LOCUS       sequence-239530-        4665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Fgf4 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1869_pGL4-UBCmin-luc2/Fgf4-A'
FEATURES             Location/Qualifiers
     source          1..4665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    36..84
                     /note="synthetic polyadenylation signal"
     misc_feature    98..189
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     138..157
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             377..2029
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2073..2194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2084..2103)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2138..2157
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2777..2794)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2948..3536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3028..3047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3736..4527,4528..4596))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
       61 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
      121 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
      181 catttctctg gcctaactgg ccggtacctg agctcgctag cctcgaggat ggggcggtga
      241 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      301 atttgaattc ctcgactcta gaggatctgg cctcggcggc caagcttggc aatccggtac
      361 tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
      421 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
      481 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
      541 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
      601 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
      661 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
      721 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
      781 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
      841 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
      901 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
      961 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     1021 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     1081 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     1141 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     1201 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     1261 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     1321 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     1381 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
     1441 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
     1501 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
     1561 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
     1621 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
     1681 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
     1741 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
     1801 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
     1861 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
     1921 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
     1981 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag
     2041 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     2101 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2161 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2221 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2281 tggtaaaatc gataaggatc cgcttcaggg aatgcttctc cccaagatgt taagactctg
     2341 ctgggagact tctgagccac ctcccgaatt aactttatgg gaggctacag acagcaagac
     2401 tggaaaatct gattggcatt tttttttttt tttgtctttc acattccttt agaaaactct
     2461 ttgtttggat gctaatggat acttaaaata ctattctgta ccacagccca agatggaaga
     2521 agccacgccc caaagctgag gtgggagctc ctcccaaact tcctttctgt ctggtggctc
     2581 acaggacaat aagattttgt gttttttaaa tccaggccct aggctccaag agtgttgtcg
     2641 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     2701 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     2761 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3361 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct
     3721 aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct
     3781 atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg
     3841 cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga
     3901 tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt
     3961 gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt
     4021 gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt
     4081 cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat
     4141 attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc
     4201 cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc
     4261 cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat
     4321 acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag
     4381 tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt
     4441 gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc
     4501 ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa
     4561 gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg
     4621 aagcatttat cagggttact agtacgtctc tcaaggataa gtaag
//
