LOCUS       sequence-239528-        4765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Pou5f1 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2
FEATURES             Location/Qualifiers
     source          1..4765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    49..97
                     /note="synthetic polyadenylation signal"
     misc_feature    111..202
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     151..170
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             390..2042
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2086..2207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2097..2116)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2151..2170
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2890..2907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3061..3649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3141..3160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3849..4640,4641..4709))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct
       61 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
      121 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
      181 gcaggtgcca gaacatttct ctggcctaac tggccggtac ctgagctcgc tagcctcgag
      241 gatggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
      301 cgtcgcagcc gggatttgaa ttcctcgact ctagaggatc tggcctcggc ggccaagctt
      361 ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc
      421 cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga
      481 agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca
      541 ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg
      601 ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc
      661 ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg
      721 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
      781 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
      841 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
      901 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
      961 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
     1021 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
     1081 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
     1141 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
     1201 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
     1261 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
     1321 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
     1381 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
     1441 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
     1501 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
     1561 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
     1621 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
     1681 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1741 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1801 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     1861 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     1921 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     1981 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt
     2041 aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg
     2101 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     2161 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2221 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     2281 acctctacaa atgtggtaaa atcgataagg atccggggtg ggtgacgagg atgaacaccg
     2341 gagtccctgg aggaagggaa gcagggtatc tccatctgag gctctgtctt tgaggagagg
     2401 tggagagctg gggaagtctt gtgtgagggg attggggctc aggagggggt tggggagcag
     2461 gaagttgtcc ccaggggagc catcctggcc cattcaaggg ttgagtactt gtttagggtt
     2521 agagctgccc cctctgggga ccaggattgt ccagccaagg ccattgtcct gcccccttcc
     2581 cccagtccct cccaggcccc tttgaacctg aagtcagata tttcttctct ctacccacct
     2641 cccacccgtt gggtttctcc acccaggaac taggctggaa gcctgggatg aggaggtggg
     2701 gggagggaga actgagaatc ttgaggaaag aggccccggc cttaactgtg tcgaccgatg
     2761 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     2821 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     2881 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2941 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3001 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3061 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3541 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3781 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     3841 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     3901 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     3961 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     4021 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     4081 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     4141 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     4201 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     4261 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     4321 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     4381 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     4441 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     4501 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     4561 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     4621 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     4681 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     4741 tatcagggtt actagtacgt ctctc
//
