LOCUS       sequence-239533-       11812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11812)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160-754*
FEATURES             Location/Qualifiers
     source          1..11812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(791..809)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        877..981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(982..1050,1051..1842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1200..1219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2013..2601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2502..2521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3032..3177
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3220..4257
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4255..4274)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4299..4347
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5001..5380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5381..5584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5535..5555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5581..5605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7592..7636
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    7914..8048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7938..7957)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7992..8011
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8191..8213)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8773..10562
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctcctgctc ctgccnnncc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
       61 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
      121 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
      181 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
      241 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
      301 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
      361 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
      421 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
      481 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
      541 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
      601 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
      661 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
      721 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
      781 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      841 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      961 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1021 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1081 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1141 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1201 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     1261 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1321 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1381 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1441 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1501 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     1561 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1621 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1681 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1741 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1801 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2641 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     2701 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     2761 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg
     2821 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     2881 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     2941 ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
     3001 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
     3061 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
     3121 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
     3181 cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     3241 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     3301 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     3361 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     3421 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     3481 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     3541 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     3601 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     3661 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     3721 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     3781 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     3841 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     3901 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     3961 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     4021 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     4081 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     4141 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     4201 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac
     4261 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4321 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4381 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     4441 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     4501 gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt
     4561 atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga
     4621 gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac
     4681 gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt
     4741 tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg
     4801 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
     4861 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     4921 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     4981 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     5041 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5101 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5161 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5221 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     5281 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     5341 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     5401 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     5461 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     5521 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     5581 agctcgttta gtgaaccgtc agatctctag aagctgggta ccagctgcta gcaagcttgc
     5641 tagcgccacc atgcccatgg ggagcttgca accgctcgcc actctctatc tcctcggcat
     5701 gttggtagca tctgtgctgg ctgaggaaaa gctctgggtg actgtgtatt acggggtccc
     5761 agtttggaag gaggctacta ccacgctctt ctgcgcctcc gaccggaagg catacgacac
     5821 cgaggtgcat aatgtgtggg ccacacacgc ctgtgtgcct accgacccca acccacagga
     5881 agtggaactg aagaacgtga ctgagaattt caatatgtgg aaaaataata tggtggagca
     5941 gatgcacgag gacattatta gcctctggga tcagtccctt aaaccctgcg tgaaactgac
     6001 accgctgtgc gtaacgctga attgtactga cctccggaat gccacgaacg gaaacgacac
     6061 aaataccacg tcaagctccc gagggatggt gggaggggga gaaatgaaga actgctcatt
     6121 caatataacg acgaatatcc gagggaaggt tcagaaagag tatgccctgt tttacaaact
     6181 ggacatcgct cctatcgaca acaacagcaa taacaggtac cgcctgatta gctgcaacac
     6241 ttctgtgatt acccaggcct gccccaaggt gagcttcgag cctatcccaa tccactactg
     6301 cgccccagca ggctttgcca tcctgaaatg caaggacaag aagttcaacg gcaaaggccc
     6361 atgtacaaat gtcagtacag tacagtgcac acacggtatc cgcccagttg tctctacaca
     6421 actgctgctg aatggatcct tggcagagga agaggtggtg atcagatctg ctaacttcgc
     6481 cgataatgct aaagtcatca ttgttcagct gaacgagtct gtcgaaatca attgtactcg
     6541 cccaaacaac aacacgcgca agagcattca tatcggaccg ggcagggcct tttacacaac
     6601 aggggagatt attggtgata ttagacaagc ccactgcaac ctcagtcgag caaagtggaa
     6661 tgatactctc aacaaaattg ttatcaaatt gcgggaacag tttgggaaca agacaatcgt
     6721 atttaagcac tcatccggag gcgatccaga aatcgtgaca cactctttta actgcggtgg
     6781 agaatttttc tactgcaact ccacccagct gttcaacagt acgtggaacg tgacagagga
     6841 gtctaacaac accgtggaga ataatacgat cacccttcca tgtcggatta agcagatcat
     6901 caatatgtgg caggaggtgg gaagagcaat gtacgcgccg cccattaggg gccagatcag
     6961 atgcagttca aatatcacag gcctgctcct gacacgcgac ggaggcccag aggacaacaa
     7021 gacagaggtg tttaggcccg gcggcggcga catgcgagat aattggaggt ctgaattgta
     7081 caagtacaag gtggtgaaaa tcgaacccct gggagtggcg cctactaagg caaagcgccg
     7141 cgttgtgcag agagagaaga gggcagtggg aattggggcg gtttttcttg ggttcctggg
     7201 ggctgcaggt agcaccatgg gagcggccgc tatgacactg acagtgcaag ccagactcct
     7261 gctgtctggt attgtccaac agcaaaacaa tttgctccgc gcaatagaag cccagcagca
     7321 cctgctccaa ctgactgtgt ggggcattaa acaattgcag gctcgcgtgc tggcagtcga
     7381 acggtacctg agggatcaac agctcctggg catctggggc tgctccggga aactcatctg
     7441 tacgacagcg gtcccttgga atgccagctg gtccaacaaa agcctcaaca agatttggga
     7501 caatatgacg tggattgagt gggacaggga aattaacaat tatacctcaa tcatatattc
     7561 tctgattgaa gagtcccaga atcagcaaga gaagaatgag caggaattgt tggaacttga
     7621 taaatgggca agcctgtgga actggtttga cataaccaaa tggttgtggt atatcaaaat
     7681 cttcataatg atcgtcggcg gactcatcgg gctccgaatt gtgttctcag tcctcagcat
     7741 tgtgaatagg gtgcgccagg gctattctcc cctgagcttc cagacccatc tgccctctag
     7801 ccgaggacca gataggcctg gtgggatcga ggaagaaggc ggggaacggg acagggatag
     7861 gagtggtcct cttgttaacg ggttcctgtg ataactcgag gccggcaagg ccggatccag
     7921 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     7981 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     8041 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     8101 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg
     8161 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     8221 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     8281 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg
     8341 gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc
     8401 ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg
     8461 gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat
     8521 ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac
     8581 aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt
     8641 acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg
     8701 tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc
     8761 ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta
     8821 accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag
     8881 taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta
     8941 catggggtca ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa
     9001 gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt
     9061 ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa
     9121 caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg
     9181 tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat
     9241 gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac
     9301 tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg
     9361 atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta
     9421 cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt
     9481 gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga
     9541 caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc
     9601 cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc
     9661 taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct
     9721 gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt
     9781 acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg
     9841 ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata
     9901 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     9961 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
    10021 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10081 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta
    10141 gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat
    10201 atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat
    10261 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
    10321 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
    10381 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
    10441 atgctatcct catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta
    10501 tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc
    10561 tgctggttgc tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc
    10621 tgattgctca ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg
    10681 agctgagtga cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg
    10741 gtgacaagac agatggccag aaatacacca acagcacgca tgatgtctac tggggattta
    10801 ttctttagtg cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt
    10861 acatcactcc tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc
    10921 gtcatcaccc tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac
    10981 tccatcgtca aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat
    11041 aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac
    11101 catgaaataa cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc
    11161 cattccaaag gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt
    11221 tcctcgcctt aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac
    11281 ccaagttcct tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc
    11341 ttctgcaatg ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca
    11401 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
    11461 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
    11521 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
    11581 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
    11641 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
    11701 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
    11761 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc ca
//
