LOCUS       sequence-239474-        7323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""Activate neurons with 2p stimulation"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7323)
  TITLE     AAV plasmids expressing Red-shifted channelrhodopsins with soma 
            localization sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CamKIIa-C1V1::FusionRed::Kv2.1
FEATURES             Location/Qualifiers
     source          1..7323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   51..180
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        199..1487
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    3500..4088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3553..3573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3971..3982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4120..4596
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4319..4338)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4636..4776
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4636..4765
                     /label=AAV2 ITR
     rep_origin      4851..5306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4938..4957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5148..5169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5323..5342)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5442..5464
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5502..5520)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5588..5692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5693..5761,5762..6553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5911..5930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6724..7312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7213..7232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
       61 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      121 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      181 gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg ggttcttctt
      241 ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc tgcccagggt
      301 cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa attccatgcc
      361 ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag atgaccaatg
      421 agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca gccctgggtg
      481 agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt gacgggaagc
      541 acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc ctctgggagg
      601 atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca gtggtgacag
      661 ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt ttggtacttc
      721 ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg agagctggaa
      781 agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag gaagcaaaga
      841 aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt cccctgtaaa
      901 ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca gaggagatga
      961 agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg tggcacccac
     1021 tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt ctgttaaaag
     1081 ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg gactaagttt
     1141 gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag ggtccacttg
     1201 ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa agaggagcag
     1261 gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa cgggaacagg
     1321 gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc gaggctgtga
     1381 gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt tctccgtttg
     1441 cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct agagcggtac
     1501 cggatccccg ggtaccggtg ccaccatgag tcggcgaccc tggcttctcg cacttgcttt
     1561 ggctgtagct ttggcagctg gaagtgctgg ggctagtacc gggtctgacg ctactgtccc
     1621 tgtggccacc caggacggac cagattatgt cttccataga gcccacgaga gaatgctttt
     1681 ccagactagc tatacccttg aaaataacgg tagcgtaatt tgcattccca ataacggcca
     1741 gtgtttctgc ctggcttggt tgaagtctaa cggcacaaac gccgaaaagc tggctgcaaa
     1801 tatacttcaa tggattacct tcgcattgag tgcattgtgt ctcatgttct atggttacca
     1861 aacctggaag agcacttgcg ggtgggagac tatctacgtt gctacaatag aaatgataaa
     1921 gttcataatt gaatacttcc atgaatttga tgaacctgcc gtcatttaca gttcaaatgg
     1981 taataaaacc gtttggttga gatacgcaac atggctgctg acttgccccg tcttgcttat
     2041 tcatctgagt aatctgactg gtctgaagga cgactattct aaacgaacaa tggggctcct
     2101 cgtcagtgat gtgggctgta tcgtctgggg cgcaaccagc gctatgtgta ccgggtggac
     2161 aaaaatactg ttcttcctta ttagcctgtc ttatgggatg tacacctact tccacgctgc
     2221 taaagtctat atcgaagcct ttcacactgt ccccaagggc atatgtcggg aactggtaag
     2281 ggtgatggct tggaccttct ttgttgcttg gggcatgttc cctgttttgt ttctgttggg
     2341 caccgaggga tttggtcata tttctccata tggaagcgct atcggccatt caatccttga
     2401 tctcatagct aaaaacatgt ggggtgtgct ggggaattac ttgcgagtta aaattcatga
     2461 gcatattctt ctttatggcg acataagaaa aaaacaaaag atcactattg cagggcaaga
     2521 aatggaggtc gaaacccttg ttgcagagga ggaggacgcc gctccagtag tagctgtgag
     2581 cgaactcata aaggagaata tgcctatgaa actttacatg gaagggaccg taaacaatca
     2641 tcacttcaaa tgtacttccg aaggtgaggg taaaccttat gaaggcacac aaacaatgag
     2701 gattaaggtg gttgaaggtg gccccttgcc attcgctttt gacattttgg caacaagctt
     2761 catgtacggc tcacgaacat ttattaaaca cccccccggt attcctgact tctttaagca
     2821 aagcttccct gagggtttta catgggaacg ggtaaccacc tacgaggacg gcggcgttct
     2881 gacagcaaca caagacacaa gtctgcagga cgggtgtctc atctacaatg tgaaagtaag
     2941 aggtgtcaat ttccccgcta acggtcctgt aatgcaaaag aaaacactcg ggtgggaggc
     3001 ttccacagaa acaatgtatc cagccgacgg aggcttggag ggggcctgcg acatggcctt
     3061 gaagcttgta gggggagggc atctcatatg taacctcgag acaacttaca ggagcaagaa
     3121 acccgccact aatttgaaga tgcccggggt ctacaacgtt gatcaccggc ttgagcgaat
     3181 caaagaggct gacgacgaaa cctatgtaga acaacacgaa gttgccgtgg cccgatatag
     3241 caccgggggt gccggtgatg gtgggaagta caaacaaagc cagcccatcc ttaacactaa
     3301 agagatggct ccacaatcaa aaccacccga ggagcttgaa atgagctcca tgccttctcc
     3361 tgtagctccc ctccccgcaa gaacagaagg ggtgattgac atgcgctcta tgagcagtat
     3421 agacagcttc atctcatgtg ctaccgactt cccagaagca acaaggttta agtaagaatt
     3481 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3541 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3601 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3661 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3721 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccggaac
     3781 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3841 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3901 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
     3961 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4021 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4081 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     4141 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     4201 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     4261 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     4321 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     4381 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4441 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4501 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4561 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     4621 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4681 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4741 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4801 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4861 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4921 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4981 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5041 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     5101 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5161 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     5221 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5281 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     5341 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5401 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5461 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5521 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5581 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5641 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5701 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5761 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5821 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5881 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5941 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6001 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6061 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6121 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6181 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6241 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6301 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6361 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6421 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6481 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6541 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6601 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6661 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6721 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6781 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6841 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6901 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6961 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7021 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7081 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7141 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7201 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7261 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7321 caa
//
