LOCUS       sequence-239573-        7369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CaHygB plasmids that facilitate genetic manipulations in the fungal 
            pathogen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Basso LR Jr, Bartiss A, Mao Y, Gast CE, Coelho PS, Snyder M, Wong B
  TITLE     Transformation of Candida albicans with a synthetic hygromycin B 
            resistance gene.
  JOURNAL   Yeast. 2010 Dec;27(12):1039-48. doi: 10.1002/yea.1813. Epub 2010 Aug
            24.
  PUBMED    20737428
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1813"; 
            journalName: "Yeast"; date: "2010-12"; volume: "27"; issue: "12"; 
            pages: "1039-48"
COMMENT     Sequence Label: pYM70
FEATURES             Location/Qualifiers
     source          1..7369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1572..1594
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1586..1603
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1587..1603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3075..3091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3075..3091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3088..3110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3099..3115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3123..3129,3130..3147,3148..3153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3168..3189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3306..3323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3477..4065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3557..3576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4236..5027,5028..5096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4859..4878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5097..5201)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5880..6905
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     7342..7360
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgga tcataccaaa aaaaaatccg gggtagcgat gaggtagtgc aagttatacc
      241 agagagcctc aatttgaacg tggtactgca cacaaaccgt agccctaacc ctaattaaat
      301 gcacgtgacc cacacaattt tcaaccacca acaacacacc aaaaatgtat gtacactcgg
      361 agtggaaaat atttccccag gcataattgt ggttgccaat tatagtggag tgtttgttat
      421 tagtatagga gtactccttt tatgtggaaa aatcgaaaaa cataaaacag tgcagctata
      481 cactaacaaa tggtataccc atggtcagac aacaccaaac aacacacccg taatggttgg
      541 tttggctaaa tagagcatct cattttgtgt attattttat gttttggata tttttagttt
      601 gaaatttata taaaaatatt ttacttcaat tttcttgcca aattttgtac aaaaagtaaa
      661 aaatagaact tccaattttt gttagacaaa cttcaatcta acaaccggga tagtgttttg
      721 ggggctgtgt aacaccactg gtgtaataaa agtgccaata tttggaaatt aattttttgg
      781 tggtggacgt ttcttcctaa aacacctaga tgtgctagta taattgcaca accagcacgc
      841 acactgcgtc tggccggccc tggactacat tttgtctcta acattcgtag ctgcagcact
      901 gctacaaccc agtcaactgg ccacccggac atacatattc ccgttttgtg ccacagccac
      961 ccaactttcc ggctcaaaaa atggctccac acaatttggg caccccgatt acccctcccc
     1021 tgcagcgaca gcacatacac tttggtggtt gtggggctag agccctaaaa gtgaatttgg
     1081 tcacgtgaga gacagcagat aaggttcgca tttttttatt ttttcggttg cggccatatc
     1141 tagcagaaag caccgttccc cgttcgatca accgtagtta agctgctaag agcaataccg
     1201 agtagtgtag tgggagacca tacgcgaaac tattgtgctg caatctattt tttttagtaa
     1261 ggtatatttt ttttttgcat gaaccaaggg taatcgaccc tgctctatcc agtgatggtg
     1321 ataaagttgg gtggtgtcta acaaaaaggg ggagaaccag agggtgtata aataaggaga
     1381 agctgggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     1441 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1501 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     1561 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct
     1621 gcaggtcgac ttggaaatca ataacgtgtg ttggagacag aaccaagggg attggatggg
     1681 tgttgttagt gggaaaggtt tccaagcagt tctgatatga tttagagttt gtatgtacaa
     1741 tatagcacca tcaacatatg tgtaagatta actaactggt aagaatatat atatatatat
     1801 atacataaac aaagaagcac ataactcctc ttcttttttt tatttacaga gctgggactc
     1861 ttcgctcctt actacatgtt gtatgattat tctcttattt atatatattt taaaaaaata
     1921 atataatcac aactatttaa acaaaggtat ttaattagtt tacccgggga tccactagtc
     1981 cgcggttaat taaggtataa ctctttctta taaaaatcgg tttgtatgtg tgtatatgtg
     2041 ggagaaaaaa aaattacaaa gaaatcaaaa aggatctggg aaactttcaa actctatttt
     2101 atatcaaagt ttaattttat atgattgtaa taacaagtgg gcaaattctt tcaaaactat
     2161 tgaatgaaga aagaaaacca atgagctaaa acattctttc caaaaaaaac gaaaaacgaa
     2221 aactaaacac caaccccgga aaccgaacta tcatgaatcg tgtcagatgt ctaaacattg
     2281 tgtgaagatg agagaggaat gaattctcca gacacaaaca aacaccaaaa attttaataa
     2341 tccttgttca gtttttcttt gcccgacaaa aaaaataaac ctaaaaaaaa ttttcatttc
     2401 ggaaaaatca taaatcgctt ttcaccttca aataaacata aattgtaacg ttacatttaa
     2461 ctttctcata attaattatg attttggtgt cgggaaaata caatcatctt tgtccatata
     2521 ttttttggaa ggttttgtaa actgcttaaa attttttccc ccccactttc cccgatggaa
     2581 tgaaaaaata aattaatccg aagcttcccc aaaatttctt tcggacactg gccacaaaac
     2641 aagctttttt tcttggcaat atttatgctc acacatagca caaaaccagt ttgacaaaaa
     2701 aaaaagaact aaaacaatgg agaaaaaagt gttcccactt gtattctgtt cgtggcaaag
     2761 tattcctcta tgttcgcttc taattcccat aattacttta tgtgtttgtg tgctggtcat
     2821 ccaattccag tttcattatt ttcaaaatga aatgtatcga ccggtttgca ttgttatttg
     2881 gttagttgac taatacctca cattaccaat ttttgaaact caatttctgt ctggtttttc
     2941 aagcatcatt tcttatattt agagctaagc ctaaaaatat cttctgatca tccatccgaa
     3001 cgtttttgtg catagtctgg agtaaacaaa caatctagag gatccccggg taccgagctc
     3061 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3121 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3181 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3361 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3481 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3901 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4141 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4201 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4261 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4321 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4381 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4441 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4501 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4561 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4621 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4681 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4741 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4801 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4861 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4921 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4981 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5041 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5101 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5161 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5221 ccacctgacg tcgtatagtg cttgctgttc gatattgcta gaggcaaaga aaaaggatta
     5281 cctcacgcct aaaatggaat ttgaaaattg aggagatgag ttgagctttt gtcttttaca
     5341 ctttccctat tcaattgtgc cgcaaaaact tcaatatcta acacagtgtg aaatatttgt
     5401 tatgtatacc gaaagccaaa taccatttaa actggtattt caagatttca taaatcaagt
     5461 ttctggttaa attaattgct gttaaacaga acaagagctt cacattgatg ttggaataac
     5521 tactatagaa ttataaaata cacaaaacca ggtcgtgtac gcaataactt ttcaatgtat
     5581 gaaaaaattt tttttttttt tttgcttctt ctcttcgctt cttttttcta gttatcaatt
     5641 tggcaatgaa ttgatttgat tgccctttgt ttagtttact atgaaaaaaa attttttttt
     5701 tgtctctgtc agattttctc tcattttttt ttttgcctca taatgaacaa ctcaaaaaag
     5761 aatataaatt tctcatcaaa gacctttcca attgaaaatt ttttctttcc ttcttttcct
     5821 tttttttttt ctttctttct tcttttctaa gtatacattt tatataatca atcctcgaga
     5881 tgaaaaaacc agaattgact gctacttctg ttgaaaaatt tttgattgaa aaatttgatt
     5941 ctgtttctga tttgatgcaa ttgtctgaag gtgaagaatc aagagctttt tcttttgatg
     6001 ttggtggtag aggttatgtt ttgagagtta attcttgtgc tgatggtttt tataaagata
     6061 gatatgttta tagacatttt gcttctgctg ctttgccaat tccagaagtt ttggatattg
     6121 gtgaattttc tgaatctttg acttattgta tttcaagaag agctcaaggt gttactttgc
     6181 aagatttgcc agaaactgaa ttgccagctg ttttgcaacc agttgctgaa gctatggatg
     6241 ctattgctgc tgctgatttg tctcaaactt ctggttttgg tccatttggt ccacaaggta
     6301 ttggtcaata tactacttgg agagatttta tttgtgctat tgctgatcca catgtttatc
     6361 attggcaaac tgttatggat gatactgttt ctgcttctgt tgctcaagca ttggatgaat
     6421 tgatgttgtg ggctgaagat tgtccagaag ttagacattt ggttcatgct gattttggtt
     6481 ctaataatgt tttgactgat aatggtagaa ttactgctgt tattgattgg tctgaagcta
     6541 tgtttggtga ttctcaatat gaagttgcta atattttttt ttggagacca tggttggcat
     6601 gtatggaaca acaaactaga tattttgaaa gaagacatcc agaattggct ggttctccaa
     6661 gattgagagc ttatatgttg agaattggtt tggatcaatt gtatcaatct ttggttgatg
     6721 gtaattttga tgatgctgct tgggctcaag gtagatgtga tgctattgtt agatcaggtg
     6781 ctggtactgt tggtagaact caaattgcta gaagatcagc tgctgtttgg actgatggtt
     6841 gtgttgaagt tttggctgat tctggtaata gaagaccatc tactagacca agagctaaag
     6901 aataaggatc cgagtgaaat tctggaaatc tggaaatctg gttttgtatt cttgttattc
     6961 ttctttttgt tattacatat ataacttgtt acttttttaa aaaaatcttt gtttatttta
     7021 taaatatata aaactaaatt taagaaaaag agaaaaatgt tttatttgag agattgaaat
     7081 tttacttgaa tttagcttag cttttataaa gtattattat gtaaaaaaac aaaacaaata
     7141 tacattaaaa agttaagact ataaaatagc cacccaaggc atttctatat cttgttgttg
     7201 ttgttttcat cttctgtatc agaggaactt attttattat tttcgtcacg ggtattttct
     7261 cttgtttgat gattcatccc attcattcca tcataaaatg acgtctaaga aaccattatt
     7321 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
