LOCUS       sequence-239424-        8294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of PNRC1-W300A mutant N-RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, 
            Santoliquido BM, Frenquelli M, Segalla S, Bachi A, Doglioni C, 
            Pelechano V, Cittaro D, Tonon G
  TITLE     Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the 
            decapping complex to the nucleolus.
  JOURNAL   EMBO J. 2018 Dec 3;37(23). pii: embj.201899179. doi: 
            10.15252/embj.201899179. Epub 2018 Oct 29.
  PUBMED    30373810
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-12-3"; volume: "37(23). pii: embj.201899179. doi"; 
            pages: " 10.15252/embj.201899179. Epub 2018 Oct 29"
COMMENT     Sequence Label: pLenti4 Blast RFP-PNRC1 W300A
FEATURES             Location/Qualifiers
     source          1..8294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    510..528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             625..1317
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     protein_bind    complement(2336..2360)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2413..2454
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        2465..2512
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2531..2929
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3001..3234
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3306..3427
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3343..3362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3397..3416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3467..3602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3622..3641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3623..3641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3651..3668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3651..3667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3660..3682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3809..4264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3896..3915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4106..4127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4290..4394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4395..4463,4464..5255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4613..4632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5426..6014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5915..5934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6168..6185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6302..6323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6338..6343,6344..6361,6362..6368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6376..6392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6381..6403
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6400..6416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6400..6416
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6435..6455
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6437..6455
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6483..6709
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6710..6890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6937..7062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7555..7788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7973..8017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctccgcgg ccgcatgagc gagctgatca aggagaacat gcacatgaag
      661 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
      721 aagccctacg agggcaccca gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc
      781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaagcctt catcaaccac
      841 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
      901 atcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cttccagaac
      961 ggctgcatca tctacaacgt caagatcaac ggggtgaact tcccatccaa cggccctgtg
     1021 atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
     1081 ggcctgagag gccacagcca gatggccctg aagctcgtgg gcgggggcta cctgcactgc
     1141 tccttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcttc
     1201 cacttcgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     1261 cagcacgaga tggctgtggc caagtactgc gacctcccta gcaaactggg gcacagagcg
     1321 gccgccccct tcaccatgac tgtcgtctcc gtcccgcagc gggagccgct cgtcctgggt
     1381 ggccgccttg cgccgcttgg cttttcctcc cgaggttact ttggggccct cccgatggtg
     1441 accacggctc cgcctccttt accccggatc ccggaccccc gggcactgcc cccgaccctc
     1501 ttccttcctc atttcctagg gggagatggc ccgtgtctga ccccccagcc tcgcgctcca
     1561 gcagctctgc ccaaccgcag cctcgccgtg gcgggaggca ctcctcgggc agcgccgaag
     1621 aagcggcgaa agaagaaggt gcgggccagc cccgcagggc agctgcccag ccgcttccac
     1681 cagtaccagc agcaccggcc gagtctggag ggcggccgga gccccgcgac cggcccgagc
     1741 ggagcgcagg aggtcccggg cccggccgcc gccttggccc cgagtcctgc agccgcagcc
     1801 ggcacggagg gagccagccc cgaccttgcc ccgctgcggc ccgcggctcc cggccaaacc
     1861 cccctcagga aagaggtttt aaaatcaaag atgggaaaat cggagaaaat tgcccttccc
     1921 catggccagc ttgttcatgg tatacacttg tatgagcaac caaagataaa cagacagaaa
     1981 agcaaatata acttgccact aaccaagatc acctctgcaa aaagaaatga aaacaacttt
     2041 tggcaggatt ctgtttcatc tgacagaatt cagaagcagg aaaaaaagcc ttttaaaaat
     2101 accgagaaca ttaaaaattc gcatttgaag aaatcagcat ttctaactga agtgagccaa
     2161 aaggaaaatt atgctggggc aaagtttagt gatccacctt ctcctagtgt tcttccaaag
     2221 cctcctagtc acgcgatggg aagcactgtt gaaaattcca accaaaacag ggagctgatg
     2281 gcagtacact taaaaacgct cctcaaagtt caaacttaga agggtgggcg cgccgaccca
     2341 gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     2401 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     2461 ggttgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     2521 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     2581 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     2641 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     2701 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     2761 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     2821 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     2881 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag gtacctttaa
     2941 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3001 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3061 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     3241 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3301 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3421 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3601 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     3661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3781 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     3841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4261 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4921 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcagggggga
     6001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6421 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6481 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6541 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6601 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     6661 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     6721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     6901 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     6961 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7021 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7081 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7141 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7201 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7261 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7321 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7381 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8101 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8161 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8221 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     8281 agcttgggag ttcc
//
