LOCUS       sequence-239571-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating SPARK NanoLuc-o-beta-2AR transmembrane component 
            lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Kim CK, Cho KF, Kim MW, Ting AY
  TITLE     Luciferase-LOV BRET enables versatile and specific transcriptional 
            readout of cellular protein-protein interactions.
  JOURNAL   eLife. 2019 Apr 3;8. pii: 43826. doi: 10.7554/eLife.43826.
  PUBMED    30942168
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-04-3"; volume: "8. pii: 43826. doi"; pages: " 
            10.7554/eLife.43826"
COMMENT     Sequence Label: pLX208-gLuc
            sp-NanoLuc-HA-o-beta-2AR-TS-eLOV-TEVcs-...
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1847..1964
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2081..2384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2385..2588
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2539..2559
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2611..2620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2668..3177
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             3178..3204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3217..3255
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
     CDS             5002..5022
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             5035..5058
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5059..5496
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5497..5841
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     misc_feature    5857..6445
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5910..5930)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6328..6339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6517..6750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6828..6949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6865..6884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6919..6938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6989..7124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7144..7163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7145..7163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7173..7190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7173..7189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7182..7204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7331..7786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7418..7437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7628..7649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7812..7916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7917..7985,7986..8777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8135..8154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8948..9536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9437..9456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9690..9707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9824..9845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9860..9865,9866..9883,9884..9890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9898..9914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9903..9925
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9922..9938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9922..9938
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9957..9977
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9959..9977
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagagcg ctgccgtctg gaccgatggc tgtgtggaag
     1741 tgctcgccga tagtggaaac agacgcccca gcactcgtcc tagggcaaag gatctgcagt
     1801 aatgagaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     1861 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1921 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     1981 acagcagaga tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa
     2041 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2101 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2161 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2221 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2281 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2341 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2401 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2461 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2521 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2581 gcagagctct ctggctaact gtcgggatcc gccaccatgg gagtcaaagt tctgtttgcc
     2641 ctgatctgca tcgctgtggc cgaggccgtc ttcacactcg aagatttcgt tggggactgg
     2701 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
     2761 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
     2821 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
     2881 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
     2941 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
     3001 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
     3061 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
     3121 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtat
     3181 ccgtacgacg taccagacta cgcaaacgga acagagggcc caaactttta cgttcccttc
     3241 tccaataaga ctggggtcgt gagaagccca tttgaggcgc ctcaatacta ccttgctgag
     3301 ccgtggcagt tttctatgct cgctgcttac atgttcttgc tgatcatgct ggggttccct
     3361 atcaatttcc tgacgctgta cgttatagca aagttcgaac gcctccaaac cgtgttgaac
     3421 tacatactcc ttaacctcgc ggttgccgac ctcttcatgg ttttcggggg tttcaccacc
     3481 accctctaca cctcccttca cggctacttc gtgttcggcc ctaccggatg caatctggaa
     3541 ggctttttcg caacgctggg gggggagatt gccctttgga gcctggtggt cttggccata
     3601 gagaggtacg tggtggtcac atccccattc aagtaccaga gtttgcttac aaagaacaag
     3661 gctatcatgg gggtcgcctt cacatgggtg atggcgctgg cttgcgctgc cccaccgctg
     3721 gtaggctggt cccggtatat tccggaggga atgcagtgca gttgtgggat cgactactac
     3781 accccacacg aagagactaa caacgagtct tttgtgattt atatgttcgt ggtccacttc
     3841 atcatccccc tgatagtgat ctttttctgt tacggcaggg tgttccaggt cgccaaaagg
     3901 cagctccaga agatcgacaa aagcgaaggc cgctttcaca gccccaatct tggacaggtt
     3961 gaacaggacg gcaggtcagg gcacgggctg cgacgcagtt ctaagttctg cctgaaggaa
     4021 cataaggcct tgagaatggt gatcatcatg gtaatcgcct tcctgatatg ctggcttcca
     4081 tacgctggcg tggcttttta tatattcacg caccaggggt cagattttgg gcctatcttt
     4141 atgaccatac ctgctttctt cgctaagacg agtgcggtgt ataacccagt gatatacatc
     4201 atgatgaaca aacaattcag aattgccttc caggaattgc tctgtctcag acgcagctct
     4261 tccaaagcgt acggaaatgg ctattcatct aacagcaacg gaaagactga ttatatgggc
     4321 gaagccagtg gctgccagct gggccaggaa aaagagagcg agcggctttg tgaagatccc
     4381 ccaggcactg agagcttcgt gaattgtcag ggaacagttc cgagtctctc tcttgattca
     4441 cagggacgca attgctctac caacgacagc cccctggaga cttcccaggt cgctccggcc
     4501 aagagcagga tcaccagcga gggcgagtac atccccctgg accagatcga catcaacgtg
     4561 ggtggagcta gcggcagccg ggcgaccacc ctggaacgta ttgaaaagag ctttgtgatt
     4621 accgatccgc gtctgccgga taacccgatt atttttgtga gcgatagctt tctgcagctg
     4681 accgaatata gccgtgaaga aattctgggc cgtaactgcc gttttctgca gggcccggaa
     4741 accgatcgtg cgaccgtgcg taaaattcgt gatgcgattg ataaccagac cgaagtgacc
     4801 gtgcagctga ttaactatac caaaagcggc aaaaaatttt ggaacctgtt tcatctgcag
     4861 ccgatgcgtg atcagaaagg cgatgtgcag tattttattg gcgtgcagct ggatggtacc
     4921 gaacgtgtgc gtgatgcggc ggaacgtgaa gctgtgatgc tggtcaagaa aaccgcggag
     4981 gaaatcgacg aagccgctaa ggaaaatctc tacttccaga tgggcggagg atccgactac
     5041 aaggatgacg acgataagaa gctactgtct tctatcgaac aagcatgcga tatttgccga
     5101 cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac
     5161 tgggagtgtc gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca
     5221 gaagtggaat caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa
     5281 gaccttgaca tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga
     5341 ttatttgtac aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag
     5401 actgatatgc ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag
     5461 agtagtaaca aaggtcaaag acagttgact gtatcggcca attttaatca aagtgggaat
     5521 attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc gaacctcata
     5581 acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat
     5641 aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca
     5701 ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg
     5761 tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca
     5821 ccaaacccaa aaaaagagta gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6241 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6421 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     6481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6541 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6721 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     6781 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     6841 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6901 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     6961 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7021 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7081 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     7141 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7201 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7261 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7321 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7801 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7861 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7921 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7981 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8041 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8101 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8161 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8221 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8281 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8341 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8401 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8461 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8521 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8581 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8641 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8701 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8761 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8821 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8881 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8941 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9001 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9061 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9121 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9181 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9241 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9301 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9361 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9421 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9481 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9541 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9721 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9781 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9841 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9901 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     9961 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
