LOCUS       sequence-239531-        4808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Esrrb enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1870_pGL4-UBCmin-luc2/Esrrb-B'
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    40..88
                     /note="synthetic polyadenylation signal"
     misc_feature    102..193
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     142..161
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      375..384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             381..2033
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2077..2198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2088..2107)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2142..2161
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2924..2941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3095..3683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3175..3194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3883..4674,4675..4743))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca
       61 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
      121 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
      181 agaacatttc tctggcctaa ctggccggta cctgagctcg ctagcctcga ggatggggcg
      241 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
      301 cgggatttga attcctcgac tctagaggat ctggcctcgg cggccaagct tggcaatccg
      361 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      421 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      481 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
      541 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
      601 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
      661 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
      721 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
      781 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
      841 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
      901 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
      961 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
     1021 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
     1081 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
     1141 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
     1201 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1261 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     1321 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     1381 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     1441 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1501 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     1561 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     1621 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     1681 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     1741 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     1801 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     1861 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     1921 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     1981 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct
     2041 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
     2101 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     2161 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     2221 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     2281 aatgtggtaa aatcgataag gatccaggga taactcttta ctgccacacc ctttgaaaat
     2341 ggagatgttt accccctctg tccctagtag cccagacgag cctcaaactt ggctgtatag
     2401 ctgagcatga ccttgaactg ttgctccttc tgtcccggat tcccaagggc tggcattacc
     2461 ggctggtatc acctgattta cgaggttgct ttcttttgct ggtggtattc aactgcaaag
     2521 ttgagctatc aagtcattgg caaagaggac aaagcctttg gatttggagg tggcccacga
     2581 attccacaga tccgggaagc acaggcccac gtcctccact ccaaaggatc attaacagat
     2641 cctgactgta gtggtgctga cggcagggag tatagaaccc acaacgtgac cggaacaagc
     2701 ctgtggtgat tgcccggaaa gcagccaggc cacctgcagc atccctgctt caaggtcaac
     2761 tgaaaagcct gggctccagc agagtcgacc gatgcccttg agagccttca acccagtcag
     2821 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     2881 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     2941 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3001 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3061 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3841 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     3901 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     3961 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     4021 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     4081 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     4141 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     4201 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     4261 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     4321 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     4381 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     4441 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     4501 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     4561 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     4621 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     4681 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     4741 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     4801 aggataag
//
