LOCUS       sequence-239546-        4841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter driven by the Tbx3 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA2628_pGL4-UBCmin-luc2/Tbx3-B'
FEATURES             Location/Qualifiers
     source          1..4841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    18..66
                     /note="synthetic polyadenylation signal"
     misc_feature    80..171
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     120..139
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      353..362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             359..2011
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2055..2176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2066..2085)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2120..2139
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2935..2952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3106..3694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3186..3205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3894..4685,4686..4754))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggtattggac aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
       61 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
      121 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tggcctaact
      181 ggccggtacc tgagctcgct agcctcgagg atggggcggt gaacgccgat gattatataa
      241 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgaat tcctcgactc
      301 tagaggatct ggcctcggcg gccaagcttg gcaatccggt actgttggta aagccaccat
      361 ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac
      421 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
      481 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
      541 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
      601 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
      661 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
      721 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
      781 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
      841 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
      901 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
      961 taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca
     1021 tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt
     1081 gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg
     1141 ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa
     1201 gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat
     1261 cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa
     1321 ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg
     1381 cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc
     1441 agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac
     1501 actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta
     1561 cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg
     1621 cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct
     1681 gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca
     1741 ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc
     1801 cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta
     1861 tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga
     1921 ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa
     1981 ggccaagaag ggcggcaaga tcgccgtgta ataattctag agtcggggcg gccggccgct
     2041 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2101 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2161 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2221 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     2281 tctacctacc caaggctcct tgcatctgag gcttgtttta attttttatt tgttttggtt
     2341 tgatttttga gagggttcct ccacctagcc cagactcctt tcaaactgag gatcctcctg
     2401 cctcagcctc tctgacactg gaattgcaac atgtgtctac cacaccccgt tgtacctcag
     2461 gctgtgtggt gtgtatgcat gtgagtgtgc gtgtatgagg gtgcgacaga gcatgtgtct
     2521 gcacttaggg cctgaaggtg acaagggaca cattttcatc ctccctaaag actcaattct
     2581 ctgccctttg gcaaggtgag aagatcctgg aatttgttgt ttaaaataag aaaagaaagt
     2641 agattggagt ttcccggact taactctttt tggagaaatc actttatctt ttttaattgt
     2701 cccgttttta tctccacccc tccagttccc aaaattagca atagatatgc taagagaatt
     2761 caacaggata atagaagtta gccatgcccg cagggtcgac cgatgccctt gagagccttc
     2821 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     2881 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     2941 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3001 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3061 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3121 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3181 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3241 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3301 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3361 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3421 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3481 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3541 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3601 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3661 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3721 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3781 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3841 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     3901 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     3961 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     4021 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     4081 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     4141 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     4201 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     4261 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     4321 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     4381 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     4441 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     4501 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     4561 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     4621 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     4681 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     4741 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     4801 tacgtctctc aaggataagt aagtaatatt aaggtacggg a
//
