LOCUS       sequence-239537-        4791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Sox2 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1875_pGL4-UBCmin-luc2/Sox2_SRR2
FEATURES             Location/Qualifiers
     source          1..4791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    36..84
                     /note="synthetic polyadenylation signal"
     misc_feature    98..189
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     138..157
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             377..2029
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2073..2194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2084..2103)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2138..2157
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2903..2920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3074..3662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3154..3173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3862..4653,4654..4722))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta ttttcattac
       61 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
      121 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
      181 catttctctg gcctaactgg ccggtacctg agctcgctag cctcgaggat ggggcggtga
      241 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      301 atttgaattc ctcgactcta gaggatctgg cctcggcggc caagcttggc aatccggtac
      361 tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
      421 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
      481 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
      541 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
      601 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
      661 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
      721 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
      781 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
      841 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
      901 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
      961 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     1021 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     1081 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     1141 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     1201 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     1261 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     1321 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     1381 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
     1441 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
     1501 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
     1561 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
     1621 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
     1681 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
     1741 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
     1801 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
     1861 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
     1921 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
     1981 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag
     2041 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     2101 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2161 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2221 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2281 tggtaaaatc gataaggatc ctctgccctt cagccgagta ccggccgccg ccagttctct
     2341 cagcctctag gcctgtgtcg cctcttttgc aagaaagtaa tttctttctt aaagagctac
     2401 taataatgtt ctttggagga ttatgaaggt cctgttttct gtgtttatat ttcttctcgg
     2461 tcttttcatt tcagatgtag agttggttgt ttgattcttt attttcgttt ttagggtaag
     2521 gtactgggaa gggacattta ttcagttccc agtccaagct aggcaggttc ccctctaatt
     2581 aatgcagaga ctctaaaaga atttcccggg ctcgggcagc cattgtgatg catataggat
     2641 tattcacgtg gtaatgagca cagtcgacag ttcttgctca cacataggaa taaaagaaac
     2701 tttgaataaa gacccaaatg acgggcgtgg ggggagaggg gaggatattc ccggaactcc
     2761 agctcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2821 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2881 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2941 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3001 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3061 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3121 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3181 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3241 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3301 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3361 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3421 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3481 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3541 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3601 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3661 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3721 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3781 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca
     3841 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
     3901 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
     3961 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
     4021 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
     4081 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
     4141 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
     4201 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
     4261 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
     4321 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
     4381 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
     4441 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
     4501 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
     4561 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
     4621 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
     4681 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
     4741 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa g
//
