LOCUS       sequence-239532-       11791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; 
            C-terminal truncation; QES mutant for enhanced presentation of 
            quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH115gp160-QES.c14-754*
FEATURES             Location/Qualifiers
     source          1..11791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1312..3101
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3661..3683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3826..3960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3863..3882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3917..3936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6284..6308)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6305..6508)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6334..6354)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6509..6888
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7542..7590
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7615..7634
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7632..8669)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8712..8857)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(9288..9876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9368..9387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10047..10838,10839..10907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10670..10689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10908..11012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11080..11098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtggaacagg agcaggagca ggagcgggag gggcaggagc aggaggggca ggagcaggag
       61 gwggrggccg gggtcgagga ggcagtggag gccggggtcg aggaggtagt ggaggccggg
      121 gtcgaggagg tagtggaggc cgccggggta gaggacgtga aagagccagg gggggaagtc
      181 gtgaaagagc cagggggaga ggtcgtggac gtggagaaaa gaggcccagg agtcccagta
      241 gtcagtcatc atcatccggg tctccaccgc gcaggccccc tccaggtaga aggccatttt
      301 tccaccctgt aggggaagcc gattattttg aataccacca agaaggtggc ccagatggtg
      361 agcctgacgt gcccccggga gcgatagagc agggccccgc agatgaccca ggagaaggcc
      421 caagcactgg accccggggt cagggtgatg gaggcaggcg caaaaaagga gggtggtttg
      481 gaaagcatcg tggtcaagga ggttccaacc cgaaatttga gaacattgca gaaggtttaa
      541 gagctctcct ggctaggagt cacgtagaaa ggactaccga cgaaggaact tgggtcgccg
      601 gtgtgttcgt atatggaggt agtaagacct ccctttacaa cctaaggcga ggaactgccc
      661 ttgctattcc acaatgtcgt cttacaccat tgagtcgtct cccctttgga atggcccctg
      721 gacccggccc acaacctggc ccgctaaggg agtccattgt ctgttatttc atggtctttt
      781 tacaaactca tatatttgct gaggttttga aggatgcgat taaggacctt gttatgacaa
      841 agcccgctcc tacctgcaat atcagggtga ctgtgtgcag ctttgacgat ggagtagatt
      901 tgcctccctg gtttccacct atggtggaag gggctgccgc ggagggtgat gacggagatg
      961 acggagatga aggaggtgat ggagatgagg gtgaggaagg gcaggagtga tgtaacttgt
     1021 taggagacgc cctcaatcgt attaaaagcc gtgtattccc ccgcactaaa gaataaatcc
     1081 ccagtagaca tcatgcgtgc tgttggtgta tttctggcca tctgtcttgt caccattttc
     1141 gtcctcccaa catggggcaa ttgggcatac ccatgttgtc acgtcactca gctccgcgct
     1201 caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa tcagacatgc
     1261 gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca gcaggaaaag
     1321 gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa ggatagcact
     1381 cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag catatgctac
     1441 ccggatacag attaggatag catatactac ccagatatag attaggatag catatgctac
     1501 ccagatatag attaggatag cctatgctac ccagatataa attaggatag catatactac
     1561 ccagatatag attaggatag catatgctac ccagatatag attaggatag cctatgctac
     1621 ccagatatag attaggatag catatgctac ccagatatag attaggatag catatgctat
     1681 ccagatattt gggtagtata tgctacccag atataaatta ggatagcata tactacccta
     1741 atctctatta ggatagcata tgctacccgg atacagatta ggatagcata tactacccag
     1801 atatagatta ggatagcata tgctacccag atatagatta ggatagccta tgctacccag
     1861 atataaatta ggatagcata tactacccag atatagatta ggatagcata tgctacccag
     1921 atatagatta ggatagccta tgctacccag atatagatta ggatagcata tgctatccag
     1981 atatttgggt agtatatgct acccatggca acattagccc accgtgctct cagcgacctc
     2041 gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg tgtaatttgt
     2101 cctccagatc gcagcaatcg cgcccctatc ttggcccgcc cacctactta tgcaggtatt
     2161 ccccggggtg ccattagtgg ttttgtgggc aagtggtttg accgcagtgg ttagcggggt
     2221 tacaatcagc caagttatta cacccttatt ttacagtcca aaaccgcagg gcggcgtgtg
     2281 ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac ttgtctttgt
     2341 ttatgggccc cattggcgtg gagccccgtt taattttcgg gggtgttaga gacaaccagt
     2401 ggagtccgct gctgtcggcg tccactctct ttccccttgt tacaaataga gtgtaacaac
     2461 atggttcacc tgtcttggtc cctgcctggg acacatctta ataaccccag tatcatattg
     2521 cactaggatt atgtgttgcc catagccata aattcgtgtg agatggacat ccagtcttta
     2581 cggcttgtcc ccaccccatg gatttctatt gttaaagata ttcagaatgt ttcattccta
     2641 cactagtatt tattgcccaa ggggtttgtg agggttatat tggtgtcata gcacaatgcc
     2701 accactgaac cccccgtcca aattttattc tgggggcgtc acctgaaacc ttgttttcga
     2761 gcacctcaca tacaccttac tgttcacaac tcagcagtta ttctattagc taaacgaagg
     2821 agaatgaaga agcaggcgaa gattcaggag agttcactgc ccgctccttg atcttcagcc
     2881 actgcccttg tgactaaaat ggttcactac cctcgtggaa tcctgacccc atgtaaataa
     2941 aaccgtgaca gctcatgggg tgggagatat cgctgttcct taggaccctt ttactaaccc
     3001 taattcgata gcatatgctt cccgttgggt aacatatgct attgaattag ggttagtctg
     3061 gatagtatat actactaccc gggaagcata tgctacccgt ttagggttaa caagggggcc
     3121 ttataaacac tattgctaat gccctcttga gggtccgctt atcggtagct acacaggccc
     3181 ctctgattga cgttggtgta gcctcccgta gtcttcctgg gcccctggga ggtacatgtc
     3241 ccccagcatt ggtgtaagag cttcagccaa gagttacaca taaaggcaat gttgtgttgc
     3301 agtccacaga ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg caaatgtgca
     3361 catccattta taaggatgtc aactacagtc agagaacccc tttgtgtttg gtcccccccc
     3421 gtgtcacatg tggaacaggg cccagttggc aagttgtacc aaccaactga agggattaca
     3481 tgcactgccc cgcgaagaag gggcagagat gtcgtagtca ggtttagttc gtccggggcg
     3541 gggatcgatc ctctagagtc gacctcatgg ctgcgccccg acacccgcca acacccgctg
     3601 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3661 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagccgg
     3721 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3781 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     3961 cggccttgcc ggcctcgagt cattagagga atccagatac gaggcggatg gatctgtctt
     4021 tgtcctgttc gccgccttct tcttcaatgc gacccaaccg gtcaggaccg ccgctattag
     4081 gtataagtgt ctggaaggag agcggggagt atccttgcct tacccgattc actatggaga
     4141 gcacagcaaa aataatgcgc aagccgataa gcccccctac aatcattatg aatatcttaa
     4201 tataccacag ccagtttgtg atggagaacc aattccacaa atttttccat gagtccagag
     4261 ccaacaggtc cttctcattc ttttcctgtt gaatctgtga gtcttcaagc agggaatata
     4321 tgatgtcggt gtatgtgcta atctccttat cccattgcat ccatgtcgtg ccgttccaga
     4381 tgtctgtcaa ggacttgttg ctccatgaat cgttccacgg tacggcggtc gtgcaaataa
     4441 gttttccgct acaaccccag atccccagca attgctggtc ttgcaagtac cgctcaatgg
     4501 caagcaccct ggcctgcagc tgcttgatcc cccatacagt caattgaagc aaatgctgct
     4561 gagcttcgat cgctctaagc aggttgttct gttgctgtac gattccactc agaagttgcc
     4621 tcgcttgaac tgtgagcgtg agagacgccg cccccattgt ggatccagcg gctccaagga
     4681 atcccagaaa tacggcaccc aaacccaccg cgcgcttctc tcgttctacg accctccgct
     4741 tcgcgtcggt gggagctatg cccaagggct ttatttccac taccttatat ttataaagtt
     4801 cacttctcca attatctttc atgttacccc caagcggcct gaacgtctca gtattatttt
     4861 gcccaatgat attacccccg tccctgacga ggagcaggcc agtgatatta ctattacagg
     4921 ttatattgcc agcgattgga ggggcgtaca tggctctgcc tacttcttgc cacatattaa
     4981 tgatttgctt tattcggcaa ggaattgtta tagttgaatt gccttcggcc ccgataaaag
     5041 tgccgttggg catgtatgtg ccgttgaaga gacctgaagt gttgcaatag aaaaactcgc
     5101 ctctgcaatt gaaactgtga gttgtaattt cgagatctcc tcctgaagat gagttaaact
     5161 tgattgtctt attctgaaag tgctcagcga gttttttacc caccttttga agggtttcat
     5221 tccacctcgc cctgctgatg ttacagtgcg cctgtctaat gtcgccgatg atctctccag
     5281 tggcgtaaaa ggtttgcccc ggcccgattc tgatactctg tctagtgtta ttggagggcc
     5341 gtgtgcagtt gatttctaca gattgattca gatgtactat gattggcttt gcattgtctg
     5401 tcaagttctc tgatcgaatt attatctccc cctcggcgag gctaccgttc aagagaagct
     5461 gcgtagacat ggttggctta attccgtgcg tacactggac tgttgacaca ttatggcaag
     5521 gtccggttcc attgaacatc ttgtccttac acttgagtat cgcatatcct gctggagtgc
     5581 agtagtggat tgggatcgga tcgaaagtca tctttggaca cgcctgtgtt attgcactgg
     5641 tattacaatt gatgagcctg taactttcgg aagaattttc gctgctattt ttcttctcgt
     5701 ccaacggtac gatgtccaag cgataaaaga gtgcgtgcac cttttgcttt ttatctcgga
     5761 gctcggtggt agcgttgaag ctgcaatttt tcatctcttt gatggactca tggtgcgttc
     5821 tattctgggt gtcattacta cttgaaacat ttgaccgttc caaggttaca ttagagcatt
     5881 ccaatgtcac acacaagggc gtcagtttca cgcatggctt caagctctgg tcccaaagac
     5941 tgattacatc ttcatgcatt tgatccacca tcttattttc ccacatatta aagttctcag
     6001 taacattttt gagatcaatt tcgagggggt tggggtctgt aggtacgcag gcatgcgttg
     6061 cccaaacatt gtgaacttcc gcgtcataag ctttggcgtc ggaagcacag aaaagagtag
     6121 tggttgcctc tctccacact ggtacaccat agtacactgt aacccaaaga ttaccgacag
     6181 ccagcacact agcgaccaac atgccgagaa gatacagcgt agcgagcggt tgaagggagc
     6241 ccatcggcat ggtggcgcta gcagctggta cccagcttct agagatctga cggttcacta
     6301 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaacg
     6361 gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     6421 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     6481 cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     6541 actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag gcgggccatt
     6601 taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga tgtactgcca
     6661 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     6721 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     6781 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     6841 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa catggcggtc
     6901 atattggaca tgagccaata taaatgtaca tattatgata tagatacaac gtatgcaatg
     6961 gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct aattgccaat
     7021 attgattcaa tgtatagatc ttccatacct accagttctg cgcctgcagc aatggcaaca
     7081 acgttgcccg gatctgcgat gataagctgt caaacatgag aattggtcga ctagcttggc
     7141 acgccagaaa tccgcgcggt ggtttttggg ggtcgggggt gtttggcagc cacagacgcc
     7201 cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag gccatccaaa aaccatgggt
     7261 ctgtctgctc agtccagtcg tggacctgac cccacgcaac gcccaaaata ataaccccca
     7321 cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc cagtggctat
     7381 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     7441 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     7501 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     7561 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     7621 ccttccgtgt ttcagttagc ctcccccatc tcccgatccg gacgagtgct ggggcgtcgg
     7681 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     7741 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     7801 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     7861 ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
     7921 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     7981 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     8041 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     8101 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     8161 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     8221 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     8281 gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
     8341 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
     8401 caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg
     8461 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     8521 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     8581 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     8641 gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc tgcggcacgc
     8701 tgttgacgct gttaagcggg tcgctgcagg gtcgctcggt gttcgaggcc acacgcgtca
     8761 ccttaatatg cgaagtggac ctcggaccgc gccgccccga ctgcatctgc gtgttcgaat
     8821 tcgccaatga caagacgctg ggcggggttt gtgtcatcat agaactaaag acatgcaaat
     8881 atatttcttc cggggacacc gccagcaaac gcgagcaacg ggccacgggg atgaagcagg
     8941 gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     9001 aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
     9061 cagccgcacg cggcgcatct cggggccgac gcgctgggct acgtcttgct ggcgttcgcg
     9121 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     9181 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     9241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9721 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10021 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10081 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10141 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10201 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10261 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10321 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
    10381 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10441 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10501 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10561 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10621 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
    10681 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10741 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10801 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10861 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10921 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10981 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    11041 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    11101 tttcgtcttc aagaattctc atgtttgaca gcttatcatc gataagctga tcctcacagg
    11161 ccgcacccag cttttcttcc gttgccccag tagcatctct gtctggtgac cttgaagagg
    11221 aagaggaggg gtcccgagaa tccccatccc taccgtccag caaaaagggg gacgaggaat
    11281 ttgaggcctg gcttgaggct caggacgcaa atcttgagga tgttcagcgg gagttttccg
    11341 ggctgcgagt aattggtgat gaggacgagg atggttcgga ggatggggaa ttttcagacc
    11401 tggatctgtc tgacagcgac catgaagggg atgagggtgg gggggctgtt ggagggggca
    11461 ggagtctgca ctccctgtat tcactgagcg tcgtctaata aagatgtcta ttgatctctt
    11521 ttagtgtgaa tcatgtctga cgaggggcca ggtacaggac ctggaaatgg cctaggagag
    11581 aagggagaca catctggacc agaaggctcc ggcggcagtg gacctcaaag aagagggggt
    11641 gataaccatg gacgaggacg gggaagagga cgaggacgag gaggcggaag accaggagcc
    11701 ccgggcggct caggatcagg gccaagacat agagatggtg tccggagacc ccaaaaacgt
    11761 ccaagttgca ttggctgcaa agggacccac g
//
