LOCUS       sequence-239543-       11797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711-QES.c11-754*
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(721..739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(912..980,981..1772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1130..1149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2432..2451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2962..3107
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3150..4187
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4185..4204)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4229..4277
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4931..5310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5311..5514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5465..5485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5511..5535
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7829..7963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7853..7872)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7907..7926
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8106..8128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8688..10477
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
       61 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
      121 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
      181 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
      241 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
      301 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
      361 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
      421 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
      481 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
      541 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
      601 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
      661 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      961 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1021 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1081 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1141 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1201 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1261 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1321 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1381 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1441 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1501 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1561 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1621 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1681 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1741 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1801 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2581 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     2641 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     2701 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     2761 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2821 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     2881 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     2941 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     3001 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     3061 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     3121 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     3181 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3241 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3301 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3361 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     3421 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3481 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     3541 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3601 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3661 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3721 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3781 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3841 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3901 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     3961 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     4021 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4081 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4141 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
     4201 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4441 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     4501 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     4561 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     4621 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
     4681 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
     4741 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
     4801 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     4861 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     4921 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4981 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5041 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5101 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5161 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     5221 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     5281 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     5341 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5401 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     5461 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     5521 gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat gcctatgggg
     5581 agcctccaac ccctggctac actctatttg cttggtatgc tcgtagccag tgtcttggct
     5641 aatctctggg tcacagtata ttatggtgta cccgtatgga aggaagctaa gaccaccctc
     5701 ttctgtgcgt ctgacgcgaa gggctacgac aaggaagttc acaatgtttg ggctactcac
     5761 gcatgtgttc cgactgatcc caacccgcaa gagatgcctc tggagaatgt taccgagaat
     5821 ttcaatatgt gggaaaatga catggtgaat caaatgcacg aagatgtgat cagtctgtgg
     5881 gatgagtcct tgaagccttg cgtcaaactg acccctctgt gcgtaacttt gaactgcacg
     5941 gatgttaata aaaatgtttc ctcttccgac acggataact acaaagagac tatgaaggag
     6001 cggaaaaatt gcacgttcaa tatgacgaca gagcttcgcg acaagaacca gaagaaatat
     6061 gcccttttct ataagctcga cctggtccca ctggatgata atgacaatgc gtcataccgg
     6121 cttataaatt gcaatacgag cacgctgact caggcctgcc caaagatgag ttttgaccct
     6181 ataccgatcc actactgtgc ccctgcaggg tacgcaattc tcaaatgcaa gaataaaacc
     6241 ttcaatggga tagggccgtg taacaaggtt tcaaccgttc aatgcacgca tggaataaag
     6301 ccgaccgtat ctacacaact tttgcttaac gggagtcttg cggaagagga cattgttatt
     6361 cgctcagaga acataaccga taatgcgaaa acaataatcg tacacctcaa tgagtccgtg
     6421 gagatcgttt gcatccgccc taataataac actcggaaat ctattaggat aggtccaggc
     6481 caaacctttt acgcgacggg tgatatagtt ggggacatcc gccaggcata ctgcaatatc
     6541 tcagagggga aatggaataa gactctccag cgggtatctg agaagctggc tgagcacttc
     6601 ccgaacagta caattaactt caatagctca agcgggggtg acctggagat tacgacgcat
     6661 tctttcaatt gtggcgggga atggttctat tgcaatacgt ctggcctctt caacggaacc
     6721 tatatgaaca atgatacgaa gagcaatgac acaaagtcta acagttcatc aatcatcacg
     6781 atcccatgtc gcattaagca aattatcaac atgtggcaag aagtcggacg cgcggtatac
     6841 gcaccaccca tcgccggtaa cattacttgt aaatccaaca ttacgggcat tcttctgact
     6901 agggatggtg ggagaggcga agaagtgaaa aatgataccg agacatttag gccaggaggg
     6961 ggcaacatga aagacaattg gaggagtgag ctttataaat acaaagtagt tgaaatcaag
     7021 cccctcggag tggcaccaac cgccgccaag cgcagagtcg tcgagagaga aaagagggca
     7081 gtaggacttg gggcggtgct ccttggattt cttggggcag ccgggagtac gatgggcgca
     7141 gcttccatca ctttgacggt tcaggcgcga caactcctct ctggaattgt tcaacagcag
     7201 tctaacttgc tccgggccat tgaggcgcag cagcacatgt tgcagctcac cgtgtggggt
     7261 attaagcagc tccaagctag ggtacttgcc atcgagcggt atcttaagga tcaacagctc
     7321 ctcggaattt gggggtgttc cggtaagctt atttgcacaa cagcggtccc ctggaactcc
     7381 agttggtcta ataagaatca aactgagata tgggacaaga tgacttggat gcaatgggat
     7441 agagagattt caaattatac ggacactata taccgccttc ttgaagatag ccaaaaccaa
     7501 caggaaaaga atgagaagga ccttttggaa cttgacaagt ggcagaacct ttggagttgg
     7561 ttcaacataa ctaactggct ctggtacata agaatattca taatgattgt agggggcttg
     7621 ataggcttgc gaataatatt cgcggttctg tcaatcgtaa accgcgtacg acaaggatat
     7681 tcaccgttgt cttttcagac cctcgcgccg aatcctcggg gattggaccg cctggggcgg
     7741 attgaagaag agggagggaa agaggacagg aacagatcca ttcgacttgt ccacggtttt
     7801 ctttgataac tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt
     7861 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7921 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     7981 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     8041 ctacaaatgt ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt
     8101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     8161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     8221 atgaggtcga ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     8281 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     8341 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     8401 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     8461 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     8521 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     8581 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     8641 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     8701 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     8761 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     8821 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     8881 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     8941 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     9001 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     9061 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     9121 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     9181 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     9241 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     9301 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     9361 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     9421 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     9481 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     9541 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     9601 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     9661 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     9721 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     9781 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     9841 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     9901 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     9961 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
    10021 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
    10081 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
    10141 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
    10201 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
    10261 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    10321 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc
    10381 agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc
    10441 aagggggcgt gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg
    10501 aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc
    10561 taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt
    10621 atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata
    10681 caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg
    10741 cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc
    10801 tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct
    10861 tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc
    10921 accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc
    10981 aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc
    11041 cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat
    11101 ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag
    11161 gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt
    11221 tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg
    11281 ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca
    11341 tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc
    11401 tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca
    11461 aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc
    11521 ggtggagacc cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt
    11581 ccacgacctc tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta
    11641 ccccggcggc ctccactacc tcctcgaccc cggcctccac tacctcctcg accccggcct
    11701 ccactgcctc ctcgaccccg gcctccacct cctgctcctg ccnnncctcc tgctcctgcc
    11761 cctcctcctg ctcctgcccc tcctgctcct gcccctc
//
