021 ccttctacta gacttggaac tttccttgaa aacagagtga acgatttcct tagaagacag
     1081 aatcatcctg agtctggaga ggtgacagtg agagtggttc atgcatctga taaaactgtt
     1141 gaggttaaac ctggaatgaa agctagattc gtggactctg gagagatggc agaatctttt
     1201 ccatacagaa caaaagctct ttttgcattc gaagagattg atggtgttga tctttgtttc
     1261 ttcggtatgc acgtgcaaga gtatggatct gactgccctc caccaaatca aagaagagtt
     1321 tacatttctt atcttgattc tgttcacttt tttagaccaa aatgccttag aacagcagtg
     1381 taccacgaga ttcttattgg ttatcttgag tatgtgaaaa aacttggtta cacaactgga
     1441 catatttggg cttgcccacc ttctgaaggt gatgattata tttttcattg ccaccctcca
     1501 gaccaaaaga ttcctaaacc taaaagactt caagagtggt acaaaaagat gcttgataaa
     1561 gcagtgtctg agagaattgt gcacgactac aaggacattt ttaaacaggc tacagaggat
     1621 agacttactt ctgcaaaaga gcttccttat tttgaaggag atttttggcc taatgttctt
     1681 gaggagtcta ttaaggaact tgagcaggag gaagaagaga gaaaaagaga agagaacaca
     1741 tctaatgagt ctacagacgt tactaaaggt gattctaaaa acgctaaaaa gaaaaacaat
     1801 aaaaaaactt ctaaaaataa atcttctctt tctagaggta acaaaaaaaa acctggaatg
     1861 ccaaacgttt ctaatgacct ttctcaaaaa ctttacgcaa caatggaaaa acataaagaa
     1921 gttttcttcg tgatcagact tattgctgga cctgctgcta attctcttcc tccaatcgtt
     1981 gacccagacc ctcttattcc ttgtgatctt atggatggaa gagacgcatt tcttactctt
     2041 gcaagagaca aacatcttga attttcttct cttagaagag cacagtggtc tacaatgtgt
     2101 atgcttgttg agcttcacac acaatctcaa gactagtaac tgcagagacc gacgggcccg
     2161 tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2221 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2281 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2341 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2401 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2761 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3001 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3301 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3361 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3421 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3481 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3541 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3601 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3661 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3721 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3781 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3841 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3901 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3961 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4021 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4081 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4141 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4201 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4261 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4321 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4381 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4441 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4501 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4561 ggcttaacta tgcggcatca gagcagattg tactgag
//
