LOCUS       sequence-239526-        4769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Pou5f1 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4
FEATURES             Location/Qualifiers
     source          1..4769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    25..73
                     /note="synthetic polyadenylation signal"
     misc_feature    87..178
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     127..146
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      360..369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             366..2018
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2062..2183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2073..2092)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2127..2146
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2870..2887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3041..3629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3121..3140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3829..4620,4621..4689))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tacgggaggt attggacagg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
       61 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
      121 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctgg
      181 cctaactggc cggtacctga gctcgctagc ctcgaggatg gggcggtgaa cgccgatgat
      241 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgaattcc
      301 tcgactctag aggatctggc ctcggcggcc aagcttggca atccggtact gttggtaaag
      361 ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag
      421 acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca
      481 ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga
      541 tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg
      601 tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg
      661 gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg
      721 gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg
      781 tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc
      841 agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt
      901 acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta
      961 gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat
     1021 tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca
     1081 gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg
     1141 gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag
     1201 actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca
     1261 ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc
     1321 tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg
     1381 gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc
     1441 ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg
     1501 gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga
     1561 gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc
     1621 acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga
     1681 agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc
     1741 tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg
     1801 agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg
     1861 tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg
     1921 tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc
     1981 tcattaaggc caagaagggc ggcaagatcg ccgtgtaata attctagagt cggggcggcc
     2041 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2101 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2161 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2221 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     2281 ataaggatcc ttgtcctggc cagtgagtca ccaaaagaga aatcacaatc cataagacaa
     2341 ggttggtatt gaatacagac aggactgctg ggctgcaggc atacttgaac tgtggtggag
     2401 agtgctgtct aggccttaga ggctggccct gggaggaact gggtgtgggg aggttgtagc
     2461 ccgaccctgc ccctcccccc agggaggttg agagttctgg gcagacggca gatgcataac
     2521 aaaggtgcat gatagctctg ccctgggggc agagaagatg gttggggagg ggtccctctc
     2581 gtcctagccc ttccttaatc tgctattgag gaagctttgt gaacttggcg gcttccaagt
     2641 cgctgccttt atttaggtct tccaactaac ctatggcact gttccacaat gaatgtatag
     2701 aaattgggag gtgagcatga cagagtggag tcgaccgatg cccttgagag ccttcaaccc
     2761 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     2821 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     2881 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2941 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3001 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3061 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3121 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3181 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3241 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3301 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3361 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3421 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3481 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3541 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3601 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3661 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3721 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3781 agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt accagtgctt
     3841 gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag tggcctgact
     3901 ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca gcgcagcaat
     3961 gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc agccagcagg
     4021 gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt ctatgagctg
     4081 ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag ttgtggccat
     4141 tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca actctggttc
     4201 ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag tcagctcctt
     4261 agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc
     4321 agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga
     4381 gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct cttgcccggc
     4441 gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca tcatcgggaa
     4501 tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca gttcgatata
     4561 gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg tttcggggtg
     4621 tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg
     4681 gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt actagtacgt
     4741 ctctcaagga taagtaagta atattaagg
//
