LOCUS       sequence-239667-       11522 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11522)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH111gp160-754*
FEATURES             Location/Qualifiers
     source          1..11522
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(715..733)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        801..905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(906..974,975..1766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1124..1143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2426..2445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2956..3101
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3144..4181
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4179..4198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4223..4271
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4925..5304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5305..5508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5459..5479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5505..5529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7829..7963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7853..7872)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7907..7926
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8106..8128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8688..10477
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt
       61 ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc ggggctcctg
      121 gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta tcaccccctc
      181 ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc ttctctccta
      241 ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact aaaagagatc
      301 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
      361 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
      421 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
      481 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
      541 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
      601 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
      661 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     2581 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     2641 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     2701 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     2761 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     2821 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     2881 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     2941 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     3001 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     3061 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     3121 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     3181 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     3241 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3301 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3361 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     3421 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     3481 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3541 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3601 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3661 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3721 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3781 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3841 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3901 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     3961 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     4021 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     4081 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     4141 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     4201 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     4261 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4321 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4381 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     4441 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     4501 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     4561 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     4621 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
     4681 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     4741 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     4801 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     4861 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     4921 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4981 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5041 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5101 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5161 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     5221 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     5281 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     5341 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5401 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     5461 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     5521 cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcccat gggtagtctg
     5581 cagcctttgg ctactcttta cttgttgggt atgttggtag cgagcgtcct cgcagtcggg
     5641 aatttgtggg ttacagttta ttacggggtc ccggtgtgga aggaggcgac gaccacattg
     5701 ttttgcgcct ccgatgcaaa ggcttacgac acggaagttc acaacgtttg ggccacccat
     5761 gcgtgcgtgc ctacggaccc taatccacag gagatgttca tgcccaatgt caccgaaaat
     5821 tttaatatgt ggaaaaacga catggtaaat caaatgcatg aggatgtcat aagtctctgg
     5881 gaccaaagtc tgaagccatg cgtgaaattg accccgctct gcgtgacact ggagtgtaaa
     5941 aacgtgtcac gaaataattc cagttcaaat ggtactggta caaacaatga agcgtatcga
     6001 gagggaatga aggaaataaa gaactgctct tttaatgcca ctgccgagct tagagaccgg
     6061 aagcagaacg tgtatgcact gttttacagg cttgacatcg tacctcttgc ggagaacagt
     6121 agcgaatata ggcttatcaa ttgcaacacc agcgcaatta ctcaagcctg ccccaaagtg
     6181 acttttgacc cgattcctat acactactgc acaccagcag ggtacgcaat tcttaagtgt
     6241 agcgacaaaa ccttcaacgg cactggcccc tgttcaaatg tctccacagt tcaatgtact
     6301 cacggaatac gaccggtcgt aagtacacag ttgctgctga acggttctct ggccgaagag
     6361 gagatcatca tccgctctga gaacctcacg aacaatgcca agacaataat cgtacagctt
     6421 aaccagagcg tggaaatagt ttgcacacgc cctaacaata acactagaaa gtcaatcagg
     6481 atcggaccag ggcagacgtt ttatgcgaca ggaggaataa taggcgacat ccgacaagcg
     6541 tactgtaaca tttcccggaa taaatggaac gagacattga gaatggtagg taaaaaattg
     6601 gcggaacatt ttcctaacaa aaccatcaat tttacatcct cctccggggg agatctggaa
     6661 atcacgacgc acagttttac gtgtagggga gaattcttct attgcaacac ctccggactg
     6721 tttaatggta cgtacatgcc caacggtacg tatatgttca actctacgaa tgccacgatc
     6781 acaatcccgt gtagaattaa gcagatcatc aatatgtggc aaggggtcgg ccgagccata
     6841 tatgctccgc ctatagcggg caatatcacc tgcaaatcca acataacggg tcttcttctt
     6901 gtacgcgacg gtggagaaac aaacgatacc aataccaccg agacatttag accaggcgga
     6961 ggagatatgc gagataattg gcggagcgaa ttgtacaagt acaaggtggt cgagataaag
     7021 cctttgggtg tggcacccac gggcgcaaag cgcagaatag tcggaagaga aaagagagca
     7081 gttgggctcg gcgctgcttt tcttggtttc ctcagcgttg ccggtagtac gatgggagca
     7141 gcgtcaatga ccctgactgt ccaagcgcga cagctcctta gcggaatagt gcaacaacag
     7201 agcaacctgc tgcgggcaat tgaagcacag cagcatttgc tccaactgac tgtctggggt
     7261 ataaagcaac tgcaggccag ggttttggcc atagagagat acctcaaaga tcaacagctc
     7321 ctgggtatct gggggtgttc aggaaagctc atctgtacga ccacagtacc ctggaactct
     7381 tcctggagca ataaaaacct tactgagatc tgggataata tgacttggat gcagtgggac
     7441 aaggaaatct ccaactacac caatacgatt tattggttgc ttgaggagtc acagaatcag
     7501 caagagaaga acgaaaagga tctcctcgcc ctcgattcat ggaacaattt gtggaactgg
     7561 ttcgatattt ctaaatggct gtggtacatc aagatattca taatgatcgt cggcggactt
     7621 atcggtctcc gaatcatatt tgcggtactc agtatcgtga acagggtacg gcaagggtac
     7681 agtcccctta gctttcaaac acttaccccg aaccctggcg gacctgacag gcttggacga
     7741 atcgaggagg aaggaggaga acaagatcgc cgcaggtcta ttagactggt atccggcttc
     7801 ctctgataac tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt
     7861 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7921 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     7981 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     8041 ctacaaatgt ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt
     8101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     8161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     8221 atgaggtcga ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat
     8281 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     8341 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     8401 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     8461 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     8521 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     8581 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     8641 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     8701 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     8761 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     8821 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     8881 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     8941 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     9001 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     9061 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     9121 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     9181 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     9241 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     9301 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     9361 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     9421 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     9481 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     9541 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     9601 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     9661 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     9721 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     9781 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     9841 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     9901 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     9961 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
    10021 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
    10081 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
    10141 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
    10201 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
    10261 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    10321 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc
    10381 agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc
    10441 aagggggcgt gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg
    10501 aatttaagga ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc
    10561 taatgttttc caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt
    10621 atgcccaatt gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata
    10681 caccaacagc acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg
    10741 cttttaatac gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc
    10801 tcatctccat cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct
    10861 tccaccatag gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc
    10921 accctgatat tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc
    10981 aaaacctcag caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc
    11041 cttagcgggc caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat
    11101 ggtgtaagac gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag
    11161 gtcttactac ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt
    11221 tctacgtgac tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg
    11281 ttggaacctc cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca
    11341 tcaccctgac cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc
    11401 tctatcgctc ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca
    11461 aaataatcgg cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc
    11521 gg
//
