LOCUS       sequence-239567-        8957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac-based reporter (Puro) driven by the Pou5f1 enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, 
            Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR
  TITLE     Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive 
            Pluripotency.
  JOURNAL   Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 
            10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
  PUBMED    29910149
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2018.05.020"; journalName: "Cell Stem Cell"; date: 
            "2018-08-2- 2"; volume: "23"; issue: "2"; pages: "266-275.e6"
COMMENT     Sequence Label: KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP
FEATURES             Location/Qualifiers
     source          1..8957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     116..133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     117..133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     140..159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        140..158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   177..211
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             4246..4272
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4315..4889,4890..4892,4893..4901)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4482..4499)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4709..4728
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4902..5501
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4902..4921)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5398..5418
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5582..5599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5588..5812
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5859..5875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5883..5899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5907..5913,5914..5931,5932..5937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5952..5973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   6200..6262
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6286..6304)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6325..6341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6349..6365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6373..6379,6380..6397,6398..6403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6418..6439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6556..6573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6727..7315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6807..6826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7486..8277,8278..8346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8109..8128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8347..8451)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8477..8932)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8614..8635)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8826..8845
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
       61 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      121 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggta ccttaaccct
      181 agaaagatag tctgcgtaaa attgacgcat gcattcttga aatattgctc tctctttcta
      241 aatagcgcga atccgtcgct gtgcatttag gacatctcag tcgccgcttg gagctcccgt
      301 gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga actataacga ccgcgtgagt
      361 caaaatgacg catgattatc ttttacgtga cttttaagat ttaactcata cgataattat
      421 attgttattt catgttctac ttacgtgata acttattata tatatatttt cttgttatag
      481 atatcgtcat cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac
      541 tcatgatcta tagatctcac gcgtagggag acccagcctg gatgctaaca cgagtgattt
      601 ccctgctcta gtctagtgtc ctccgtgagt ccatttaact gatcacccag tctgtgagga
      661 ggtggctgaa ctcacagtaa gaaagctgtg ggggtcaacg cctattgttt gtttgttttg
      721 ttttagacaa ggtctcctgc tgaggctggc tcaagctggc ctggaggact cttgtgttta
      781 aggctggcct taaattctct ttaaaagaaa atcatgtgta tatatgtgtg tccatgtaac
      841 tgcagatgac cacagcagcc aagagatttc tgttctccta gctgtaaacc acccaatatg
      901 ggtgctagga acagaatttt aaaagggtcc tctgaaagag caatgtacac tcaaatgctg
      961 agttctttcc cagcccctag ccttggacct ttgttcttat cacttccaac gcccaagggc
     1021 aggcattata ggtgtggcat tccgcatctg gcttcccagg ataccttttc atgctggtgg
     1081 accatctctg gctggggacg tgtgggcttc tctgctgtct ttggttctcc agacagaact
     1141 ccgagacaga tcttgacttg gttctaaaat acaggtggtt tgtggcaagt taacgaattt
     1201 tagctcaaat ttggggtatt taagatacca tggtgactct tttttgtttg tttttctttc
     1261 tttctttttt ttttttaaaa agatttattt attattgtac gtaagtactt cagacaccag
     1321 aagagggtga cagatttttt ttttttttcg agacaggatt tctctgtgaa gccctggctg
     1381 tcctggaact cactctgtag accaggctgg cctggaactc agaaatccac ctgcctctgc
     1441 ctcccaagtg ctgggattaa agccatgagt tgggaccgca cccggcccaa agtgactctt
     1501 aaagggggca gagtggcaca tattttcaat cctagcactc aggaagcaaa ggctggtgga
     1561 tctctgtgag ttcaaggcca gcctggtcta cagagtgagt tccaagccat ctaaggctat
     1621 gtagggaacc cttgaatcaa acccaaaagt tagtctgatg attttctaag acccaggagg
     1681 caagaaactg gatcagatga gccaacaggt ctgctgtccc atctccaggg ccaccaggct
     1741 cacagctcgg gaccaggcta gggcacatct gtttcaagct agttctaaga agacttggga
     1801 cttcagacaa agttgctgtt aaggactgta ttatactcta ggcacgctta gggctaacct
     1861 ggttgcaaag ccagtcacta ggcagttaaa ggactcagaa tatgtctctt gtcctggcca
     1921 gtgagtcacc aaaagagaaa tcacaatcca taagacaagg ttggtattga atacagacag
     1981 gactgctggg ctgcaggcat acttgaactg tggtggagag tgctgtctag gccttagagg
     2041 ctggccctgg gaggaactgg gtgtggggag gttgtagccc gaccctgccc ctccccccag
     2101 ggaggttgag agttctgggc agacggcaga tgcataacaa aggtgcatga tagctctgcc
     2161 ctgggggcag agaagatggt tggggagggg tccctctcgt cctagccctt ccttaatctg
     2221 ctattgagga agctttgtga acttggcggc ttccaagtcg ctgcctttat ttaggtcttc
     2281 caactaacct atggcactgt tccacaatga atgtatagaa attgggaggt gagcatgaca
     2341 gagtggagga aacggaagat tcatggagag ggccagagag atggcccctc agccaccctg
     2401 ggggatgact tggacccatg tggtagaagg aggggacttc cacacatgtg ctatgtgtag
     2461 ctgtgtgtag gtacatacac acccttaaaa taaaacgcaa tttttttttc aaagtctcag
     2521 ggtgaatttg gtgaagtcga tgaagctgag gcaggagaat tatcaggagt tcaagggcag
     2581 cttgttttat agagaaaggt tccatctcta cctgatgaag actaccatca agagacaccc
     2641 ccgcccccca gggcacctag agccactgac cctagccaac agctcaggcg ggctgggccc
     2701 aggctcagaa ctctgtcctg gctatgtaca ctgtggggtg ctctgggctt tttgaggctg
     2761 tgtgattcac cctggggcct tcgttcagag catggtgtag gagcagacag acaaacacca
     2821 tcccttgcag acaggcactc tgagggctat tctcttgcaa agataactaa gcaccaggcc
     2881 agtaatggga tcctcagact gggcccagaa aaccactcta gggaagttca gggtaggctc
     2941 tctgcacccc ctcctcctaa tcccgtctcc ttagtgtctt tccgccagca caggaatggg
     3001 ggaggggtgg gtgacgagga tgaacaccgg agtccctgga ggaagggaag cagggtatct
     3061 ccatctgagg ctctgtcttt gaggagaggt ggagagctgg ggaagtcttg tgtgagggga
     3121 ttggggctca ggagggggtt ggggagcagg aagttgtccc caggggagcc atcctggccc
     3181 attcaagggt tgagtacttg tttagggtta gagctgcccc ctctggggac caggattgtc
     3241 cagccaaggc cattgtcctg cccccttccc ccagtccctc ccaggcccct ttgaacctga
     3301 agtcagatat ttcttctctc tacccacctc ccacccgttg ggtttctcca cccaggaact
     3361 aggctggaag cctgggatga ggaggtgggg ggagggagaa ctgagaatct tgaggaaaga
     3421 ggccccggcc ttaactgtga ggggatggag cctgggtgca ggtcttatgg gggttggggg
     3481 gtggttagtg tctaatctac caacctggac aacacaagat ggaatactgt gctctgaaaa
     3541 cgcagagcca gcacttctct ggggtctctg gggacatatc tggttggggc tcggggtccc
     3601 atggtgtaga gcctctaaac tctggaggac tggaggtgca atggctgtct tgtcctggcc
     3661 ttggacatgg gctgaaatac tgggttcacc catatctagg actctagacg ggtgggtaag
     3721 caagaactga ggagtggccc cagaaataat tggcacacga acattcaatg gatgttttag
     3781 gctctccaga ggatggctga gtgggctgta aggacaggcc gagagggtgc agtgccaaca
     3841 ggctttgtgg tgcgatgggg catccgagca actggtttgt gaggtgtccg gtgacccaag
     3901 gcaggggtga gaggaccttg aaggttgaaa atgaaggcct cctggggtcc cgtcctaagg
     3961 gttgtcctgt ccagacgtcc ccaacctccg tctggaagac acaggcagat agcgctcgcc
     4021 tcagtttctc ccacccccac agctctgctc ctccacccac ccagggggcg gggccagagg
     4081 tcaaggctag agggtgggat tggggagact agtgaggggg ggcccggtac ccggggcggt
     4141 gaacgccgat gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg
     4201 ggatttgaat tcctcgagat ctcacgcgtg actagtgcca tggcctaccc atacgacgtg
     4261 ccagactacg cctagcggcc gctcgataag cttgatatcg aattccgccc cccccccctc
     4321 tccctccccc gcccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4381 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4441 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4501 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4561 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4621 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4681 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4741 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4801 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4861 ggttttcctt tgaaaaacac gatgataata tggccacaac catgaccgag tacaagccca
     4921 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     4981 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5041 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5101 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5161 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5221 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5281 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5341 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5401 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5461 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     5521 agcgcccgac cgaaaggagc gcacgacccc atgcatcgat gatctagagc tcgctgatca
     5581 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5641 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5701 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5761 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5821 gcggaaagaa cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     5881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6001 cagtcgggaa acctgtcgtg ccagcggatc ctttgttact ttatagaaga aattttgagt
     6061 ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     6121 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     6181 tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca
     6241 caatatgatt atctttctag ggttaaagag ctccagcttt tgttcccttt agtgagggtt
     6301 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6361 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6421 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6481 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6541 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6601 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6661 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6721 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6901 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8461 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8521 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8581 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8641 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8701 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8761 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8821 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8881 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     8941 ccattcaggc tgcgcaa
//
