LOCUS       sequence-239455-        2388 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SB-transposon plasmid for stable expression of unphosphorylatable 
            ""dead"" version of lipid raft-targeted FRET-based PKA activity 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2388)
  TITLE     Eric Davis SB transposon plasmids
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSBbi-Puro_LPAR_AKAR-mut
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(49..51,52..54,55..765)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1651..2385
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
ORIGIN
        1 gccaccatgg ggtgcattaa gtctaagcgg aaggacaaaa aggatcccat ggtgagcaag
       61 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      241 ctgagctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aacgccatcc acggcaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      541 aacttcggcc tcaactgcaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagcgcat gcataagttt
      781 tctcaagaac agatcggcga aaacattgtg tgcagggtca tttgtaccac gggtcaaatt
      841 cccatccgag atttgtcagc tgatatttca caagtgctta aggaaaaacg atccataaag
      901 aaagtttgga catttggtag aaacccagcc tgtgactatc atttaggaaa catttcaaga
      961 ctgtcaaata agcatttcca aatactacta ggagaagacg gtaacctttt attgaatgac
     1021 atttccacta atgggacctg gttaaatggg caaaaagtcg agaagaacag caatcagtta
     1081 ctgtctcaag gtgatgaaat aaccgttggt gtaggcgtgg aatcagatat tttatctctg
     1141 gtcattttca taaacgacaa atttaagcag tgcctcgagc agaacaaagt tgatcgctct
     1201 gcaggtaagc caggcagcgg cgagggcagc accaagggcc tggtcgacgg aacctctgca
     1261 ggaggttccg cagggggttc cgccggagga agcgccggtg gctctgctgg cggctctgct
     1321 ggtgggtctg ccggcggatc tgcgggaggg agtgcgggtg gaagcgcagg aggcagtgcc
     1381 gggggatcag cggggggaag tgccgggggt agtgctggtg gctctgcagg aggttccgca
     1441 gggggttccg ccggaggaag cgccggtggc tctgctggcg gctctgctgg tgggtctgcc
     1501 ggcggatctg cgggagggag tgcgggtgga agcgcaggag gcagtgccgg gggatcagcg
     1561 ggaggaagtg ccgggggtag tgctggtggc agcggcgtcg acacaaaggg cctgcggaga
     1621 gctgtcctcg tggatggcgg aacagagctc atgggcggcg tgcagctcgc cgaccactac
     1681 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1741 taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1801 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aggtaccggt
     1861 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1921 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1981 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     2041 cccaccctcg tgaccaccct gggctacggc cttcagtgct tcgcccgcta ccccgaccac
     2101 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2161 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2221 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2281 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     2341 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgagtaa
//
