LOCUS       sequence-239625-       10065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160-754*
FEATURES             Location/Qualifiers
     source          1..10065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(734..752)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        820..924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(925..993,994..1785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1143..1162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1956..2544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2445..2464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2975..3120
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3163..4200
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4198..4217)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4242..4290
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4944..5323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5324..5527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5478..5498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5524..5548
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7161..7172
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    7878..8012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7902..7921)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7956..7975
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8155..8177)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc tttgcagcca
       61 atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg ccctgatcct
      121 gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt
      181 ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc tggtccagat
      241 gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc gtcagacatg
      301 attcacacta aaagagatca atagacatct ttattagacg acgctcagtg aatacaggga
      361 gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat ggtcgctgtc
      421 agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct catcaccaat
      481 tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct gagcctcaag
      541 ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg attctcggga
      601 cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa cggaagaaaa
      661 gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca tgagaattct
      721 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      781 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      841 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      901 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      961 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     1021 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     1081 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     1141 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     1201 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1261 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1321 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1381 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1441 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     1501 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1561 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1621 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1681 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1741 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1801 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1861 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1921 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1981 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2041 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2101 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2161 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2221 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2281 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2341 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2401 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2461 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2521 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2581 ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     2641 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     2701 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc
     2761 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     2821 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
     2881 cgaggtgccg ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc
     2941 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt
     3001 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc
     3061 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa
     3121 cagcgtcaac agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc
     3181 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     3241 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     3301 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     3361 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     3421 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     3481 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     3541 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     3601 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     3661 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     3721 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     3781 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     3841 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     3901 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     3961 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     4021 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     4081 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     4141 gatagtggaa accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga
     4201 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     4261 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     4321 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     4381 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     4441 caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg
     4501 tttatggttc gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac
     4561 tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga
     4621 cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg
     4681 atttctggcg tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat
     4741 ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac
     4801 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
     4861 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     4921 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     4981 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     5041 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     5101 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     5161 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
     5221 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
     5281 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     5341 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     5401 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     5461 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     5521 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagcgccac
     5581 catgccaatg ggtagtcttc agcctctcgc caccttgtat ctgctcggta tgctggttgc
     5641 cagcgttctg gctaacttgt gggtgaccgt atactacggg gtcccggtat ggaaggaggc
     5701 aaacactaca ctcttctgtg cttccgacgc aaaggggtat gatacggaga agcataatgt
     5761 ctgggcgaca catgcatgtg tgcctacaga cccaaacccg caggaagtcc tccttggtaa
     5821 taacgtgacc gagaacttta atatgtggaa aaataacatg gtagagcaga tgcatgaaga
     5881 cattataagc ttgtgggatc aatctcttaa gccgtgtgtg aagctgacac cgctttgcgt
     5941 aacactcaat tgtacggatt tgacgaaaaa tagcactacc aataactcca ccgctactaa
     6001 caataccacg aacaatagta ccgaaaaaat ggagatggac aaaggcgaga tgaagaattg
     6061 ctcctttaat atcacaacaa atattcgaga taagatgcag aaagaatacg ctttgctgta
     6121 caagcttgac atcgtgccga tcgacaatgg caagaataac agcaacacca attatcgcct
     6181 gatttcatgt aatacctcag taattacgca atcatgtccc aaagtgtcat ttcagcccat
     6241 tcctattcat tattgtgcac ccgccggttt tgcaatactt aagtgtaata ataagacctt
     6301 taatggaagc gggccttgta ctaatgtgtc ttccgtgcaa tgcactcacg gtatccgacc
     6361 tgttgtttca acccagcttt tgctgaacgg aagtctcgct gaagaagaaa tcgtgattag
     6421 gtccgctaac ttctctgaca acacgaagac tataattgtc caacttaagg aagcggtcga
     6481 aataaactgt acgcgcccga ataataacac gcgaaaatca attaacatcg ggcctgggcg
     6541 agctttttat gctactggca acataatagg ggacatccgc caagctcatt gtaacatatc
     6601 acggacaaag tggaataata cgttgacaga aattgctaag aagctgaaca agcagtacga
     6661 gaatagaaca atagcattta atcaatcctc cggaggcgat cttgagatcg tcatgcattc
     6721 attcaactgc ggtggagaat ttttctattg caacacttcc cagctgttca gagatacgtg
     6781 gaacaacact tggaacggca tatggaacaa cgatacggag gtcaacgaga ctatcacact
     6841 tccgtgtaga atcaaacaaa taatcaatat gtggcaagaa gtcgggaagg cgatgtatgc
     6901 cccgccaatc cgcggccaaa tcaattgctc atcaaatatt acgggactcc tgctcacccg
     6961 cgacggggga gaaagcaaca agaccataga ggtcttccgg cccggtggcg gagacatgag
     7021 ggacaactgg aggtccgagc tgtataagta caaagttgtt aagatagagc ccctcggggt
     7081 tgctcccacc aaagctaaaa gaagagtggt gcaacgagag aaaagagccg tcggcttggg
     7141 tgccctcttc gtcggatttt tgggagctgc aggtagcaca atgggtgctg cctctataac
     7201 gctgaccgta caagctcgac agcttcttag tggtatcgtt caacaacaaa acaatctctt
     7261 gcgagcaata gaagcccaac agcatttgct tcaactgaca gtatggggcg ttaagcagtt
     7321 gcaagcgcgg gtcttggcgg tcgaacgata cctgaaagac cagcaactcc tgggcttgtg
     7381 gggatgcagc ggcaaactta tatgtaccac agctgtacct tggaacacca gctggagtaa
     7441 caagagttac gattatatct gggacaacat gacgtggatg caatgggagc gcgagattga
     7501 taactataca ggagttatct acaagctgat tgaggaatcc cagaaccaac aggagaaaaa
     7561 tgaacaagaa cttttggccc tggataaatg ggcaaatctg tggaactggt ttgacatcac
     7621 aagatggctt tggtacatta agatttttat tatgatcgta gggggtttgg tagggcttcg
     7681 gatcgttttt gctgtgctct ccatcgtgaa ccgagtcagg caggggtact cacctttgag
     7741 cctgcaaacg cactttcctg cgccaagagg accggatcga ccagagggaa cggaggagga
     7801 aggtggtgac cgggatcggg atcgcagcac tcccctcgtg gatgggttcc tttgataact
     7861 cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt ggacaaacca
     7921 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     7981 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8041 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     8101 gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     8161 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8221 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac
     8281 tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt
     8341 cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc
     8401 tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac
     8461 atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac
     8521 tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc
     8581 ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac
     8641 accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt
     8701 agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg
     8761 tagtagtata tactatccag actaacccta attcaatagc atatgttacc caacgggaag
     8821 catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc
     8881 catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt
     8941 ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc
     9001 gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta
     9061 aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg
     9121 acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg
     9181 ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat
     9241 ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc
     9301 aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac
     9361 caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc
     9421 cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac
     9481 gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg
     9541 gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa
     9601 taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca
     9661 ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga
     9721 ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg
     9781 ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc
     9841 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag
     9901 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     9961 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10021 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgt
//
