LOCUS       sequence-239520-        4653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Klf4 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1401_pGL4-UBCmin-luc2/Klf4-F'
FEATURES             Location/Qualifiers
     source          1..4653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    55..103
                     /note="synthetic polyadenylation signal"
     misc_feature    117..208
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     157..176
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      390..399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             396..2048
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2092..2213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2103..2122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2157..2176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2784..2801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2955..3543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3035..3054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3743..4534,4535..4603))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa
       61 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
      121 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
      181 gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtacctga gctcgctagc
      241 ctcgaggatg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      301 tagttccgtc gcagccggga tttgaattcc tcgactctag aggatctggc ctcggcggcc
      361 aagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
      421 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
      481 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
      541 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
      601 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
      661 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
      721 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
      781 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
      841 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
      901 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
      961 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
     1021 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
     1081 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
     1141 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
     1201 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
     1261 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
     1321 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
     1381 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
     1441 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
     1501 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
     1561 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
     1621 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     1681 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
     1741 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
     1801 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
     1861 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
     1921 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
     1981 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
     2041 ccgtgtaata attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     2101 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     2161 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2221 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2281 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc aattgactat tctagggagc
     2341 gcgtaaaatg acaaaggact ttggactagt gaatgtgatt agtattcaaa agctgggagg
     2401 actttaaagg gtacggaaga atagggaaca agctgagaaa gtgaactaag aatcaagtgc
     2461 tcagtggggc ttatcttcat ttcccctctc cctcccaccg aggctgagat ccaccgggtg
     2521 gaccagaagc ttgcctgcag ggcctgggag agattgtaaa aagaattgcc ctgggggctg
     2581 aggggggggg acggaaaagg tctttagaag gaacaggcag gcggcggttg gtcaacaaca
     2641 atggtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     2701 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     2761 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2821 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3541 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3601 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3661 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagcggccgc
     3721 aaatgctaaa ccactgcagt ggttaccagt gcttgatcag tgaggcaccg atctcagcga
     3781 tctgcctatt tcgttcgtcc atagtggcct gactccccgt cgtgtagatc actacgattc
     3841 gtgagggctt accatcaggc cccagcgcag caatgatgcc gcgagagccg cgttcaccgg
     3901 cccccgattt gtcagcaatg aaccagccag cagggagggc cgagcgaaga agtggtcctg
     3961 ctactttgtc cgcctccatc cagtctatga gctgctgtcg tgatgctaga gtaagaagtt
     4021 cgccagtgag tagtttccga agagttgtgg ccattgctac tggcatcgtg gtatcacgct
     4081 cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg gtcaagccgg gtcacatgat
     4141 cacccatatt atgaagaaat gcagtcagct ccttagggcc tccgatcgtt gtcagaagta
     4201 agttggccgc ggtgttgtcg ctcatggtaa tggcagcact acacaattct cttaccgtca
     4261 tgccatccgt aagatgcttt tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt
     4321 agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat acgggacaac accgcgccac
     4381 atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa
     4441 ggatcttgcc gctattgaga tccagttcga tatagcccac tcttgcaccc agttgatctt
     4501 cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg
     4561 caaagaaggg aatgagtgcg acacgaaaat gttggatgct catactcgtc ctttttcaat
     4621 attattgaag catttatcag ggttactagt acg
//
