LOCUS       sequence-239494-        2311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli expression vector for CasX sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, 
            Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, 
            Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA
  TITLE     CasX enzymes comprise a distinct family of RNA-guided genome 
            editors.
  JOURNAL   Nature. 2019 Feb 4. pii: 10.1038/s41586-019-0908-x. doi: 
            10.1038/s41586-019-0908-x.
  PUBMED    30718774
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Feb 4. pii: 10.1038/s41586-019-0908-x. doi: 
            10.1038/s41586-019-0908-x."
COMMENT     Sequence Label: pBLO2 casX
FEATURES             Location/Qualifiers
     source          1..2311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..50
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(281..298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(532..551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1211..2002,2003..2071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1834..1853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2072..2176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2244..2262
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2266..2309)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattctaaa gatctttgac agctagctca gtcctaggta taatactagt acatctggcg
       61 cgtttattcc attactttgg agccagtccc agcgactatg tcgtatggac gaagcgctta
      121 tttatcggag agaaaccgat aagtaaaacg catcaaagct ggagttgtcc caattcttct
      181 agagtcacac tggctcacct tcgggtgggc cctagagtca cactggctca ccttcgggtg
      241 tttctgcggg atccttactc gagtctagac tgcaggcttc ctcgctcact gactcgctgc
      301 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      361 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
      481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2221 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     2281 aatccttagc tttcgctaag gatgatttct g
//
