LOCUS       sequence-239613-        1386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses red fluorescent calcium sensor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Kerruth S, Coates C, Durst CD, Oertner TG, Torok K
  TITLE     The kinetic mechanisms of fast-decay red-fluorescent genetically 
            encoded calcium indicators.
  JOURNAL   J Biol Chem. 2019 Mar 15;294(11):3934-3946. doi: 
            10.1074/jbc.RA118.004543. Epub 2019 Jan 16.
  PUBMED    30651353
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004543"; journalName: "J Biol Chem"; date: 
            "2019-03-15- 15"; volume: "294"; issue: "11"; pages: "3934-3946"
COMMENT     Sequence Label: pGP-CMV-NES-fRCaMP2
FEATURES             Location/Qualifiers
     source          1..1386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..120
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(124..132,133..147)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             175..231
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 atgctgcaga acgagcttgc tcttaagttg gctggacttg atattaacaa gactggagga
       61 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      121 cgggatctgt acgacgatga cgataaggat ctcgcaacaa tggtcgactc atcacgtcgt
      181 aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc agcgatcaac
      241 tgcgaaatga tgtacccagc ggatggtggt ctgcgtggtt acactcacat ggcgctgaaa
      301 gttgatggcg gcggtcacct gtcctgttct ttcgtgacca cctaccgctc caaaaagact
      361 gtcggcaaca ttaagatgcc tgccattcat agcgtcagcc accgtctgga gcgcctggag
      421 gagagcgata acgaaatgtt tgtcgtacag cgtgaacacg cagttgccaa gtttgtgggc
      481 ctgggtggtg gcggcggtac cggagggagc atgaactccc tgatcaagga gaacatgcgt
      541 atgaaagtgg ttctggaagg ctccgtaaac ggccaccagt tcaaatgcac tggtgaaggc
      601 gaaggcaacc cgtatatggg cacccagact atgcgtatca aagtgatcga gggtggtccg
      661 ctgccgtttg cgttcgacat cctggcgacg tcctttatgt atggctcccg taccttcatc
      721 aaatatccga aaggcatccc ggatttcttt aagcagtcct tcccggaagg ttttacctgg
      781 gaacgtgtga cccgttacga agacggcggc gtaattaccg ttatgcaaga cacgtctctg
      841 gaggatggct gcctggtgta tcacgtgcag gttcgcggtg tgaacttccc gagcaatggt
      901 gctgtaatgc aaaagaaaac caaaggttgg gagcctacgg actcccaaac cactgaagag
      961 cagatcgcag aatttaaaga ggctttctcc ctatttgaca aggacgggga tgggacaata
     1021 acaaccaagg agctggggac ggtgatgcgg tctctggggc agaaccccac agaagcagag
     1081 ctgcaggaca tgatcaatga agtagatgcc gacggtgacg gcacaatcga cttccctgag
     1141 ttcctgatta tgatggcaag aaaaatgaaa tacacagaca gtgaagaaga aattagagaa
     1201 gcgttcggcg tgtttgctaa ggatggcaat ggctacatca gtgcagcaga gcttcgccac
     1261 gtgatgacaa accttggaga gaagttaaca gatgaagagg ttgatgaaat gatcagggaa
     1321 gcagacagcg atggggatgg tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg
     1381 aagtag
//
