LOCUS       sequence-239489-        4630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Klf4 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1396_pGL4-UBCmin-luc2/Klf4-E'
FEATURES             Location/Qualifiers
     source          1..4630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    53..101
                     /note="synthetic polyadenylation signal"
     misc_feature    115..206
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     155..174
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             394..2046
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2090..2211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2101..2120)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2155..2174
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2759..2776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2930..3518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3010..3029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3718..4509,4510..4578))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat
       61 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
      121 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
      181 aagtgcaggt gccagaacat ttctctggcc taactggccg gtacctgagc tcgctagcct
      241 cgaggatggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta
      301 gttccgtcgc agccgggatt tgaattcctc gactctagag gatctggcct cggcggccaa
      361 gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag
      421 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
      481 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
      541 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
      601 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
      661 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
      721 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
      781 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
      841 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
      901 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
      961 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     1021 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     1081 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     1141 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     1201 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     1261 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     1321 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     1381 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     1441 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     1501 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     1561 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     1621 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     1681 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     1741 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     1801 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     1861 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     1921 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     1981 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     2041 gtgtaataat tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt
     2101 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     2161 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     2221 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     2281 taaaacctct acaaatgtgg taaaatcgat aaggatctag tccaatgtca aattccggac
     2341 aaattcctag aagtggcctc aaaagctctc cccacgaatt aacgactctg atcttcatat
     2401 aaatggagaa acccccgcac tttgttggtc agctgctttc ccttataata atgtattcaa
     2461 ggttagcagg ttatagtcca tcctttgcca ggaaggttgc tcaggattga acaggcaagg
     2521 atgacctcca cacaacttca ttctttttaa aattctatta gggatccagg ccacattttc
     2581 ctgatccatt catcaccctg tagacacgtg cactacctgt cgaccgatgc ccttgagagc
     2641 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     2701 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     2761 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2821 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3361 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3661 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta
     3721 ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt
     3781 ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag
     3841 cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca
     3901 gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc
     3961 tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt
     4021 tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa
     4081 ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt
     4141 cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat
     4201 ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt
     4261 gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc
     4321 ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat
     4381 catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag
     4441 ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt
     4501 ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg
     4561 aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta
     4621 ctagtacgtc
//
