LOCUS       sequence-239610-        9038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of IRF4 under a Doxycycline 
            inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Patil A, Manzano M, Gottwein E
  TITLE     CK1alpha and IRF4 are essential and independent effectors of 
            immunomodulatory drugs in primary effusion lymphoma.
  JOURNAL   Blood. 2018 Aug 9;132(6):577-586. doi: 10.1182/blood-2018-01-828418.
            Epub 2018 Jun 28.
  PUBMED    29954751
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1182/blood-2018-01-828418"; journalName: "Blood"; date: 
            "2018-08-9- 9"; volume: "132"; issue: "6"; pages: "577-586"
COMMENT     Sequence Label: pCW-IRF4-Puro
FEATURES             Location/Qualifiers
     source          1..9038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1929..2046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2100..2414
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2179..2197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2215..2233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2251..2269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2286..2304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2322..2340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2387..2411
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3845..4355
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4274..4292
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4376..4972
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4376..4395)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4872..4892
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4982..5038
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5039..5656,5657..5662,5663..5698,5699..5701,5702..5737,
                     5738..5740,5741..5776,5777..5785)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5825..5845)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6243..6254)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6459..6692
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6764..6885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6801..6820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6855..6874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6925..7060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7060..7131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7132..7200,7201..7992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7350..7369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8163..8751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8652..8671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8905..8922
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggacaa ttctcgacct cgagacaaat ggcagtattc
     1921 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1981 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2041 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcgaat cgatatgtcg
     2101 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     2161 gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     2221 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2281 gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     2341 acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
     2401 accgtcagat cgcctggaga attgggccgc caccatgaac ctggagggcg gcggccgagg
     2461 cggagagttc ggcatgagcg cggtgagctg cggcaacggg aagctccgcc agtggctgat
     2521 cgaccagatc gacagcggca agtaccccgg gctggtgtgg gagaacgagg agaagagcat
     2581 cttccgcatc ccctggaagc acgcgggtaa acaagattat aatcgtgaag aggacgccgc
     2641 gctcttcaag gcttgggcac tgtttaaagg aaagttccga gaaggcatcg acaagccgga
     2701 ccctcccacc tggaagacgc gcctgcggtg cgctttgaac aagagcaatg actttgagga
     2761 actggttgag cggagccagc tggacatctc agacccgtac aaagtgtaca ggattgttcc
     2821 tgagggagcc aaaaaaggag ccaagcagct caccctggag gacccgcaga tgtccatgag
     2881 ccacccctac accatgacaa cgccttaccc ttcgctccca gcccagcagg ttcacaacta
     2941 catgatgcca cccctcgacc gaagctggag ggactacgtc ccggatcagc cacacccgga
     3001 aatcccgtac caatgtccca tgacgtttgg accccgcggc caccactggc aaggcccagc
     3061 ttgtgaaaat ggttgccagg tgacaggaac cttttatgct tgtgccccac ctgagtccca
     3121 ggctcccgga gtccccacag agccaagcat aaggtctgcc gaagccttgg cgttctcaga
     3181 ctgccggctg cacatctgcc tgtactaccg ggaaatcctc gtgaaggagc tgaccacgtc
     3241 cagccccgag ggctgccgga tctcccatgg acatacgtat gacgccagca acctggacca
     3301 ggtcctgttc ccctacccag aggacaatgg ccagaggaaa aacattgaga agctgctgag
     3361 ccacctggag aggggcgtgg tcctctggat ggcccccgac gggctctatg cgaaaagact
     3421 gtgccagagc aggatctact gggacgggcc cctggcgctg tgcaacgacc ggcccaacaa
     3481 actggagaga gaccagacct gcaagctctt tgacacacag cagttcttgt cagagctgca
     3541 agcgtttgct caccacggcc gctccctgcc aagattccag gtgactctat gctttggaga
     3601 ggagtttcca gaccctcaga ggcaaagaaa gctcatcaca gctcacgtag aacctctgct
     3661 agccagacaa ctatattatt ttgctcaaca aaacagtgga catttcctga ggggctacga
     3721 tttaccagaa cacatcagca atccagaaga ttaccacaga tctatccgcc attcctctat
     3781 tcaagaatga ctagcgacac cggtgcagtc gacaccggaa ttcaacctta attaacgacg
     3841 cgtcggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     3901 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     3961 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     4021 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     4081 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     4141 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     4201 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     4261 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     4321 ccctcgttga ccgaatcacc gacctctctc cccagcaatt ccctgcaggg ccaccatgac
     4381 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4441 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4501 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4561 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     4621 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4681 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4741 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4801 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4861 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4921 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccggatccgg
     4981 cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat
     5041 gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
     5101 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     5161 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     5221 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     5281 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     5341 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     5401 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     5461 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     5521 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     5581 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     5641 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
     5701 agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt
     5761 tgaccttgac atgctccccg ggtaactaag taaggatcga tccaagatat cgtattctta
     5821 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5881 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5941 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6001 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6061 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6121 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6181 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6241 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6301 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6361 ctgtttcgcc tcggcgtccg gactagaggt acctttaaga ccaatgactt acaaggcagc
     6421 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6481 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6541 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6601 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6661 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     6721 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     6781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6841 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     6901 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6961 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7021 gagctattcc agaagtagtg aggaggcttt tttggaggcc tttcaaatat gtatccgctc
     7081 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7141 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7261 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7321 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7381 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7501 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7681 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7741 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7981 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8821 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8881 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8941 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     9001 tttcccgact ggaaagcggg cagtgagcgc aacgcaat
//
