LOCUS       sequence-239556-        4088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible IPTG inducible integration vector for 
            Streptococcus pneumoniae (pPEPZ Backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pASR102
FEATURES             Location/Qualifiers
     source          1..4088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..72
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      88..115
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    212..231
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     rep_origin      1362..1950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1851..1870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3116..3649)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
       61 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      121 agaattcact agtaattgtg aggcggataa caattctcac tcattctaca gtttattctt
      181 gacattgcac tgtccccctg gtataataac taattgtgag cgctcacaat taagatctaa
      241 ataaataagg aggaaaaata atgccggatc cctccagtaa ctcgagaaaa aaaaaccgcg
      301 cccctgacag ggcgcggttt ttttttcgta atttattact gaacaagagg gcaatcatat
      361 ctggaataat atgcttaaaa agagaaggaa aattggtgca aattcatttt cagactatct
      421 tcgtggaagt attcatcaaa atagacaaaa ataaatttgg ataaatcgaa ctcactattc
      481 aggaggcata tgagcaattc gaaaaagaaa agtgtcaaat tgagcctata ggagtagaag
      541 tgaaatagta agtcctgcat agtggatgag agaaaagttc tccttgaagt tttcctgaac
      601 tatcagtcgc atgtcaaacg atatgtaggg taatgtgaga ggggatagcg agtagttttt
      661 ggttatttta tcaaaaaact tatattttat tataccgaat gataaaatat aataaaaatg
      721 atagaataag gaaaaaacat gaatgtcaaa aagataatgt caatttttca atccttttat
      781 gttgatgtca gtattgagga actgactttg actttaccaa tcagttttgt aaaaaggttt
      841 gagtatactc aaatgacttt tcataaggaa tcatttttat tgattaaaga aaagagaagg
      901 gggagtttga gttcatttgt tactcaggct cgcactatgg gtgaaaaagc caatatggat
      961 gttgttttgg tgttttcgaa gttatcagac agtgaaaaaa agcaattact tcaagctaga
     1021 gttccgtttg tagactttaa gggaaacctc ttcttccctc cattgggact agtactcaat
     1081 gcgaatgata ctgaagtccc taaggaatta acacctagcg aacaattaac gtggattgcc
     1141 tttttattga caaaaggtca aaaagtagta gatgttgatt tgctttcaca agtcactgga
     1201 cttccaaact caacaattta taggtgtttg aggactttta aagctttata ttggttaaac
     1261 aagcaaaata agctttacac atatacggtg tcaaagaaag aattattctt aaaatccgtg
     1321 tcatgttggt accaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctgg tagcggtggt ttgtttgccg
     1441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1501 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1981 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccg cggccgccaa
     2041 taaattgctt ccttgttttg tcataatttt tttatttaaa aaattatgac aaaagtgtgc
     2101 tattcttttt atgagaggtg tatgaatatg ataaatgtat gtgataaatg tatgtgatgt
     2161 tggaaaaaga ataaaagaac ttagaatatc ttcaaatctt actcaagata agattgctga
     2221 atatttgtct ttatatttgt ctttgaatca aagcatgatt gccaaaatgg aaaaaggtga
     2281 aaggaatatc acgaatggat ttaagtaata aagcttcaaa tcttagaaaa aagttgggag
     2341 ctgatggtga agcgccgata gatattttta aattggtaca aaagatagaa aatttgacgc
     2401 tggtatttta tggactcgga aagaatatta gcggagtctg ttataaagga actcagttca
     2461 gtctcattgc agtcaattca gacatgtcat taggaaggta aagattttct ttagcacatg
     2521 aactgtatca tctttattat gatgaggtga agaagagttc agtcagtctt atcttgattg
     2581 gtgaaggaga tgaaactgaa agaaaagcgg atcagcttgc ttcttatttt ttaattttcc
     2641 catcttcact gtataggatg gttgaggaaa tcagagaaaa tgccaataga actcatcttg
     2701 aagtagaaga tattataaaa ttgggtcagt tttatggtat cagtcataaa gctatgttat
     2761 atagattgag gaatgatgga taccttgatg cagaagaaat taaaaatatg gatattagtg
     2821 ttatagagac agcttcaaga ttaggctatg atacaagttt atatcgtcct ttgtcagaaa
     2881 gtaaaaagga aatggtatta ggataatata ttaattcaac tgaacaactt ttagaaaata
     2941 acagaatttc gcaagggaag tatgaggaac tgttactaga tgctttcaga tatgatattg
     3001 tatatgggct agatgaagag gggggagttg tcgttgaaaa gcttctagca aaaaactgga
     3061 cggagtcccg tccagaaaag taagcacttg ttattgcaat aaaattagct tattattagg
     3121 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     3181 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     3241 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     3301 gctcgccgga gactgcgaga tcatagatat agatttcact acgcggctgc tcaaacttgg
     3361 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     3421 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     3481 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     3541 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     3601 ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatt aattttcctc
     3661 ctacatgatt tcacctcgtt gattatgttc atataaagtt tatattgata ctcaatttac
     3721 ttaccctaga ttggacatat acttaaatta ctgttcaata aagctgaccg ttagcgttta
     3781 agtacatcct ttcacaattt gtctacagaa cagtactggc tattatatca gccttcttat
     3841 tcaatgtcaa gaataaactg ttctatgtaa acctattcat tgttttgcat gcggcaaagc
     3901 actcaaaagt ttaccttatg ggtgcttttt tcgtgctttt ttgaaaagac aaaaaaaaga
     3961 accttgccaa gcaagattct tttgataggg tggtctccga tcgtgtactg cagaaaacta
     4021 gtcgcaaaaa accccgcttc ggcggggttt tttcgctcta gacggtgatc aacacgctag
     4081 ccaggcat
//
