LOCUS       sequence-239541-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human FLAG-MYO1D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   McAlpine W, Wang KW, Choi JH, San Miguel M, McAlpine SG, Russell J, 
            Ludwig S, Li X, Tang M, Zhan X, Choi M, Wang T, Bu CH, Murray AR, 
            Moresco EMY, Turer EE, Beutler B
  TITLE     The class I myosin MYO1D binds to lipid and protects against 
            colitis.
  JOURNAL   Dis Model Mech. 2018 Sep 27;11(9). pii: 11/9/dmm035923. doi: 
            10.1242/dmm.035923.
  PUBMED    30279225
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dis Model 
            Mech."; date: "2018-09-27"; volume: "11(9). pii: 11/9/dmm035923. 
            doi"; pages: " 10.1242/dmm.035923"
COMMENT     Sequence Label: pcDNA-FLAG-MYO1D
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttag agctccttaa ggttaacgcc accatggact
      121 acaaagacga tgacgacaag tctagagaat tcggatccgc ggagcaggag agcctggaat
      181 tcggcaaggc agacttcgtg ctgatggaca ccgtctccat gcccgagttc atggccaacc
      241 tcaggctcag atttgaaaaa gggcgcatct atacgttcat tggagaagtc gtcgtttctg
      301 tgaaccctta caagttgttg aacatctatg gaagagacac aattgagcag tataaaggcc
      361 gtgagctgta tgagagaccg cctcaccttt ttgctattgc ggatgctgct tacaaggcta
      421 tgaagaggcg atcaaaagac acttgtattg tgatatcagg ggaaagtgga gctggtaaaa
      481 cggaagccag taagtacatt
//
