LOCUS       sequence-23962-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Takanaga H, Frommer WB
  TITLE     Facilitative plasma membrane transporters function during ER 
            transit.
  JOURNAL   FASEB J. 2010 Aug . 24(8):2849-58.
  PUBMED    20354141
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J. 
            2010 Aug . 24(8):2849-58."
COMMENT     Sequence Label: pcDNA3.2/v5-DEST hGlut1
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagcagagct ctctggctaa ctagagaacc cactgcctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa
      121 agcaggctcc gcggccgccc ccttcaccat ggagcccagc agcaagaagc tgacgggtcg
      181 cctcatgctg gccgtgggag gagcagtgct tggctccctg cagtttggct acaacactgg
      241 agtcatcaat gccccccaga aggtgatcga ggagttctac aaccagacat gggtccaccg
      301 ctatggggag agcatcctgc ccaccacgct caccacgctc tggtccctct cagtggccat
      361 cttttctgtt gggggcatga ttggctcctt ctctgtgggc cttttcgtta accgctttgg
      421 ccggcggaat tcaatgctga tgatgaacct gctggccttc gtgtccgccg tgctcatggg
      481 cttctcgaaa ctgggcaagt cctttgagat gctgatcctg ggccgcttca tcatcggtgt
      541 gtactgcggc ctgaccacag gcttcgtgcc catgtatgtg ggtgaagtgt cacccacagc
      601 ccttcgtggg gccctgggca ccctgcacca gctgggcatc gtcgtcggca tcctcatcgc
      661 ccaggtgttc ggcctggact ccatcatggg caacaaggac ctgtggcccc tgctgctgag
      721 catcatcttc atcccggccc tgctgcagtg catcgtgctg cccttctgcc ccga
//
