LOCUS       sequence-239538-        4965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Klf2 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA2626_pGL4-UBCmin-luc2/Klf2-D'
FEATURES             Location/Qualifiers
     source          1..4965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    48..96
                     /note="synthetic polyadenylation signal"
     misc_feature    110..201
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     150..169
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      383..392
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             389..2041
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2085..2206
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2096..2115)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2150..2169
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3089..3106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3260..3848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3340..3359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4048..4839,4840..4908))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
       61 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
      121 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
      181 caggtgccag aacatttctc tggcctaact ggccggtacc tgagctcgct agcctcgagg
      241 atggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc
      301 gtcgcagccg ggatttgaat tcctcgactc tagaggatct ggcctcggcg gccaagcttg
      361 gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc
      421 agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa
      481 gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat
      541 tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg
      601 gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc
      661 cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga
      721 gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa
      781 agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat
      841 catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca
      901 tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac
      961 catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc
     1021 gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat
     1081 catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac
     1141 cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga
     1201 gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact
     1261 atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga
     1321 gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt
     1381 ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat
     1441 cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc
     1501 taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg
     1561 cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct
     1621 catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca
     1681 cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc
     1741 agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg
     1801 cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa
     1861 aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa
     1921 gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga
     1981 cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta
     2041 ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     2101 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2161 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2221 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2281 cctctacaaa tgtggtaaaa tcgataagga tctgcatccc aagcaccaag caggtgacac
     2341 acccatgtca tcccagtact agggaagcag gcaggaggat cctgagttca aaagtatcca
     2401 cagctatggt gagctatggc taccctttgc tacatgactt tttttttttc ctaaagttta
     2461 aagtcataac caacatgttc ctaaaagtgg tcaagcctcg cttgtccagg aaaaagagtt
     2521 ttgaaacata ctcacaagca ccactgtctt gctttaagct acagtagtag atcacaacaa
     2581 accagactga actgggctac gaccttggga agaatctgca cttaacctgg ctagtgcagt
     2641 tgacatgaca aagacagggc cttcagtcta gagcctcaca caaaagaggc caccaacaga
     2701 agccaggagg ccaatcaagc ccagaatttt tttttattgt gtttctagtt tgcaaagagt
     2761 gtgactttga aatgcctaat ctgcatcttt tccctaaaac caaaaaaaca aacaaaacca
     2821 actcggaata acaacaacaa caaaaaagct tgtagaaagt ggtatggggt gtggcagcct
     2881 gttcctccag cttcagcctc cggattgcca ggaactgggg actgaagccg ccttcttggt
     2941 cccctatcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     3001 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     3061 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3121 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3181 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3421 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3661 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     4021 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     4081 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     4141 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     4201 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     4261 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     4321 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     4381 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     4441 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     4501 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     4561 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     4621 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     4681 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     4741 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     4801 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     4861 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4921 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctca
//
