LOCUS       sequence-239663-       11911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422-QES.c12
FEATURES             Location/Qualifiers
     source          1..11911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(726..744)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        812..916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(917..985,986..1777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1135..1154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1948..2536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2437..2456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2967..3112
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3155..4192
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4190..4209)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4234..4282
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4936..5315
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5316..5519
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5470..5490
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5516..5540
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8152..8286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8176..8195)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8230..8249
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8429..8451)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9011..10800
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact
       61 tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc
      121 cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt
      181 atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc
      241 cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac
      301 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
      361 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
      421 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
      481 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
      541 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
      601 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
      661 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg
      721 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      781 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1141 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1441 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     1501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2581 gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     2641 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     2701 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg
     2761 ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     2821 gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     2881 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
     2941 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
     3001 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
     3061 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
     3121 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
     3181 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     3241 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     3301 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     3361 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     3421 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     3481 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     3541 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     3601 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     3661 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     3721 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     3781 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     3841 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     3901 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     3961 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     4021 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     4081 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     4141 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
     4201 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4261 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4321 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4381 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4441 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
     4501 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
     4561 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
     4621 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
     4681 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     4741 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     4801 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     4861 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     4921 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4981 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     5041 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     5101 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     5161 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     5221 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     5281 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     5341 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     5401 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     5461 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     5521 gtttagtgaa ccgtcagatc tctagaagct gggtaccagc tgctagcgcc accatgccca
     5581 tgggttccct ccaaccactt gcaacgcttt atctgctcgg catgcttgta gcgtcagtgc
     5641 ttgcaaatct cgacctttgg gtcaccgtat actacggagt ccccgtgtgg aaagaggcta
     5701 agacgaccct gttttgtgcg tcagacgcga aagcgtatga caaggaagtg cataacgtgt
     5761 gggcaacaca cgcatgtgtt ccgacggacc ctaacccgca ggaaatcgtc cttgaaaacg
     5821 tgaccgaaaa cttcaacatg tggaagaacg acatggttga ccaaatgcac gaggacataa
     5881 tcagtctttg ggatcaatcc ttgaagccct gtgtgaagct gacgcctttg tgcgtaactt
     5941 tgaactgtaa aaacgtaaat atttcagcga atgctaacgc gaccgctaca cttaattcaa
     6001 gtatgaacgg cgaaataaag aattgctcct tcaacaccac gacagaactt agagataaaa
     6061 agcaaaaagt ctacgccctt ttctataaac cggatctcgt gccacttaac ggtggtgagc
     6121 acaatgagac aggtgagtac attctcatca actgcaattc atctacaata acgcaagcat
     6181 gtccgaaaat gtccttcgat ccgatcccta ttcactactg cgctcctgca gggtacgcca
     6241 tactgaagtg taacaataaa acgtttaacg gaacaggccc ttgcaataat gtaagtactg
     6301 tccagtgcac gcatggcata aaacccacca tgagcacaca acttcttctt aacggatcac
     6361 tcgccgagga agagatcatc gtgcgaagtg agaacttgac gaataatata aagccgatca
     6421 tagtccatct caacaagtcc gtcgagatta aatgcactag gcccaacaac aatacgcgga
     6481 aatccgtacg gataggtcct ggacaaacat tctatgctac cggtgagatt ataggtgata
     6541 tacgcgaagc tcattgcaat atcagtaggg agacctggaa ttccactttg atccaagtga
     6601 aagaaaaact gcgggagcat tacaacaaaa cgatcaaatt tgaaccttct tctggaggtg
     6661 acctggaggt tacgacgcac agttttaact gtaggggaga atggttctac tgcgatacga
     6721 ccaagctgtt caacgagact aagctgttta acgagtcaga atacgttgac aataagacta
     6781 ttatcttgcc ctgccggata aagcaaatta tcaatatgtg gcaagaagtg ggaagggcaa
     6841 tgtacgcgcc gccgattgaa gggaatatta cttgcaagag caacataact ggtcttcttc
     6901 tcacatggga tggtggagag aacagtaccg aaggcgtttt taggcctggc ggtggcaata
     6961 tgaaggataa ctggcgctca gaactctgga agtacaaagt cgtcgaaata aagcccttgg
     7021 gcgttgcgcc tacgaaaagt aaacgaaaag tagtagggag ggaaaagcga gcggttggct
     7081 tgggtgcagt gcttctcggg ttcctgggag ctgcggggtc aacaatggga gctgcttcca
     7141 taaccttgac tgtgcaggca aggcagctgc tgagtgggat agtgcagcaa caatccaatc
     7201 tgcttagggc aattgaagct cagcaacatc ttttgcagct cacagtctgg ggcataaagc
     7261 aactgcaaac cagagtcctc gcgattgagc ggtatcttaa ggaccagcaa ctgctgggac
     7321 tctgggggtg ttccgggaag cttatttgtg ccactgcggt accatggaac tcctcttggt
     7381 ccaacaagtc tctgggcgat atttgggaca atatgacgtg gatgcagtgg gatcgcgaga
     7441 tatccaacta caccaacacg atcttccggc ttctcgaaga cagtcagaat caacaagaga
     7501 agaatgagaa ggatctgttg gcccttgaca gctggaaaaa tctttggaat tggtttgaca
     7561 ttactaactg gctgtggtac ataaaaatct tcataatgat tgtcggtggc cttatcggtc
     7621 tccgcatcat attcggggta ttggctatag tcaaaagggt acgccagggg tatagcccgt
     7681 tgagtttcca aacgctcatt ccgaatccgc gaggtcctga tcgcttggga cggatcgaag
     7741 aggaaggagg tgaacaagac aaggatcgat ccatacgact ggtctctggg tttcttgccc
     7801 tcgcgtggga cgacctcaga tcactgtgcc tgttctctta tcatcaattg agggacttca
     7861 tactgaccgc agcaagagcc gcagagctgc tgggtcgcag ttccctgcga ggtcttcagc
     7921 gaggttggga agtactcaag tacctcggaa atctggtcca atactggggt ctcgaactga
     7981 agcgatccgc gataaacctc tttgacacaa tagcaattgc ggtggccgag ggaacggacc
     8041 gaataataga agtcatacag cgcatttgca gggctatccg atatatccca acccgaattc
     8101 gacaaggttt cgaagccgca ttgttgtagt aactcgaggc cggcaaggcc ggatccagac
     8161 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     8221 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     8281 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     8341 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tccggctgcc
     8401 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     8461 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     8521 ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc ccgccccgga
     8581 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     8641 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     8701 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     8761 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     8821 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     8881 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     8941 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     9001 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     9061 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     9121 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     9181 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     9241 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     9301 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     9361 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     9421 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     9481 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     9541 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     9601 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     9661 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     9721 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     9781 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     9841 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     9901 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     9961 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
    10021 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
    10081 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
    10141 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
    10201 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
    10261 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    10321 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
    10381 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
    10441 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
    10501 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    10561 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    10621 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
    10681 gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg gagtgctatc
    10741 ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg
    10801 ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg
    10861 attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag
    10921 ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga cgaaaatggt
    10981 gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg gggatttatt
    11041 ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct aacaagttac
    11101 atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg tcatctccgt
    11161 catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc aaatctactc
    11221 catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc tttgtcataa
    11281 caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt aaaaagacca
    11341 tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt ccaggggcca
    11401 ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca agggcagttc
    11461 ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca ccggcgaccc
    11521 aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct cttaaacctt
    11581 ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt ccaaaccacc
    11641 ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt gggccttctc
    11701 ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc tcaccatctg
    11761 ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg aaaaatggcc
    11821 ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga ctactgggac
    11881 tcctgggcct cttttctcca cgtccacgac c
//
