LOCUS       sequence-239624-       10285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; 
            QES mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH115gp160-QES.c14
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(673..691)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        759..863
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(864..932,933..1724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1082..1101)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1895..2483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2384..2403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2914..3059
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3102..4139
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4137..4156)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4181..4229
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4883..5262
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5263..5466
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5417..5437
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5463..5487
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8141..8275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8165..8184)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8219..8238
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8418..8440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg
       61 agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc
      121 catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg
      181 tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga
      241 ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag
      301 tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca
      361 gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt
      421 actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc
      481 caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac
      541 ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag
      601 ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt
      661 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      721 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      781 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      841 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      901 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      961 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1021 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1081 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     1141 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1201 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1261 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1321 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1381 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     1441 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1501 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1561 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1621 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1681 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1801 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1861 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1921 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1981 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2041 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2101 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2161 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2221 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2281 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2341 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2401 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2461 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2521 tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     2581 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     2641 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg agatgcgccg
     2701 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     2761 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     2821 gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
     2881 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
     2941 tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag gtccacttcg
     3001 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaac
     3061 agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag cctgaactca
     3121 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     3181 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     3241 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     3301 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     3361 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     3421 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     3481 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     3541 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     3601 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     3661 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     3721 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     3781 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     3841 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     3901 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     3961 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     4021 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     4081 atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag gctaactgaa
     4141 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     4201 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4261 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4321 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4381 aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat ggggaatggt
     4441 ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact
     4501 gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac
     4561 acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga
     4621 tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc atcgcagatc
     4681 cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca
     4741 ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt
     4801 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
     4861 tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
     4921 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4981 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     5041 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     5101 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
     5161 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
     5221 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     5281 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5341 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
     5401 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5461 agagctcgtt tagtgaaccg tcagatctct agaagctggg taccagctgc tagcgccacc
     5521 atgccgatgg gctcccttca accgctcgct acgctgtatc ttctcggcat gttggtcgct
     5581 agtgtgctgg ctgtcggtaa tctttgggtt acagtgtact atggtgtacc agtgtggaga
     5641 gaggcaacca ctactctttt ctgtgcttcc gacgccaaag cttatgacgc ggaagttcac
     5701 aatgtttggg caacgcatgc ctgcgtacct acagacccca accccctcga aattgatctc
     5761 aaaaatgtta ctgagaactt taatatgtgg gaaaataaga tggtggatca aatgcatgaa
     5821 gatgtaatca gtctttggga ccagagcttg aagccatgcg tgaaactgac gcccttgtgt
     5881 gtgacattgg aatgctctaa tgtaaccttg gaacggtcaa atgtttcaag tagtaatgac
     5941 acccagaata gaacgcacca tgagtccatc aaagagatga aaaattgcag cttcaacgct
     6001 accaccgagc tccgagataa aaagcaaaag gtgcacgcac tcttttatcg cttggacatc
     6061 gtaccgttgg acgagaagaa aaatagcagc gaaaattctt ccgaaagtta caggctcatc
     6121 aattgtaata ccagtgcaat aacacaggcg tgtccaaaga tgactttcga tccgatccca
     6181 atccactact gcactccagc aggatatgcg atactcaagt gtaaggacaa gatgttcaat
     6241 ggaaccggac cttgccataa tgtgtcaaca gtccagtgta cgcacggaat taagccaacc
     6301 atgtctacgc agcttctctt gaacggtagc ctcgccgagg gggagataat aattcgatca
     6361 gagaacttga cagacaatgc aaagccaatc atagtacatc tgaatcaatc tgtagaaatc
     6421 aactgcacac ggccctccaa taacactaga cagagtatca gaatcgggcc ggggcaaacc
     6481 ttttacgcca ctggagagat catcggcgac attagacagg cgcactgtaa catcagcagg
     6541 gcgaggtgga atgaaaccct tcaaaaggtg ggtaaaaaac tcgctgagca ctttcagaat
     6601 aagacaatca agtttaactc atcttcagga ggagatctcg aaattacaac tcacagtttc
     6661 aattgcagag gcgagttttt ctattgcaac acttcaggtc tcttcaacgg cacatacatg
     6721 cccaacggca cttttatcgg ggccgaaggc aattcaacta taacaattcc ttgccgaata
     6781 aagcaaatca ttaatatgtg gcaagaagta ggcagagcca tgtacgcccc tccaatcgct
     6841 ggcaatataa cctgtaatag taatatcact ggcctgctcc tcgtcaggga cgggggtaat
     6901 atcattgggc aaaataatac tgagacgttc aggccgcttg ggggtaacat gaaagataat
     6961 tggagaagtg aactttataa atataaggta gtggaaataa agcccttggg catagctccc
     7021 accgacgcga agcggagggt cgtagaacga gagaagcgcg cggtgggttt gggtgccgta
     7081 tttctgggat tccttggagc cgctggatcc acaatggggg cggcgtctct cacgctcaca
     7141 gttcaagcga ggcaacttct gagtggaatc gtacagcaac agaacaacct gcttagagcg
     7201 atcgaagctc agcagcattt gcttcaattg actgtatggg ggatcaagca gctgcaggcc
     7261 agggtgcttg ccattgagcg gtacttgcaa gaccagcaat tgctggggat ctggggttgt
     7321 agcggaaaac ttatttgcac gaccgccgta ccgtggaacg attcatggag caacaagtcc
     7381 ttgacagaca tctggaacgg cacgacatgg atgcaatggg ataaggagat tagcacatac
     7441 accgacatca tatattccct gcttgaagac tcacagattc aacaggaaaa gaatgagaag
     7501 gacctgttgg ctctggactc atggaaaaat ttgtggaatt ggttctccat cacaaactgg
     7561 ctgtggtata ttaagatatt cataatgatt gtaggggggc ttatcggctt gcgcattatt
     7621 tttgctgtgc tctccatagt gaatcgggta aggcaaggat actccccgct ctccttccag
     7681 acacttatac ctaatagcgg cggtcctgac cggttgggtc gcattgaaga agaaggcggc
     7741 gaacaggaca aagacagatc catccgcctc gtatctggat tcctcgcatt ggcgtgggac
     7801 gatctccggt ccctctgtct gtttagttat caccgcctga gagatttcat tctcctgact
     7861 gcacgagtgg tggagctgct gggacggaat agccttcgag ggctccagcg aggttgggag
     7921 gcccttaagt atctgggctc cctggtacag tactgggggc aagaacttaa gaagagcgcc
     7981 atttcactgt tcgataccat agctattgcc gtggcagaag gtactgatcg gatcatagag
     8041 cttgtacaag gcttttgtag ggcgatctat aacattccga gacgcattcg ccagggattt
     8101 gaggcggcat tgcagtagta actcgaggcc ggcaaggccg gatccagaca tgataagata
     8161 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     8221 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     8281 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     8341 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     8401 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     8461 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     8521 cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc
     8581 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     8641 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     8701 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     8761 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     8821 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     8881 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     8941 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     9001 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     9061 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     9121 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     9181 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     9241 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     9301 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     9361 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     9421 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     9481 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     9541 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     9601 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     9661 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     9721 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     9781 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     9841 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     9901 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     9961 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
    10021 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
    10081 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
    10141 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
    10201 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
    10261 taatctatat ctgggtagca tatgc
//
