LOCUS       sequence-239570-        3245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglFusion compatible integration vector for Streptococcus 
            pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Keller LE, Rueff AS, Kurushima J, Veening JW
  TITLE     Three New Integration Vectors and Fluorescent Proteins for Use in 
            the Opportunistic Human Pathogen Streptococcus pneumoniae.
  JOURNAL   Genes (Basel). 2019 May 22;10(5). pii: genes10050394. doi: 
            10.3390/genes10050394.
  PUBMED    31121970
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2019-05-22"; volume: "10(5). pii: genes10050394. 
            doi"; pages: " 10.3390/genes10050394"
COMMENT     Sequence Label: pPEPX
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      203..274
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      290..317
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      1018..1606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1507..1526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcatgcggca aagcactcaa aagtttacct tatgggtgct tttttcgtgc ttttttgaaa
       61 agacaaaaaa aagaaccttg ccaagcaaga ttcttttgat agggtggtct ccgatcgtgt
      121 actgcagaaa actagtcgca aaaaaccccg cttcggcggg gttttttcgc tctagacggt
      181 gatcaacacg ctagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      241 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      301 gggtgggcct ttctgcgttt atagaattca tgagatctaa ataaataagg aggaaaatta
      361 atgccggatc cctccagtaa ctcgagaaaa aaaaaccgcg cccctgacag ggcgcggttt
      421 ttttttaagt ggacacctac gtatggtaac tgtacaggtc gctgaaactt ctatcgtcaa
      481 gaagaatcac caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa
      541 gatttctatg actgatattg cccatcagct ttccatctca acttcaactg ttattcgtaa
      601 gctcaatgat tttcacttta aacatgattt ttcgcgtctt cctgagatta tgtcttggga
      661 tgagtatgct tttacaaagg gaaagatgag cttcattgcg caagatttta acaatctcaa
      721 tatcatcact gttcttgagg gaagaacaca agctatcatt cgaaatcact ttcttcgcta
      781 cgatagagtt gtccgatgtc gcgtcaaaat tattactatg gatatgttta gcccttacta
      841 tgacttggct aaacagcttc gctttcaaat ttctaggctc aggctgaaac agtctcccag
      901 actgtttcac tcccgaatgc taaaatcgtt cttgatcgct ttcacattgg acgtcctgac
      961 catggagtca agtgtaaggc actggtacca ccccgtagaa aagatcaaag gatcttcttg
     1021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1141 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     1201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1681 atcccgcggc cgcagttaaa gaacttggac tgcgctctaa aaccaacgtg gtcagtctgg
     1741 acaagattat tattgataaa aaatcctcac tgataaccgg tttcccagag tttcggatgg
     1801 tttggaaggt ggtccgccag cgtgtggtgg atgatctggt atccgttctg gatacggact
     1861 atctggatat ggaactaatc ccaaatctca ctcgccaaat cgctgagcag tctatctatt
     1921 cttatataga aaatggcctc aaactcctta ttgattatgc tcagaaggaa atcaccattg
     1981 accactcaag cgaccgagac aagattctca tggacattgg caaagaccct tatgtcgttt
     2041 cgattaaatc aaaagtctat ctccaagacg gacgccaatt tcagtttacc gaaagtcgcc
     2101 ataagttaga aaaatttaga tttgtagatt ttgcaaaacg caagaaataa aagactgaga
     2161 caccagatca tggctagaaa gcttctagca aaaaactgga cggagtcccg tccagaaaag
     2221 taagcacttg ttattgcaat aaaattagcc taattgagag aagtttctat agaatttttc
     2281 atatacttaa cgagtgcttt cacctttgaa tatagtcctt cccacttatc atcacactct
     2341 ccccgatagc cttttctagc tatatccagt aaagttacat gctctttagg taaaagaggt
     2401 atagcccatt cagcagcgac atctttcgag gtaatttcac cagtagtcac tgtttgccac
     2461 attcgagcta gggttaaaat tacattacgc tcatcacctt ttatcccctc aattagttct
     2521 ggcaaagaat ccttaattgc tcttcgaata tctgtcaaag gtacggagac aagtatactt
     2581 gaagaatcag gaccaaatag agaaatacta ttctttcttg cttgtgctaa aacaatagcc
     2641 aaatcaggat catagcttgg ttcctgaatt tgtccattct caaattcacc cctgagccac
     2701 tcaccgtata taaattctct ttttggagga tattgccaag ggacaacttc actcctattt
     2761 ataaccgtaa cttcaagtgg tctaacagaa tccgtatttc caatccttcc tgatatagtc
     2821 attagtcttt ctgttagttt ttttcgagtt aattgaggta aactatgatt cacgacgact
     2881 agaacatcta catcgctgtt aatgcgtaaa ccaccattta ctgctgaacc aaatagatat
     2941 actccaacta ttgaacttcc aaataaatct tttacgattt ttaatgtttg aatcgcttga
     3001 tttggtattt ttccgttaat caaattgctc atgatttcac ctcgttgatt atgttcatat
     3061 aaagtttata ttgatactca atttacttac cctagattgg acatatactt aaattactgt
     3121 tcaataaagc tgaccgttag cgtttaagta catcctttca caatttgtct acagaacagt
     3181 actggcctac ctagtatagc attttgtgaa gtttttttct agtccaagct cacaaaaatc
     3241 caaag
//
