LOCUS       sequence-239429-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  His-tagged TEV protease with solubility increasing mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   van den Berg S, Lofdahl PA, Hard T, Berglund H
  TITLE     Improved solubility of TEV protease by directed evolution.
  JOURNAL   J Biotechnol. 2006 Feb 10;121(3):291-8. doi: 
            10.1016/j.jbiotec.2005.08.006. Epub 2005 Sep 15.
  PUBMED    16150509
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2005.08.006"; journalName: "J Biotechnol"; date: 
            "2006-02-10- 10"; volume: "121"; issue: "3"; pages: "291-8"
COMMENT     Sequence Label: TEVSH
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             40..747
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSSICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKDTTTLQQHLVDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMNKPE
                     EPFQPVKEATQLMN"
     protein_bind    complement(767..791)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             817..858
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             868..885
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgttcat caacaagttt gtacaaaaaa gcaggctcgg gagaaagctt gtttaaggga
       61 ccacgtgatt ataacccgat atcgagctcc atttgtcatt tgacgaatga atctgatggg
      121 cacacaacat cgttgtatgg tattggattt ggtcccttca tcattacaaa caagcacttg
      181 tttagaagaa ataatggaac actgttggtc caatcactac atggtgtatt caaggtcaag
      241 gacaccacga ctttgcaaca acacctcgtt gatgggaggg acatgataat tattcgcatg
      301 cctaaggatt tcccaccatt tcctcaaaag ctgaaattta gagagccaca aagggaagag
      361 cgcatatgtc ttgtgacaac caacttccaa actaagagta tgtctagcat ggtgtcagac
      421 actagttgca cattcccttc atctgatggc atattctgga agcattggat tcaaaccaag
      481 gatgggcagt gtggcagtcc attagtatca actagagatg ggttcattgt tggtatacac
      541 tcagcatcga atttcaccaa cacaaacaat tatttcacaa gcgtgccgaa aaacttcatg
      601 gaattgttga caaatcagga ggcgcagcag tgggttagtg gttggcgatt aaatgctgac
      661 tcagtattgt gggggggcca taaagttttc atgaacaaac ctgaagagcc ttttcagcca
      721 gttaaggaag cgactcaact catgaatgaa ttggtgtact cgcaagaccc agctttcttg
      781 tacaaagtgg ttgatctgga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
      841 ctcggtctcg attctacgcg taccggtcat catcaccatc accattga
//
