LOCUS       sequence-239527-        4690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Pou5f1 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3
FEATURES             Location/Qualifiers
     source          1..4690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    29..77
                     /note="synthetic polyadenylation signal"
     misc_feature    91..182
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     131..150
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      364..373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             370..2022
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2066..2187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2077..2096)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2131..2150
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2795..2812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2966..3554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3046..3065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3754..4545,4546..4614))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg
       61 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
      121 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
      181 ctggcctaac tggccggtac ctgagctcgc tagcctcgag gatggggcgg tgaacgccga
      241 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttgaa
      301 ttcctcgact ctagaggatc tggcctcggc ggccaagctt ggcaatccgg tactgttggt
      361 aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc
      421 gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc
      481 ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc
      541 gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg
      601 atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc
      661 atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc
      721 atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc
      781 aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac
      841 taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac
      901 gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac
      961 agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc
     1021 cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc
     1081 ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc
     1141 tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg
     1201 caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag
     1261 agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg
     1321 ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc
     1381 cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac
     1441 aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac
     1501 accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc
     1561 atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg
     1621 ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg
     1681 ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc
     1741 ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc
     1801 ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag
     1861 atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg
     1921 ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag
     1981 attctcatta aggccaagaa gggcggcaag atcgccgtgt aataattcta gagtcggggc
     2041 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     2101 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2161 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2221 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     2281 atcgataagg atctgaccct agccaacagc tcaggcgggc tgggcccagg ctcagaactc
     2341 tgtcctggct atgtacactg tggggtgctc tgggcttttt gaggctgtgt gattcaccct
     2401 ggggccttcg ttcagagcat ggtgtaggag cagacagaca aacaccatcc cttgcagaca
     2461 ggcactctga gggctattct cttgcaaaga taactaagca ccaggccagt aatgggatcc
     2521 tcagactggg cccagaaaac cactctaggg aagttcaggg taggctctct gcaccccctc
     2581 ctcctaatcc cgtctcctta gtgtctttcc gccagcacag gaatggggga ggggtgggtg
     2641 acgaggatga acacgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     2701 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     2761 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3721 acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc
     3781 gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat
     3841 cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc
     3901 gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag
     3961 aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag
     4021 agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt
     4081 ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg
     4141 ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt
     4201 tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc
     4261 tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc
     4321 gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa
     4381 caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg
     4441 gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc
     4501 cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa
     4561 gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt
     4621 cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt
     4681 aagtaatatt
//
