LOCUS       sequence-239658-       11642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422-QES.c12-754*
FEATURES             Location/Qualifiers
     source          1..11642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(738..756)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        824..928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(929..997,998..1789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1147..1166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1960..2548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2449..2468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2979..3124
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3167..4204
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4202..4221)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4246..4294
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4948..5327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5328..5531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5482..5502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5528..5552
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7834..7968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7858..7877)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7912..7931
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8111..8133)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8693..10482
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca
       61 gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga
      121 tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc
      181 tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc
      241 agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga
      301 catgattcac actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca
      361 gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc
      421 tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac
      481 caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct
      541 caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc
      601 gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag
      661 aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa
      721 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      781 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      841 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      901 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      961 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     1021 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1081 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1141 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     1201 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1261 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1321 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1381 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1441 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     1501 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1561 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1621 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1681 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1741 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1801 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1861 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1921 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1981 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2041 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2101 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2161 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2221 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2281 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2341 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2401 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2461 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2521 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2581 ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
     2641 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
     2701 catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg
     2761 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     2821 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     2881 ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
     2941 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
     3001 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca
     3061 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
     3121 ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga
     3181 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3241 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3301 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3361 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     3421 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3481 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     3541 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3601 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3661 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3721 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3781 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3841 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3901 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3961 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     4021 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4081 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4141 cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa
     4201 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4261 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     4321 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     4381 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     4441 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
     4501 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact
     4561 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
     4621 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
     4681 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc
     4741 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     4801 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     4861 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     4921 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
     4981 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     5041 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5101 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5161 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     5221 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     5281 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     5341 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     5401 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     5461 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     5521 taagcagagc tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagcg
     5581 ccaccatgcc catgggttcc ctccaaccac ttgcaacgct ttatctgctc ggcatgcttg
     5641 tagcgtcagt gcttgcaaat ctcgaccttt gggtcaccgt atactacgga gtccccgtgt
     5701 ggaaagaggc taagacgacc ctgttttgtg cgtcagacgc gaaagcgtat gacaaggaag
     5761 tgcataacgt gtgggcaaca cacgcatgtg ttccgacgga ccctaacccg caggaaatcg
     5821 tccttgaaaa cgtgaccgaa aacttcaaca tgtggaagaa cgacatggtt gaccaaatgc
     5881 acgaggacat aatcagtctt tgggatcaat ccttgaagcc ctgtgtgaag ctgacgcctt
     5941 tgtgcgtaac tttgaactgt aaaaacgtaa atatttcagc gaatgctaac gcgaccgcta
     6001 cacttaattc aagtatgaac ggcgaaataa agaattgctc cttcaacacc acgacagaac
     6061 ttagagataa aaagcaaaaa gtctacgccc ttttctataa accggatctc gtgccactta
     6121 acggtggtga gcacaatgag acaggtgagt acattctcat caactgcaat tcatctacaa
     6181 taacgcaagc atgtccgaaa atgtccttcg atccgatccc tattcactac tgcgctcctg
     6241 cagggtacgc catactgaag tgtaacaata aaacgtttaa cggaacaggc ccttgcaata
     6301 atgtaagtac tgtccagtgc acgcatggca taaaacccac catgagcaca caacttcttc
     6361 ttaacggatc actcgccgag gaagagatca tcgtgcgaag tgagaacttg acgaataata
     6421 taaagccgat catagtccat ctcaacaagt ccgtcgagat taaatgcact aggcccaaca
     6481 acaatacgcg gaaatccgta cggataggtc ctggacaaac attctatgct accggtgaga
     6541 ttataggtga tatacgcgaa gctcattgca atatcagtag ggagacctgg aattccactt
     6601 tgatccaagt gaaagaaaaa ctgcgggagc attacaacaa aacgatcaaa tttgaacctt
     6661 cttctggagg tgacctggag gttacgacgc acagttttaa ctgtagggga gaatggttct
     6721 actgcgatac gaccaagctg ttcaacgaga ctaagctgtt taacgagtca gaatacgttg
     6781 acaataagac tattatcttg ccctgccgga taaagcaaat tatcaatatg tggcaagaag
     6841 tgggaagggc aatgtacgcg ccgccgattg aagggaatat tacttgcaag agcaacataa
     6901 ctggtcttct tctcacatgg gatggtggag agaacagtac cgaaggcgtt tttaggcctg
     6961 gcggtggcaa tatgaaggat aactggcgct cagaactctg gaagtacaaa gtcgtcgaaa
     7021 taaagccctt gggcgttgcg cctacgaaaa gtaaacgaaa agtagtaggg agggaaaagc
     7081 gagcggttgg cttgggtgca gtgcttctcg ggttcctggg agctgcgggg tcaacaatgg
     7141 gagctgcttc cataaccttg actgtgcagg caaggcagct gctgagtggg atagtgcagc
     7201 aacaatccaa tctgcttagg gcaattgaag ctcagcaaca tcttttgcag ctcacagtct
     7261 ggggcataaa gcaactgcaa accagagtcc tcgcgattga gcggtatctt aaggaccagc
     7321 aactgctggg actctggggg tgttccggga agcttatttg tgccactgcg gtaccatgga
     7381 actcctcttg gtccaacaag tctctgggcg atatttggga caatatgacg tggatgcagt
     7441 gggatcgcga gatatccaac tacaccaaca cgatcttccg gcttctcgaa gacagtcaga
     7501 atcaacaaga gaagaatgag aaggatctgt tggcccttga cagctggaaa aatctttgga
     7561 attggtttga cattactaac tggctgtggt acataaaaat cttcataatg attgtcggtg
     7621 gccttatcgg tctccgcatc atattcgggg tattggctat agtcaaaagg gtacgccagg
     7681 ggtatagccc gttgagtttc caaacgctca ttccgaatcc gcgaggtcct gatcgcttgg
     7741 gacggatcga agaggaagga ggtgaacaag acaaggatcg atccatacga ctggtctctg
     7801 ggtttcttta gtaactcgag gccggcaagg ccggatccag acatgataag atacattgat
     7861 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     7921 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     7981 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     8041 aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg
     8101 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8161 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     8221 cagccatgag gtcgactcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     8281 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     8341 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     8401 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     8461 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     8521 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     8581 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     8641 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     8701 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     8761 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     8821 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     8881 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     8941 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     9001 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     9061 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     9121 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     9181 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     9241 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     9301 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     9361 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     9421 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     9481 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     9541 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     9601 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     9661 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     9721 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     9781 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     9841 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     9901 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     9961 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
    10021 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
    10081 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
    10141 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
    10201 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
    10261 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
    10321 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata
    10381 cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac
    10441 ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt
    10501 aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat
    10561 gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca
    10621 tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag
    10681 aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata
    10741 cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct
    10801 caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag
    10861 ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca
    10921 cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat
    10981 ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg
    11041 actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac
    11101 tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa
    11161 gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag
    11221 tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt
    11281 tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc
    11341 ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc
    11401 cctgctctat cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt
    11461 attcaaaata atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc
    11521 tgcgcggtgg agacccggat gatgatgact gactactggg actcctgggc ctcttttctc
    11581 cacgtccacg acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc
    11641 ct
//
