LOCUS       sequence-239622-       11800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 
            isolate; C-terminal truncation; QES mutant with enhanced binding to 
            PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-191084-QES.i06.c07-754*
FEATURES             Location/Qualifiers
     source          1..11800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(710..728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        796..900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(901..969,970..1761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1119..1138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1932..2520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2421..2440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2951..3096
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3139..4176
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4174..4193)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4218..4266
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4920..5299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5300..5503
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5454..5474
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5500..5524
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7806..7940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7830..7849)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7884..7903
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8083..8105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8665..10454
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cctgctcctg ttccaccgtg ggtccctttg cagccaatgc aacttggacg tttttggggt
       61 ctccggacac catctctatg tcttggccct gatcctgagc cgcccggggc tcctggtctt
      121 ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat ggttatcacc ccctcttctt
      181 tgaggtccac tgccgccgga gccttctggt ccagatgtgt ctcccttctc tcctaggcca
      241 tttccaggtc ctgtacctgg cccctcgtca gacatgattc acactaaaag agatcaatag
      301 acatctttat tagacgacgc tcagtgaata cagggagtgc agactcctgc cccctccaac
      361 agccccccca ccctcatccc cttcatggtc gctgtcagac agatccaggt ctgaaaattc
      421 cccatcctcc gaaccatcct cgtcctcatc accaattact cgcagcccgg aaaactcccg
      481 ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag gcctcaaatt cctcgtcccc
      541 ctttttgctg gacggtaggg atggggattc tcgggacccc tcctcttcct cttcaaggtc
      601 accagacaga gatgctactg gggcaacgga agaaaagctg ggtgcggcct gtgaggatca
      661 gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata
      721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1141 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1441 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2041 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg aagctgtccc
     2581 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     2641 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     2701 aagacgtagc ccagcgcgtc ggccccgaga tgcgccgcgt gcggctgctg gagatggcgg
     2761 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     2821 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct gcttcatccc
     2881 cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt tgcatgtctt
     2941 tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     3001 gatgcagtcg gggcggcgcg gtccgaggtc cacttcgcat attaaggtga cgcgtgtggc
     3061 ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc gtcaacagcg tgccgcagat
     3121 cccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     3181 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     3241 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     3301 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     3361 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
     3421 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     3481 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     3541 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     3601 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     3661 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     3721 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     3781 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     3841 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     3901 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     3961 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     4021 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     4081 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     4141 actcgtccgg atcgggagat gggggaggct aactgaaaca cggaaggaga caataccgga
     4201 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
     4261 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4321 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4381 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gccactggcc
     4441 ccgtgggtta gggacggggt cccccatggg gaatggttta tggttcgtgg gggttattat
     4501 tttgggcgtt gcgtggggtc aggtccacga ctggactgag cagacagacc catggttttt
     4561 ggatggcctg ggcatggacc gcatgtactg gcgcgacacg aacaccgggc gtctgtggct
     4621 gccaaacacc cccgaccccc aaaaaccacc gcgcggattt ctggcgtgcc aagctagtcg
     4681 accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct
     4741 gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc
     4801 catattagtc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     4861 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     4921 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4981 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5041 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5101 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5161 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5221 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
     5281 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
     5341 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     5401 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
     5461 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     5521 gatctctaga agctgggtac cagctgctag cgccaccatg ccgatggggt ctctccagcc
     5581 gctggccacc ctctacctgt tgggtatgtt ggtggcgtcc gtgctggcga cagagaatct
     5641 gtgggttaca gtctactacg gagttccagt ctggagggac gcagagacaa cacttttttg
     5701 tgccagcgac gctaaagcgt atgacactga aatgcataat gtctgggcaa ctcatgcctg
     5761 tgttcccaca gacccgaatc cacaagaaat agacttggag aatgtaacgg agaaatttaa
     5821 catgtggaaa aataacatgg ttgaacaaat gcatacggat ataatcagcc tttgggacca
     5881 atccctgaag ccgtgcgtta agttgacaga cttgtgtgtt actctgaact gcacagccat
     5941 taccaatgat acgaggggga atgagactgg tatcaatcgg accgtcgaga cgaccgaaat
     6001 gaccaactgt tcattcaata tgaccactga acttagggac cgaaaaaaga aggtcaatgc
     6061 gcttttttat aagcttgaca tcgttcagat aggagaaaat tcatctagcc agtaccgcct
     6121 cattaattgt aataccagtg ttattacaca agcctgccca aaaatgacct tcgagccaat
     6181 tccaattcat tattgcgcgc ctgctggatt tgccatcctt aagtgcaagg acaaagagtt
     6241 caacgggaca ggaacttgta ggaacgtgtc cagtgtgcaa tgcactcacg gtataaaacc
     6301 cgtcatgagc acgcaacttt tgctcaacgg gtccctggcg gaaggacagg tgaagatccg
     6361 gtcagagaac atctcagaca atgcaaaaac catcatcgtc cagttgaatg agtctgttcc
     6421 aatcaattgt actagaccaa acaacaatac acgaaggggg atccacctgg gtccgggcca
     6481 aacattcttt gctaccgaca taattggtga cattcgccaa gctcactgta atgttagcga
     6541 atcaaaatgg aacaaggcgc tccaggaagt tgtgaaacag ctgaggcagc actggaacaa
     6601 aacgataatc ttcaaatcct catccggtgg ggatctcgaa atcacgactc actccttcaa
     6661 ctgtggaggt gagtggttct actgtaacac ctcaggcctg ttcaatagca catggaacat
     6721 agccggcaac agaacgaatg atacgaagtc aaatgaaaca atcaccctcc cgtgtcgaat
     6781 aaaacagatc gtaaatgttt ggcaaagggt aggacaagcg atctacgcgc ccccaattgc
     6841 aggagtcatt cggtgcaatt caaacatcac aggcctcctc ttggtaagag atgggggagc
     6901 tactaataat acagacgaaa cgttccgccc tggaggcggg aacatgcgcg ataattggag
     6961 aagtgagttg tataaatata aggtggtcaa gatagagcct ctgggcgttg caccgaccag
     7021 ggcgcgcagg cgcgtggtgg aaagggagaa gagggctgta ggactcggag ccgtatttat
     7081 tggatttctt ggggcagcgg gttctaccat gggtgcagct tccgtgacct tgaccgtaca
     7141 agccaggcaa tatctttctg gaatcgttca acaacaaagc aatcttctcc aggcaatcga
     7201 ggcccagcag catctcttga aacttacagt ctggggcata aagcagctcc aggcgcgcgt
     7261 gctcgcagtt gagagatatt tgaaagacca acagctgctt ggtatttggg gctgttctgg
     7321 aaaattgata tgcccgacta ccgtaccatg gaacagtagc tggtcaaaca aatcacaaaa
     7381 cgaaatctgg gataatatga cctggcttca atgggacaag gagatttcaa attacacaca
     7441 gcttatatat tcattgattg aggaaagcca aaatcagcag gagaagaacg agcaggagct
     7501 gttggcgctg gacaagtggg catctttgtg gaattggttc gacatttcca agtggctttg
     7561 gtatatcaga atatttataa tgatcgtggg cgggttgata ggacttagaa ttgtattcac
     7621 agttctcagc atcattaata gggttcgcca gggttactcc ccgctctctt ttcagacaca
     7681 tccgccatct ccaacaggtc ctgataggcc tgggcggatt gaagaagagg gtggtgagca
     7741 aggacgggat agaagcacca ggctggtatc tggattcttg tgataactcg aggccggcaa
     7801 ggccggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7861 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7921 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     7981 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     8041 atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     8101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     8161 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg
     8221 atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca
     8281 gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt
     8341 gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat
     8401 ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt
     8461 ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc
     8521 tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat
     8581 cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt
     8641 tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata
     8701 ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg
     8761 aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca
     8821 cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg
     8881 gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc
     8941 attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga
     9001 ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca
     9061 gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg caataaatac
     9121 tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca
     9181 agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc
     9241 tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga
     9301 accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg
     9361 actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc
     9421 cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca
     9481 gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga
     9541 ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc
     9601 cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct
     9661 ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata
     9721 ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca
     9781 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     9841 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     9901 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     9961 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata
    10021 gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata
    10081 tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata
    10141 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
    10201 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
    10261 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
    10321 tccgggtagc atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga
    10381 gtgggagtgc tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc
    10441 ttgtcctttt cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag
    10501 ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg
    10561 tgttgagcgc ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg
    10621 aggacgaaaa tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct
    10681 actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc
    10741 tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc
    10801 tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg
    10861 aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc
    10921 gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt
    10981 ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc
    11041 gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat
    11101 agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa
    11161 cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag
    11221 agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg
    11281 ctttccaaac caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt
    11341 gcttgggcct tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc
    11401 aggctcacca tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg
    11461 gtggaaaaat ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga
    11521 ctgactactg ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct
    11581 ttcacgactt ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc
    11641 tcgaccccgg cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc
    11701 tccacctcct gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctcc
    11761 tgctcctgcc cctcctgctc ctgcccctcc cgctcctgct
//
