LOCUS       sequence-239457-        9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM
  TITLE     Reproducible doxycycline-inducible transgene expression at specific 
            loci generated by Cre-recombinase mediated cassette exchange.
  JOURNAL   Nucleic Acids Res. 2005  . 33(17):e147.
  PUBMED    16204450
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(17):e147."
COMMENT     Sequence Label: RV-L3-HyTK-2L
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        268..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        572..775
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    997..1354
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1419..1835
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1765..1787
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1803..1819
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2157..2173
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    2233..2266
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(join(2281..2994,2995..2997,2998..3000))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2324..2344)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     2939..2967
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(3081..3319)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(3113..3134)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     enhancer        3624..4003
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4004..4207
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4204..4228
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5392..6387
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(6433..6466)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6937..6959)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7045..7185
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7100..7119
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7200..7217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7371..7959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7451..7470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8130..8921,8922..8990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8753..8772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8991..9095)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9163..9181
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
       61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
      121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca
      181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
      241 aacgcatgcc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attaacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagatgcatc
      781 ctcactgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
      841 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
      901 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac
      961 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg
     1021 tctgtccgat tgtctagtgt ctatgactga ttttatgcgc ctgcgtcggt actagttagc
     1081 taactagctc tgtatctggc ggacccgtgg tggaactgac gagttcggaa cacccggccg
     1141 caaccctggg agacgtccca gggacttcgg gggccgtttt tgtggcccga cctgagtcca
     1201 aaaatcccga tcgttttgga ctctttggtg cacccccctt agaggaggga tatgtggttc
     1261 tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
     1321 gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc
     1381 tgactgtgtt tctgtatttg tctgaaaata tgggcccggg ccagactgtt accactccct
     1441 taagtttgac cttaggtcac tggaaagatg tcgagcagat cgctcacaac cagtcggtag
     1501 atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg
     1561 gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct
     1621 tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct
     1681 tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc
     1741 ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct
     1801 ccctttatcc agccctcact ccttctctag gcgcccccat atggccatat gagatcttat
     1861 atggggcacc cccgcccctt gtaaacttcc ctgaccctga catgacaaga gttactaaca
     1921 gcccctctct ccaagctcac ttacaggctc tctacttagt ccagcacgaa gtctggagac
     1981 ctctggcggc agcctaccaa gaacaactgg accgaccggt ggtacctcac ccttaccgag
     2041 tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag
     2101 gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt
     2161 ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagagaac
     2221 cccttcatcg agataacttc gtataaagtc tcctatacga agttatatcg atggccctca
     2281 ttatttgtag agctcatcca tgccatgtgt aatcccagca gcagttacaa actcaagaag
     2341 gaccatgtgg tcacgctttt cgttgggatc tttcgaaagg gcagattgtg tcgacaggta
     2401 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
     2461 tgctagttga acggatccat cttcaatgtt gtgacgaatt ttgaagttag ctttgattcc
     2521 attcttttgt ttgtctgccg tgatgtatac attgtgtgag ttatagttgt actcgagttt
     2581 gtgtccgaga atgtttccat cttctttaaa atcaatacct tttaactcga tacgattaac
     2641 aagggtatca ccttcaaact tgacttcagc acgcgtcttg tagttcccgt catctttgaa
     2701 agatatagtg cgttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgccg
     2761 tttcatatga tccggataac gggaaaagca ttgaacacca taagagaaag tagtgacaag
     2821 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagctttcc
     2881 gtatgtagca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
     2941 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tgctagccat
     3001 tttttctacc ggtaccaagc ttaagtttaa acgctagctg tgtgtgagtt cttctttctc
     3061 ggtaacttgt tgaaagtatt cagagttctc ttcttgtatt caataattac ttcttggcag
     3121 atttcagtag ttgcagttga tttacttggt tgctggttac ttttaattga ttcactttaa
     3181 cttgcacttt actgcagatt gtttagcttg ttcagctgcg cttgtttatt tgcttagctt
     3241 tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc
     3301 gcctctattt atactccggc ggtcgagggt actcccgggt actgagcttt cagcaagaga
     3361 acaatgcact tgtccatcga gctttcagca agagaacaat gcacttgtcc atcgagcttt
     3421 cagcaagaga acaatgcact tgtccatcga gctaacgaga acaatgcact tagcggtatc
     3481 gagaacaatg cacttaatat gcggccgaga tcgttggggt tcgaatgcag atcccgtcga
     3541 ggagcttggc ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
     3601 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat
     3661 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     3721 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3781 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3841 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     3901 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     3961 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     4021 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4081 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     4141 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     4201 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     4261 ccatagaaga caccgggacc gatccagcct ccccggctag ccgccgccac catgaaaaag
     4321 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     4381 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     4441 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     4501 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     4561 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     4621 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     4681 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     4741 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     4801 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     4861 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     4921 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     4981 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     5041 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     5101 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     5161 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     5221 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     5281 gtcgcgtctg cgttcgacca ggctgcgcgt tctcgcggcc atagcaaccg acgtacggcg
     5341 ttgcgccctc gccggcagca agaagccacg gaagtccgcc cggagcagaa aatgcccacg
     5401 ctactgcggg tttatataga cggtccccac gggatgggga aaaccaccac cacgcaactg
     5461 ctggtggccc tgggttcgcg cgacgatatc gtctacgtac ccgagccgat gacttactgg
     5521 cgggtgctgg gggcttccga gacaatcgcg aacatctaca ccacacaaca ccgcctcgac
     5581 cagggtgaga tatcggccgg ggacgcggcg gtggtaatga caagcgccca gataacaatg
     5641 ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc atatcggggg ggaggctggg
     5701 agctcacatg ccccgccccc ggccctcacc ctcatcttcg accgccatcc catcgccgcc
     5761 ctcctgtgct acccggccgc gcggtacctt atgggcagca tgacccccca ggccgtgctg
     5821 gcgttcgtgg ccctcatccc gccgaccttg cccggcacca acatcgtgct tggggccctt
     5881 ccggaggaca gacacatcga ccgcctggcc aaacgccagc gccccggcga gcggctggac
     5941 ctggctatgc tggctgcgat tcgccgcgtt tacgggctac ttgccaatac ggtgcggtat
     6001 ctgcagtgcg gcgggtcgtg gcgggaggac tggggacagc tttcggggac ggccgtgccg
     6061 ccccagggtg ccgagcccca gagcaacgcg ggcccacgac cccatatcgg ggacacgtta
     6121 tttaccctgt ttcgggcccc cgagttgctg gcccccaacg gcgacctgta taacgtgttt
     6181 gcctgggcct tggacgtctt ggccaaacgc ctccgttcca tgcacgtctt tatcctggat
     6241 tacgaccaat cgcccgccgg ctgccgggac gccctgctgc aacttacctc cgggatggtc
     6301 cagacccacg tcaccacccc cggctccata ccgacgatat gcgacctggc gcgcacgttt
     6361 gcccgggaga tgggggaggc taactgaagc ttcccggggg taccgaattc gtcgacagat
     6421 ccggccggcc ccataacttc gtatagcata cattatacga agttatcccc tgcagcggcc
     6481 gcgatccgag ttcttctgag cgggactctg gggttcgata aaataaaaga ttttatttag
     6541 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     6601 aacgccattt tgcaaggcat ggaaaaatac ataactgatc atccggatgc atacgtaggg
     6661 tcccggcgcg ccacgcgtcc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     6721 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     6781 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     6841 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     6901 tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6961 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7021 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     7081 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     7141 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     7201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7321 aaaggctagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7801 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     8461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     8761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9001 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9121 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     9181 ccctttcgtc ttcaa
//
