LOCUS       sequence-239620-        1413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses red fluorescent calcium sensor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Kerruth S, Coates C, Durst CD, Oertner TG, Torok K
  TITLE     The kinetic mechanisms of fast-decay red-fluorescent genetically 
            encoded calcium indicators.
  JOURNAL   J Biol Chem. 2019 Mar 15;294(11):3934-3946. doi: 
            10.1074/jbc.RA118.004543. Epub 2019 Jan 16.
  PUBMED    30651353
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.004543"; journalName: "J Biol Chem"; date: 
            "2019-03-15- 15"; volume: "294"; issue: "11"; pages: "3934-3946"
COMMENT     Sequence Label: pGP-CMV-NES-fRGECO1
FEATURES             Location/Qualifiers
     source          1..1413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..120
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(124..132,133..147)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             175..231
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     927..946
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgctgcaga acgagcttgc tcttaagttg gctggacttg atattaacaa gactggagga
       61 ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      121 cgggatctgt acgacgatga cgataaggat ctcgcaacaa tggtcgactc atcacgtcgt
      181 aagtggaata aggcaggtca cgcagtcaga gctataggtc ggctgagctc acccgtggtt
      241 tccgagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg
      301 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      361 gtgcagctgc ccggcgccta catcgtggac atcaagttgg acatcgtgtc ccacaacgag
      421 gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg
      481 gacgagctgt acaagggagg tacaggcggg agtctggtga gcaagggcga ggaggataac
      541 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      601 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      661 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      721 ttcatgtacg gctccaaggc ctacattaag cacccagccg acatccccga ctacttcaag
      781 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      841 attcacgtga accaggactc ctccctgcag gacggcgtat tcatctacaa ggtgaagctg
      901 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      961 gctacgcgtg acgacctgac tgaagagcag atcgcagaat ttaaagaggc tttctcccta
     1021 tttgccaagg acggggatgg gacgataaca accaaggagc ttgggacggt gttccgatcg
     1081 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     1141 ggtgacggca cattcgactt ccctgagttc ctgacgatga tggcaagaaa aatgaatgac
     1201 acagacagtg aagaggaaat tcgcgaagcg ttccgcgtgt ttgataagga cggcaatggc
     1261 tacatcggcg cagcagagct tcgccacgtg atgacagacc ttggagagaa gttaacagat
     1321 gaggaggttg atgaaatgat cagggtagca gacatcgatg gggatggtca ggtaaactac
     1381 gaagagtttg tacaaatgat gacagcgaag tag
//
