LOCUS       sequence-239627-       11797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160-V200E-754*
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(786..804)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        872..976
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(977..1045,1046..1837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1195..1214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2008..2596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2497..2516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3027..3172
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3215..4252
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4250..4269)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4294..4342
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4996..5375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5376..5579
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5530..5550
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5576..5600
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7213..7224
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    7930..8064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7954..7973)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8008..8027
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8207..8229)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8789..10578
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcctccactg cctcctcgac cccggcctcc wcctcctgct cctgcccctc ctgctcctgc
       61 ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact
      121 tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc
      181 cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt
      241 atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc
      301 cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac
      361 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
      421 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
      481 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
      541 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
      601 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
      661 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
      721 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
      781 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      841 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      901 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      961 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1021 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1081 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1141 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1201 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1261 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1321 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1381 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1441 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1501 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     1561 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1621 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1681 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1741 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1801 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1861 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1921 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1981 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2041 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2101 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2161 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2221 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2281 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2341 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2401 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2461 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2521 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2581 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2641 gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     2701 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     2761 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg
     2821 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     2881 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     2941 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
     3001 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
     3061 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
     3121 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
     3181 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     3241 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     3301 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     3361 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     3421 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     3481 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     3541 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     3601 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     3661 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     3721 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3781 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3841 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3901 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3961 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     4021 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     4081 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     4141 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     4201 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
     4261 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4321 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4381 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4441 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4501 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
     4561 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
     4621 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
     4681 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
     4741 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     4801 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     4861 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     4921 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4981 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     5041 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     5101 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     5161 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     5221 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     5281 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     5341 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     5401 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     5461 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     5521 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     5581 gtttagtgaa ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgccaa
     5641 tgggtagtct tcagcctctc gccaccttgt atctgctcgg tatgctggtt gccagcgttc
     5701 tggctaactt gtgggtgacc gtatactacg gggtcccggt atggaaggag gcaaacacta
     5761 cactcttctg tgcttccgac gcaaaggggt atgatacgga gaagcataat gtctgggcga
     5821 cacatgcatg tgtgcctaca gacccaaacc cgcaggaagt cctccttggt aataacgtga
     5881 ccgagaactt taatatgtgg aaaaataaca tggtagagca gatgcatgaa gacattataa
     5941 gcttgtggga tcaatctctt aagccgtgtg tgaagctgac accgctttgc gtaacactca
     6001 attgtacgga tttgacgaaa aatagcacta ccaataactc caccgctact aacaatacca
     6061 cgaacaatag taccgaaaaa atggagatgg acaaaggcga gatgaagaat tgctccttta
     6121 atatcacaac aaatattcga gataagatgc agaaagaata cgctttgctg tacaagcttg
     6181 acatcgtgcc gatcgacaat ggcaagaata acagcaacac caattatcgc ctgatttcat
     6241 gtaatacctc agagattacg caatcatgtc ccaaagtgtc atttcagccc attcctattc
     6301 attattgtgc acccgccggt tttgcaatac ttaagtgtaa taataagacc tttaatggaa
     6361 gcgggccttg tactaatgtg tcttccgtgc aatgcactca cggtatccga cctgttgttt
     6421 caacccagct tttgctgaac ggaagtctcg ctgaagaaga aatcgtgatt aggtccgcta
     6481 acttctctga caacacgaag actataattg tccaacttaa ggaagcggtc gaaataaact
     6541 gtacgcgccc gaataataac acgcgaaaat caattaacat cgggcctggg cgagcttttt
     6601 atgctactgg caacataata ggggacatcc gccaagctca ttgtaacata tcacggacaa
     6661 agtggaataa tacgttgaca gaaattgcta agaagctgaa caagcagtac gagaatagaa
     6721 caatagcatt taatcaatcc tccggaggcg atcttgagat cgtcatgcat tcattcaact
     6781 gcggtggaga atttttctat tgcaacactt cccagctgtt cagagatacg tggaacaaca
     6841 cttggaacgg catatggaac aacgatacgg aggtcaacga gactatcaca cttccgtgta
     6901 gaatcaaaca aataatcaat atgtggcaag aagtcgggaa ggcgatgtat gccccgccaa
     6961 tccgcggcca aatcaattgc tcatcaaata ttacgggact cctgctcacc cgcgacgggg
     7021 gagaaagcaa caagaccata gaggtcttcc ggcccggtgg cggagacatg agggacaact
     7081 ggaggtccga gctgtataag tacaaagttg ttaagataga gcccctcggg gttgctccca
     7141 ccaaagctaa aagaagagtg gtgcaacgag agaaaagagc cgtcggcttg ggtgccctct
     7201 tcgtcggatt tttgggagct gcaggtagca caatgggtgc tgcctctata acgctgaccg
     7261 tacaagctcg acagcttctt agtggtatcg ttcaacaaca aaacaatctc ttgcgagcaa
     7321 tagaagccca acagcatttg cttcaactga cagtatgggg cgttaagcag ttgcaagcgc
     7381 gggtcttggc ggtcgaacga tacctgaaag accagcaact cctgggcttg tggggatgca
     7441 gcggcaaact tatatgtacc acagctgtac cttggaacac cagctggagt aacaagagtt
     7501 acgattatat ctgggacaac atgacgtgga tgcaatggga gcgcgagatt gataactata
     7561 caggagttat ctacaagctg attgaggaat cccagaacca acaggagaaa aatgaacaag
     7621 aacttttggc cctggataaa tgggcaaatc tgtggaactg gtttgacatc acaagatggc
     7681 tttggtacat taagattttt attatgatcg tagggggttt ggtagggctt cggatcgttt
     7741 ttgctgtgct ctccatcgtg aaccgagtca ggcaggggta ctcacctttg agcctgcaaa
     7801 cgcactttcc tgcgccaaga ggaccggatc gaccagaggg aacggaggag gaaggtggtg
     7861 accgggatcg ggatcgcagc actcccctcg tggatgggtt cctttgataa ctcgaggccg
     7921 gcaaggccgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     7981 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     8041 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     8101 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     8161 gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     8221 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     8281 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgaggtcg actctagagg
     8341 atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc ttcttcgcgg
     8401 ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc cctgttccac
     8461 atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg acatccttat
     8521 aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag actttgcagt
     8581 ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc tcttacacca
     8641 atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct acaccaacgt
     8701 caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca ttagcaatag
     8761 tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg ggtagtagta
     8821 tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga agcatatgct
     8881 atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac cccatgagct
     8941 gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca ttttagtcac
     9001 aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct tcgcctgctt
     9061 cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag taaggtgtat
     9121 gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt ggacgggggg
     9181 ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct tgggcaataa
     9241 atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc atggggtggg
     9301 gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg gcaacacata
     9361 atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg accaagacag
     9421 gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac gccgacagca
     9481 gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc acgccaatgg
     9541 ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt gggggcacgc
     9601 gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt aataacttgg
     9661 ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac cactaatggc
     9721 accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc gattgctgcg
     9781 atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt tgttggtcct
     9841 catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta gcatatacta
     9901 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     9961 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
    10021 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
    10081 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
    10141 aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat atactaccca
    10201 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc tatcctaatc
    10261 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
    10321 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
    10381 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
    10441 tgtatccggg tagcatatgc tatcctcatg catatacagt cagcatatga tacccagtag
    10501 tagagtggga gtgctatcct ttgcatatgc cgccacctcc caagggggcg tgaattttcg
    10561 ctgcttgtcc ttttcctgct ggttgctccc attcttaggt gaatttaagg aggccaggct
    10621 aaagccgtcg catgtctgat tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag
    10681 aaggtgttga gcgcggagct gagtgacgtg acaacatggg tatgcccaat tgccccatgt
    10741 tgggaggacg aaaatggtga caagacagat ggccagaaat acaccaacag cacgcatgat
    10801 gtctactggg gatttattct ttagtgcggg ggaatacacg gcttttaata cgattgaggg
    10861 cgtctcctaa caagttacat cactcctgcc cttcctcacc ctcatctcca tcacctcctt
    10921 catctccgtc atctccgtca tcaccctccg cggcagcccc ttccaccata ggtggaaacc
    10981 agggaggcaa atctactcca tcgtcaaagc tgcacacagt caccctgata ttgcaggtag
    11041 gagcgggctt tgtcataaca aggtccttaa tcgcatcctt caaaacctca gcaaatatat
    11101 gagtttgtaa aaagaccatg aaataacaga caatggactc ccttagcggg ccaggttgtg
    11161 ggccgggtcc aggggccatt ccaaagggga gacgactcaa tggtgtaaga cgacattgtg
    11221 gaatagcaag ggcagttcct cgccttaggt tgtaaaggga ggtcttacta cctccatata
    11281 cgaacacacc ggcgacccaa gttccttcgt cggtagtcct ttctacgtga ctcctagcca
    11341 ggagagctct taaaccttct gcaatgttct caaatttcgg gttggaacct ccttgaccac
    11401 gatgctttcc aaaccaccct ccttttttgc gcctgcctcc atcaccctga ccccggggtc
    11461 cagtgcttgg gccttctcct gggtcatctg cggggccctg ctctatcgct cccgggggca
    11521 cgtcaggctc accatctggg ccaccttctt ggtggtattc aaaataatcg gcttccccta
    11581 cagggtggaa aaatggcctt ctacctggag ggggcctgcg cggtggagac ccggatgatg
    11641 atgactgact actgggactc ctgggcctct tttctccacg tccacgacct ctccccctgg
    11701 ctctttcacg acttcccccc ctggctcttt cacgtcctct accccggcgg cctccactac
    11761 ctcctcgacc ccggcctcca ctacctcctc gaccccg
//
