LOCUS       sequence-239536-       11796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711gp160-754*
FEATURES             Location/Qualifiers
     source          1..11796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(700..718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        786..890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(891..959,960..1751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1109..1128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1922..2510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2411..2430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2941..3086
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3129..4166
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4164..4183)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4208..4256
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4910..5289
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5290..5493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5444..5464
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5490..5514
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7808..7942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7832..7851)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7886..7905
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8085..8107)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8667..10456
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt ctccggacac
       61 catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt ccgcctcctc
      121 gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt tgaggtccac
      181 tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca tttccaggtc
      241 ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag acatctttat
      301 tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac agccccccca
      361 ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc cccatcctcc
      421 gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg ctgaacatcc
      481 tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg
      541 gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc accagacaga
      601 gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca gcttatcgat
      661 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
      721 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      841 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      901 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      961 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1021 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1081 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     1141 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1201 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1261 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1321 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1381 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     1441 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1501 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1561 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1621 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1681 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1741 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1801 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1861 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2041 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2521 acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc tgatggtcgt
     2581 catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga
     2641 gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc
     2701 ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga
     2761 tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     2821 aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc cgtggcccgt
     2881 tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt tagttctatg
     2941 atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg
     3001 gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc
     3061 gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat cccggggggc
     3121 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     3181 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     3241 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     3301 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     3361 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     3421 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     3481 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     3541 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     3601 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     3661 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     3721 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     3781 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     3841 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     3901 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     3961 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     4021 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     4081 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccgg
     4141 atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4201 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
     4261 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4321 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     4381 agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta
     4441 gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt
     4501 gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt ggatggcctg
     4561 ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct gccaaacacc
     4621 cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg accaattctc
     4681 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
     4741 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
     4801 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     4861 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     4921 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4981 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5041 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5101 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5161 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5221 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5281 attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca
     5341 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5401 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
     5461 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga
     5521 agctgggtac cagctgctag cgccaccatg cctatgggga gcctccaacc cctggctaca
     5581 ctctatttgc ttggtatgct cgtagccagt gtcttggcta atctctgggt cacagtatat
     5641 tatggtgtac ccgtatggaa ggaagctaag accaccctct tctgtgcgtc tgacgcgaag
     5701 ggctacgaca aggaagttca caatgtttgg gctactcacg catgtgttcc gactgatccc
     5761 aacccgcaag agatgcctct ggagaatgtt accgagaatt tcaatatgtg ggaaaatgac
     5821 atggtgaatc aaatgcacga agatgtgatc agtctgtggg atgagtcctt gaagccttgc
     5881 gtcaaactga cccctctgtg cgtaactttg aactgcacgg atgttaataa aaatgtttcc
     5941 tcttccgaca cggataacta caaagagact atgaaggagc ggaaaaattg cacgttcaat
     6001 atgacgacag agcttcgcga caagaaccag aagaaatatg cccttttcta taagctcgac
     6061 attgtcccac tggatgataa tgacaatgcg tcataccggc ttataaattg caatacgagc
     6121 acgctgactc aggcctgccc aaaggttagt tttgacccta taccgatcca ctactgtgcc
     6181 cctgcagggt acgcaattct caaatgcaag aataaaacct tcaatgggat agggccgtgt
     6241 aacaaggttt caaccgttca atgcacgcat ggaataaagc cggtggtatc tacacaactt
     6301 ttgcttaacg ggagtcttgc ggaagaggac attgttattc gctcagagaa cataaccgat
     6361 aatgcgaaaa caataatcgt acacctcaat gagtccgtgg agatcgtttg catccgccct
     6421 aataataaca ctcggaaatc tattaggata ggtccaggcc aaacctttta cgcgacgggt
     6481 gatatagttg gggacatccg ccaggcatac tgcaatatct cagaggggaa atggaataag
     6541 actctccagc gggtatctga gaagctggct gagcacttcc cgaacagtac aattaacttc
     6601 aatagctcaa gcgggggtga cctggagatt acgacgcatt ctttcaattg tggcggggaa
     6661 tttttctatt gcaatacgtc tggcctcttc aacggaacct atatgaacaa tgatacgaag
     6721 agcaatgaca caaagtctaa cagttcatca atcatcacga tcccatgtcg cattaagcaa
     6781 attatcaaca tgtggcaaga agtcggacgc gcggtatacg caccacccat cgccggtaac
     6841 attacttgta aatccaacat tacgggcatt cttctgacta gggatggtgg gagaggcgaa
     6901 gaagtgaaaa atgataccga gacatttagg ccaggagggg gcaacatgaa agacaattgg
     6961 aggagtgagc tttataaata caaagtagtt gaaatcaagc ccctcggagt ggcaccaacc
     7021 gccgccaagc gcagagtcgt cgagagagaa aagagggcag taggacttgg ggcggtgctc
     7081 cttggatttc ttggggcagc cgggagtacg atgggcgcag cttccatcac tttgacggtt
     7141 caggcgcgac aactcctctc tggaattgtt caacagcagt ctaacttgct ccgggccatt
     7201 gaggcgcagc agcacatgtt gcagctcacc gtgtggggta ttaagcagct ccaagctagg
     7261 gtacttgcca tcgagcggta tcttaaggat caacagctcc tcggaatttg ggggtgttcc
     7321 ggtaagctca tttgcacaac agcggtcccc tggaactcca gttggtctaa taagaatcaa
     7381 actgagatat gggacaagat gacttggatg caatgggata gagagatttc aaattatacg
     7441 gacactatat accgccttct tgaagatagc caaaaccaac aggaaaagaa tgagaaggac
     7501 cttttggaac ttgacaagtg gcagaacctt tggagttggt tcaacataac taactggctc
     7561 tggtacataa gaatattcat aatgattgta gggggcttga taggcttgcg aataatattc
     7621 gcggttctgt caatcgtaaa ccgcgtacga caaggatatt caccgttgtc ttttcagacc
     7681 ctcgcgccga atcctcgggg attggaccgc ctggggcgga ttgaagaaga gggagggaaa
     7741 gaggacagga acagatccat tcgacttgtc cacggttttc tttgataact cgaggccggc
     7801 aaggccggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     7861 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     7921 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     7981 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     8041 ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8101 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8161 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat
     8221 cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg
     8281 cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat
     8341 gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa
     8401 atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct
     8461 gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat
     8521 gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca
     8581 atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg
     8641 tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata
     8701 tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat
     8761 cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt
     8821 cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa
     8881 gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct
     8941 tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt
     9001 gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt
     9061 cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat
     9121 actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga
     9181 caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc aacacataat
     9241 cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt
     9301 gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc
     9361 ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg
     9421 cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt
     9481 cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct
     9541 gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac
     9601 cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat
     9661 ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca
     9721 tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc
     9781 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     9841 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     9901 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     9961 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa
    10021 tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa
    10081 tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
    10141 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
    10201 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
    10261 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
    10321 tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta
    10381 gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct
    10441 gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa
    10501 agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa
    10561 ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg
    10621 ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt
    10681 ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg
    10741 tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc acctccttca
    10801 tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag
    10861 ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga
    10921 gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga
    10981 gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg
    11041 ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga
    11101 atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg
    11161 aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg
    11221 agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga
    11281 tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca
    11341 gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg
    11401 tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca
    11461 gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat
    11521 gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct
    11581 ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct
    11641 cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg
    11701 cctccacctc ctgctcctgc cnncctcctg ctcctgcccc tcctcctgct cctgcccctc
    11761 ctgctcctgc ccctcccgct cctgctcctg ctcctg
//
