LOCUS       sequence-239539-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     109..131
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     123..140
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     124..140
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     147..166
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        147..165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   184..218
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      4225..4234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4231..4233,4234..4236,4237..4950)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4231..4233,4234..4236,4237..4947)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4276..4297)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4537..4556)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4884..4905
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4991..5565,5566..5568,5569..5577)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5158..5175)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5385..5404
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5578..6177
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5578..5597)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6074..6094
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6258..6275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6264..6488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6535..6551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6559..6575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6583..6589,6590..6607,6608..6613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6628..6649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6876..6938
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6962..6980)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7001..7017)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7025..7041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7049..7055,7056..7073,7074..7079))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7094..7115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7232..7249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7403..7991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7483..7502)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8162..8953,8954..9022))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8785..8804
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9023..9127)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9153..9608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9290..9311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9502..9521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
       61 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      121 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacct
      181 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
      241 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
      301 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
      361 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
      421 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
      481 gttatagata tcgtcatcat aatttaaaca agcaaaacca aattaagggc cagctcattc
      541 ctcccactca tgatctatag atctcacgcg tagggagacc cagcctggat gctaacacga
      601 gtgatttccc tgctctagtc tagtgtcctc cgtgagtcca tttaactgat cacccagtct
      661 gtgaggaggt ggctgaactc acagtaagaa agctgtgggg gtcaacgcct attgtttgtt
      721 tgttttgttt tagacaaggt ctcctgctga ggctggctca agctggcctg gaggactctt
      781 gtgtttaagg ctggccttaa attctcttta aaagaaaatc atgtgtatat atgtgtgtcc
      841 atgtaactgc agatgaccac agcagccaag agatttctgt tctcctagct gtaaaccacc
      901 caatatgggt gctaggaaca gaattttaaa agggtcctct gaaagagcaa tgtacactca
      961 aatgctgagt tctttcccag cccctagcct tggacctttg ttcttatcac ttccaacgcc
     1021 caagggcagg cattataggt gtggcattcc gcatctggct tcccaggata ccttttcatg
     1081 ctggtggacc atctctggct ggggacgtgt gggcttctct gctgtctttg gttctccaga
     1141 cagaactccg agacagatct tgacttggtt ctaaaataca ggtggtttgt ggcaagttaa
     1201 cgaattttag ctcaaatttg gggtatttaa gataccatgg tgactctttt ttgtttgttt
     1261 ttctttcttt cttttttttt tttaaaaaga tttatttatt attgtacgta agtacttcag
     1321 acaccagaag agggtgacag attttttttt tttttcgaga caggatttct ctgtgaagcc
     1381 ctggctgtcc tggaactcac tctgtagacc aggctggcct ggaactcaga aatccacctg
     1441 cctctgcctc ccaagtgctg ggattaaagc catgagttgg gaccgcaccc ggcccaaagt
     1501 gactcttaaa gggggcagag tggcacatat tttcaatcct agcactcagg aagcaaaggc
     1561 tggtggatct ctgtgagttc aaggccagcc tggtctacag agtgagttcc aagccatcta
     1621 aggctatgta gggaaccctt gaatcaaacc caaaagttag tctgatgatt ttctaagacc
     1681 caggaggcaa gaaactggat cagatgagcc aacaggtctg ctgtcccatc tccagggcca
     1741 ccaggctcac agctcgggac caggctaggg cacatctgtt tcaagctagt tctaagaaga
     1801 cttgggactt cagacaaagt tgctgttaag gactgtatta tactctaggc acgcttaggg
     1861 ctaacctggt tgcaaagcca gtcactaggc agttaaagga ctcagaatat gtctcttgtc
     1921 ctggccagtg agtcaccaaa agagaaatca caatccataa gacaaggttg gtattgaata
     1981 cagacaggac tgctgggctg caggcatact tgaactgtgg tggagagtgc tgtctaggcc
     2041 ttagaggctg gccctgggag gaactgggtg tggggaggtt gtagcccgac cctgcccctc
     2101 cccccaggga ggttgagagt tctgggcaga cggcagatgc ataacaaagg tgcatgatag
     2161 ctctgccctg ggggcagaga agatggttgg ggaggggtcc ctctcgtcct agcccttcct
     2221 taatctgcta ttgaggaagc tttgtgaact tggcggcttc caagtcgctg cctttattta
     2281 ggtcttccaa ctaacctatg gcactgttcc acaatgaatg tatagaaatt gggaggtgag
     2341 catgacagag tggaggaaac ggaagattca tggagagggc cagagagatg gcccctcagc
     2401 caccctgggg gatgacttgg acccatgtgg tagaaggagg ggacttccac acatgtgcta
     2461 tgtgtagctg tgtgtaggta catacacacc cttaaaataa aacgcaattt ttttttcaaa
     2521 gtctcagggt gaatttggtg aagtcgatga agctgaggca ggagaattat caggagttca
     2581 agggcagctt gttttataga gaaaggttcc atctctacct gatgaagact accatcaaga
     2641 gacacccccg ccccccaggg cacctagagc cactgaccct agccaacagc tcaggcgggc
     2701 tgggcccagg ctcagaactc tgtcctggct atgtacactg tggggtgctc tgggcttttt
     2761 gaggctgtgt gattcaccct ggggccttcg ttcagagcat ggtgtaggag cagacagaca
     2821 aacaccatcc cttgcagaca ggcactctga gggctattct cttgcaaaga taactaagca
     2881 ccaggccagt aatgggatcc tcagactggg cccagaaaac cactctaggg aagttcaggg
     2941 taggctctct gcaccccctc ctcctaatcc cgtctcctta gtgtctttcc gccagcacag
     3001 gaatggggga ggggtgggtg acgaggatga acaccggagt ccctggagga agggaagcag
     3061 ggtatctcca tctgaggctc tgtctttgag gagaggtgga gagctgggga agtcttgtgt
     3121 gaggggattg gggctcagga gggggttggg gagcaggaag ttgtccccag gggagccatc
     3181 ctggcccatt caagggttga gtacttgttt agggttagag ctgccccctc tggggaccag
     3241 gattgtccag ccaaggccat tgtcctgccc ccttccccca gtccctccca ggcccctttg
     3301 aacctgaagt cagatatttc ttctctctac ccacctccca cccgttgggt ttctccaccc
     3361 aggaactagg ctggaagcct gggatgagga ggtgggggga gggagaactg agaatcttga
     3421 ggaaagaggc cccggcctta actgtgaggg gatggagcct gggtgcaggt cttatggggg
     3481 ttggggggtg gttagtgtct aatctaccaa cctggacaac acaagatgga atactgtgct
     3541 ctgaaaacgc agagccagca cttctctggg gtctctgggg acatatctgg ttggggctcg
     3601 gggtcccatg gtgtagagcc tctaaactct ggaggactgg aggtgcaatg gctgtcttgt
     3661 cctggccttg gacatgggct gaaatactgg gttcacccat atctaggact ctagacgggt
     3721 gggtaagcaa gaactgagga gtggccccag aaataattgg cacacgaaca ttcaatggat
     3781 gttttaggct ctccagagga tggctgagtg ggctgtaagg acaggccgag agggtgcagt
     3841 gccaacaggc tttgtggtgc gatggggcat ccgagcaact ggtttgtgag gtgtccggtg
     3901 acccaaggca ggggtgagag gaccttgaag gttgaaaatg aaggcctcct ggggtcccgt
     3961 cctaagggtt gtcctgtcca gacgtcccca acctccgtct ggaagacaca ggcagatagc
     4021 gctcgcctca gtttctccca cccccacagc tctgctcctc cacccaccca gggggcgggg
     4081 ccagaggtca aggctagagg gtgggattgg ggagactagt gagggggggc ccggtacccg
     4141 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     4201 gcagccggga tttgaattcc tcgagccacc atggtgagca agggcgagga gctgttcacc
     4261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4441 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4681 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4921 actctcggca tggacgagct gtacaagtaa gcggccgctc gataagcttg atatcgaatt
     4981 ccgccccccc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     5041 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5101 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5161 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     5221 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     5281 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     5341 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     5401 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     5461 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     5521 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     5581 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5641 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5701 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5761 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     5821 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     5881 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     5941 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6001 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6061 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6121 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     6181 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgatgatc
     6241 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6301 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6361 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6421 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6481 ctctatggct tctgaggcgg aaagaacctg cagcccaagc ttggcgtaat catggtcata
     6541 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6601 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6661 ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccttt gttactttat
     6721 agaagaaatt ttgagttttt gttttttttt aataaataaa taaacataaa taaattgttt
     6781 gttgaattta ttattagtat gtaagtgtaa atataataaa acttaatatc tattcaaatt
     6841 aataaataaa cctcgatata cagaccgata aaacacatgc gtcaatttta cgcatgatta
     6901 tctttaacgt acgtcacaat atgattatct ttctagggtt aaagagctcc agcttttgtt
     6961 ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7021 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7081 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7141 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7201 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7801 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9121 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     9181 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     9241 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     9301 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     9361 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     9421 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     9481 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     9541 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     9601 gggcgcgtcc cattcgccat tcaggc
//
