LOCUS       sequence-239451-        2373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon plasmid for stable expression of lipid raft-targeted 
            FRET-based kinase activity reporter with EV spacer, with AarI 
            cassette for cloning of a kinase-target peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2373)
  TITLE     Eric Davis SB transposon plasmids
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSBbi-Pur-LPAR
FEATURES             Location/Qualifiers
     source          1..2373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(49..51,52..54,55..765)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1636..2370
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 gccaccatgg ggtgcattaa gtctaagcgg aaggacaaaa aggatcccat ggtgagcaag
       61 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      241 ctgagctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aacgccatcc acggcaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      541 aacttcggcc tcaactgcaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagcgcat gcataagttt
      781 tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt
      841 cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag
      901 aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga
      961 ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac
     1021 atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta
     1081 ctgtctcaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat tttatctctg
     1141 gtcattttca taaacgacaa atttaagcag tgcctcgagc agaacaaagt tgatcgctct
     1201 gcaggtaagc caggcagcgg cgagggcagc accaagggcc tggtcgacgg aacctctgca
     1261 ggaggttccg cagggggttc cgccggagga agcgccggtg gctctgctgg cggctctgct
     1321 ggtgggtctg ccggcggatc tgcgggaggg agtgcgggtg gaagcgcagg aggcagtgcc
     1381 gggggatcag cggggggaag tgccgggggt agtgctggtg gctctgcagg aggttccgca
     1441 gggggttccg ccggaggaag cgccggtggc tctgctggcg gctctgctgg tgggtctgcc
     1501 ggcggatctg cgggagggag tgcgggtgga agcgcaggag gcagtgccgg gggatcagcg
     1561 ggaggaagtg ccgggggtag tgctggtggc agcggcgtcg acagtagcag gtgtgaatca
     1621 cctgctactg agctcatggg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     1681 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg
     1741 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1801 gggatcactc tcggcatgga cgagctgtac aagggaggta ccggtggatc tatggtgagc
     1861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1981 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2041 accctgggct acggccttca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     2101 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2281 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     2341 gccaacttca agatccgcca caacatcgag taa
//
