LOCUS       sequence-239435-        2055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon plasmid for stable expression of lipid raft-targeted 
            Lyn-AktAR2 variant of FRET-based AKT activity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Havranek O, Xu J, Kohrer S, Wang Z, Becker L, Comer JM, Henderson J,
            Ma W, Man Chun Ma J, Westin JR, Ghosh D, Shinners N, Sun L, Yi AF, 
            Karri AR, Burger JA, Zal T, Davis RE
  TITLE     Tonic B-cell receptor signaling in diffuse large B-cell lymphoma.
  JOURNAL   Blood. 2017 Aug 24;130(8):995-1006. doi: 
            10.1182/blood-2016-10-747303. Epub 2017 Jun 23.
  PUBMED    28646116
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2016-10-747303"; journalName: "Blood"; date: 
            "2017-08-24- 24"; volume: "130"; issue: "8"; pages: "995-1006"
COMMENT     Sequence Label: pSBbi-Pur-Lyn-AktAR2
FEATURES             Location/Qualifiers
     source          1..2055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(49..51,52..54,55..765)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1318..2052
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 gccaccatgg ggtgcattaa gtctaagcgg aaggacaaaa aggatcccat ggtgagcaag
       61 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      241 ctgagctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aacgccatcc acggcaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      541 aacttcggcc tcaactgcaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagcgcat gcataagttt
      781 tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt
      841 cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag
      901 aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga
      961 ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac
     1021 atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta
     1081 ctgtctcaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat tttatctctg
     1141 gtcattttca taaacgacaa atttaagcag tgcctcgagc agaacaaagt tgatcgctct
     1201 gcaggtaagc caggcagcgg cgagggcagc accaagggcc tggtcgaccc tcgtccgcgc
     1261 tcgtgcacct ggccggaccc caggccggag tttggaggta ccggcggcag cgagctcatg
     1321 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1381 ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag
     1441 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1501 gacgagctgt acaagggagg taccggtgga tctatggtga gcaagggcga ggagctgttc
     1561 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1621 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
     1681 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctt
     1741 cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1801 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1861 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1921 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1981 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
     2041 cacaacatcg agtaa
//
