LOCUS       sequence-239524-        4526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Nanog enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1402_pGL4-UBCmin-luc2/Nanog-A'
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    20..68
                     /note="synthetic polyadenylation signal"
     misc_feature    82..173
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     122..141
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             361..2013
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2057..2178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2068..2087)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2122..2141
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2622..2639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2793..3381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2873..2892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3581..4372,4373..4441))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
       61 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
      121 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa
      181 ctggccggta cctgagctcg ctagcctcga ggatggggcg gtgaacgccg atgattatat
      241 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttga attcctcgac
      301 tctagaggat ctggcctcgg cggccaagct tggcaatccg gtactgttgg taaagccacc
      361 atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg
      421 accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc
      481 gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc
      541 gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg
      601 tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg
      661 gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc
      721 agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa
      781 aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc
      841 ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa cgagtacgac
      901 ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggc
      961 agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt
     1021 catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat cctcagcgtg
     1081 gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt
     1141 cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat
     1201 aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc
     1261 atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc
     1321 aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac
     1381 ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc
     1441 gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag
     1501 acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc
     1561 tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc
     1621 ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc
     1681 ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa
     1741 caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg
     1801 cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac
     1861 tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac
     1921 gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt
     1981 aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg cggccggccg
     2041 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2101 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2161 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2221 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     2281 gatccagaga tcgccagggt ctggaggtgc agccgtggtt aaaagatgaa taaagtgaaa
     2341 tgaggtaaag cctctttttg gggggggggg atgtctttag atcagaggat gccccctaag
     2401 ctttccctcc ctcccagtct gggtcacctt acagcttctt ttgcattaca atgtccatgg
     2461 tggaccctgc aggtgggatt agtcgaccga tgcccttgag agccttcaac ccagtcagct
     2521 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     2581 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
     2641 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2701 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2761 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2821 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2881 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2941 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3001 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3061 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3421 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3481 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3541 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
     3601 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
     3661 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
     3721 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
     3781 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
     3841 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
     3901 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
     3961 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
     4021 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
     4081 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
     4141 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
     4201 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
     4261 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
     4321 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
     4381 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
     4441 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
     4501 gataagtaag taatattaag gtacgg
//
