LOCUS       sequence-239671-       11580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; 
            QES mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH111-QES.c13.L544Y
FEATURES             Location/Qualifiers
     source          1..11580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(591..609)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        677..781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(782..850,851..1642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1000..1019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1813..2401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2302..2321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2832..2977
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3020..4057
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4055..4074)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4099..4147
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4801..5180
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5181..5384
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5335..5355
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5381..5405
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    8014..8148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8038..8057)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8092..8111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8291..8313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8873..10662
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct
       61 ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc
      121 atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata
      181 gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa
      241 cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt
      301 ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc
      361 gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc
      421 cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt
      481 caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc
      541 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
      601 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      781 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      841 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      901 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      961 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1021 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1081 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1141 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1201 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1261 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1321 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1381 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1441 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1501 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1561 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1621 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1681 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1921 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     1981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc
     2461 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     2521 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     2581 caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg
     2641 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     2701 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc
     2761 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     2821 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     2881 agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg
     2941 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
     3001 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3061 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3121 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3181 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3241 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3301 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3361 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     3421 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     3481 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     3541 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3601 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3661 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3721 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3781 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3841 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     3901 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     3961 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4021 cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg
     4081 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     4141 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4201 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4261 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
     4321 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
     4381 ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt
     4441 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
     4501 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
     4561 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     4621 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     4681 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     4741 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4801 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4861 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4921 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4981 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5041 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5101 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     5161 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     5221 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5281 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     5341 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     5401 agatctctag aagctgggta ccagctgcta gcgccaccat gcccatgggt agtctgcagc
     5461 ctttggctac tctttacttg ttgggtatgt tggtagcgag cgtcctcgca gtcgggaatt
     5521 tgtgggttac agtttattac ggggtcccgg tgtggaagga ggcgacgacc acattgtttt
     5581 gcgcctccga tgcaaaggct tacgacacgg aagttcacaa cgtttgggcc acccatgcgt
     5641 gcgtgcctac ggaccctaat ccacaggaga tgttcatgcc caatgtcacc gaaaatttta
     5701 atatgtggaa aaacgacatg gtaaatcaaa tgcatgagga tgtcataagt ctctgggacc
     5761 aaagtctgaa gccatgcgtg aaattgaccc cgctctgcgt gacactggag tgtaaaaacg
     5821 tgtcacgaaa taattccagt tcaaatggta ctggtacaaa caatgaagcg tatcgagagg
     5881 gaatgaagga aataaagaac tgctctttta atgccactgc cgagcttaga gaccggaagc
     5941 agaacgtgta tgcactgttt tacaggcttg acatcgtacc tcttgcggag aacagtagcg
     6001 aatataggct tatcaattgc aacaccagcg caattactca agcctgcccc aaaatgactt
     6061 ttgacccgat tcctatacac tactgcacac cagcagggta cgcaattctt aagtgtagcg
     6121 acaaaacctt caacggcact ggcccctgtt caaatgtctc cacagttcaa tgtactcacg
     6181 gaatacgacc ggtcatgagt acacagttgc tgctgaacgg ttctctggcc gaagaggaga
     6241 tcatcatccg ctctgagaac ctcacgaaca atgccaagcc aataatcgta cagcttaacc
     6301 agagcgtgga aatagtttgc acacgcccta acaataacac tagaaagtca atcaggatcg
     6361 gaccagggca gacgttttat gcgacaggag gaataatagg cgacatccga caagcgtact
     6421 gtaacatttc ccggaataaa tggaacgaga cattgagaat ggtaggtaaa aaattggcgg
     6481 aacattttcc taacaaaacc atcaatttta catcctcctc cgggggagat ctggaaatca
     6541 cgacgcacag ttttacgtgt aggggagaat tcttctattg caacacctcc ggactgttta
     6601 atggtacgta catgcccaac ggtacgtata tgttcaactc tacgaatgcc acgatcacaa
     6661 tcccgtgtag aattaagcag atcatcaata tgtggcaagg ggtcggccga gccatatatg
     6721 ctccgcctat agcgggcaat atcacctgca aatccaacat aacgggtctt cttcttgtac
     6781 gcgacggtgg agaaacaaac gataccaata ccaccgagac atttagacca ggcggaggag
     6841 atatgcgaga taattggcgg agcgaattgt acaagtacaa ggtggtcgag ataaagcctt
     6901 tgggtgtggc acccacgggc gcaaagcgca gaatagtcgg aagagaaaag agagcagttg
     6961 ggctcggcgc tgcttttctt ggtttcctca gcgttgccgg tagtacgatg ggagcagcgt
     7021 caatgaccct gactgtccaa gcgcgacagt accttagcgg aatagtgcaa caacagagca
     7081 acctgctgcg ggcaattgaa gcacagcagc atttgctcca actgactgtc tggggtataa
     7141 agcaactgca ggccagggtt ttggccatag agagatacct caaagatcaa cagctcctgg
     7201 gtatctgggg gtgttcagga aagctcatct gtacgaccac agtaccctgg aactcttcct
     7261 ggagcaataa aaaccttact gagatctggg ataatatgac ttggatgcag tgggacaagg
     7321 aaatctccaa ctacaccaat acgatttatt ggttgcttga ggagtcacag aatcagcaag
     7381 agaagaacga aaaggatctc ctcgccctcg attcatggaa caatttgtgg aactggttcg
     7441 atatttctaa atggctgtgg tacatcaaga tattcataat gatcgtcggc ggacttatcg
     7501 gtctccgaat catatttgcg gtactcagta tcgtgaacag ggtacggcaa gggtacagtc
     7561 cccttagctt tcaaacactt accccgaacc ctggcggacc tgacaggctt ggacgaatcg
     7621 aggaggaagg aggagaacaa gatcgccgca ggtctattag actggtatcc ggcttcctcg
     7681 cgcttgcgtg ggacgatttg cgaaacttgt gtttgtttag ttatcatcag ttgagggatt
     7741 tcatcctcgt cgcggcgcgc gtcgtcgagc ttttgggtca aagaggatgg gaagcgctca
     7801 agtacctcgg cagtttggta caatattggg ggcaagaatt gaaaaaatct gctatctcac
     7861 tgattgatac tattgccata gccgttgctg aaggaactga tcggattata gagttgatcc
     7921 agggcctttg ccgcgctata taccatattc cacgaaggat ccggcaggga tttgaggcag
     7981 cgttgcaata gtaactcgag gccggcaagg ccggatccag acatgataag atacattgat
     8041 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     8101 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     8161 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     8221 aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg
     8281 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     8341 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     8401 cagccatgag gtcgactcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     8461 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     8521 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     8581 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     8641 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     8701 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     8761 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     8821 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     8881 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     8941 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     9001 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     9061 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     9121 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     9181 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     9241 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     9301 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     9361 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     9421 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     9481 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     9541 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     9601 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     9661 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     9721 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     9781 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     9841 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     9901 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     9961 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
    10021 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
    10081 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
    10141 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
    10201 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
    10261 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
    10321 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
    10381 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
    10441 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
    10501 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata
    10561 cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac
    10621 ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt
    10681 aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat
    10741 gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca
    10801 tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag
    10861 aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata
    10921 cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct
    10981 caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag
    11041 ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca
    11101 cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat
    11161 ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg
    11221 actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac
    11281 tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa
    11341 gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag
    11401 tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt
    11461 tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc
    11521 ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc
//
