LOCUS       sequence-239483-        4581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Klf4 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1395_pGL4-UBCmin-luc2/Klf4-B'
FEATURES             Location/Qualifiers
     source          1..4581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    12..60
                     /note="synthetic polyadenylation signal"
     misc_feature    74..165
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     114..133
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      347..356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             353..2005
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2049..2170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2060..2079)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2114..2133
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2669..2686)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2840..3428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2920..2939)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3628..4419,4420..4488))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
       61 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
      121 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctggcct aactggccgg
      181 tacctgagct cgctagcctc gaggatgggg cggtgaacgc cgatgattat ataaggacgc
      241 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gaattcctcg actctagagg
      301 atctggcctc ggcggccaag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga
      361 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg
      421 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac
      481 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct
      541 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga
      601 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc
      661 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc
      721 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct
      781 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag
      841 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc
      901 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg
      961 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg
     1021 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt
     1081 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt
     1141 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca
     1201 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa
     1261 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt
     1321 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac
     1381 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg
     1441 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg
     1501 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa
     1561 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat
     1621 cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa
     1681 atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa
     1741 catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc
     1801 agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc
     1861 cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc
     1921 taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa
     1981 gaagggcggc aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc
     2041 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2101 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2161 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2221 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgcg
     2281 cccacatttc ccaaaaatga ggaggttgcc aagttgaaat tgatgagtgt gtaattaaat
     2341 gtctacaaaa ggtaacatta tgtcaactgt ctttgcaact ggcctcccca gttagctata
     2401 tataactcat ttgatatgca aagtgggtga atttcaatgt gtgtgatagg atgtgggagt
     2461 ggggccttgg aaaataacag tgccctgggg cctccaaggt caaaaacaat cctagccctt
     2521 cccaccctgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2581 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2641 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2701 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2761 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2821 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2881 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2941 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3001 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3061 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3121 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3181 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3241 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3301 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3361 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3421 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3481 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3541 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     3601 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     3661 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3721 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3781 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3841 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     3901 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     3961 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     4021 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     4081 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     4141 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     4201 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     4261 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     4321 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     4381 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     4441 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4501 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     4561 tattaaggta cgggaggtat t
//
