LOCUS       sequence-239672-       11407 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the SHIV327C-Hu A10 
            strain; QES mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-SHIV327C-HuA10-QES.i09.c07
FEATURES             Location/Qualifiers
     source          1..11407
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(415..433)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        501..605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(606..674,675..1466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(824..843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1637..2225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2126..2145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2656..2801
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2844..3881
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(3879..3898)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3923..3971
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4625..5004
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5005..5208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5159..5179
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5205..5229
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    7511..7645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7535..7554)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7589..7608
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7788..7810)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8370..10159
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact cctgccccct
       61 ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc caggtctgaa
      121 aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac
      181 tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg
      241 tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc ttcctcttca
      301 aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc ggcctgtgag
      361 gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg
      421 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      601 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      661 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      721 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      781 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      841 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      901 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      961 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1021 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1081 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1141 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1201 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1261 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1321 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1381 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1441 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1501 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1561 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1621 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1681 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1741 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1801 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1861 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1921 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1981 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2041 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2101 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2161 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2221 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttgaagct
     2281 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
     2341 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
     2401 ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat
     2461 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     2521 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc
     2581 atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat atatttgcat
     2641 gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc gaattcgaac
     2701 acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt
     2761 gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa cagcgtgccg
     2821 cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg tctgtcgaga
     2881 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     2941 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     3001 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     3061 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     3121 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     3181 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     3241 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     3301 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     3361 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     3421 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     3481 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     3541 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     3601 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     3661 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     3721 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     3781 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     3841 ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa ggagacaata
     3901 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     3961 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     4021 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4081 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
     4141 tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     4201 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     4261 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     4321 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
     4381 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     4441 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     4501 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     4561 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     4621 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4681 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4741 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4801 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4861 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     4921 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     4981 gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga
     5041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     5101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg
     5161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     5221 cgtcagatct ctagaagctg ggtaccagct gctagcgcca ccatgcctat gggttctttg
     5281 cagcccctgg ccacgctgta cctgctcggc atgctggttg cctcagtatt ggcgaacttg
     5341 tgggttacag tgtattacgg cgttccagtg tggaaagagg caaaaactac actcttctgt
     5401 gcgagcgatg ccaaagcata tgaacaggaa gttcacaatg tatgggcaac tcatgcatgc
     5461 gtgccgacag accctaaccc ccaagagatg cggatggaaa atgttacgga aaacttcaac
     5521 atgtggaaga atgatatggt cgaccagatg cacgaggata taattagctt gtgggatcag
     5581 tcccttaagc cctgcgtgaa acttacccca ctttgcgtta cattggattg ccaaaatgtc
     5641 aatgccactc aaaataccaa tgacacaata tccaccatga aaaattgcac cttcaacaca
     5701 acagcagatt tgggagataa aaagcagaaa gggagagcgc tcttttacaa cttggacatc
     5761 gtccaactca atcctaattc taattcatct gagtacaggc tgatctcctg caatacatcc
     5821 acaatcaccc aggcctgccc caagatgtct tttgaaccca tccccataca ctactgcgcg
     5881 cccgccgggt atgctattct taaatgcaac aacaagacct ttaatggcct cggcccttgc
     5941 acaaacgtca gcacagtaca gtgtacccat ggcataaaac cagtgatgtc tacacaattg
     6001 ctcctcaacg gttctcttgc tgagggagaa attatcataa ggtccgagaa tttgaccgat
     6061 aacggtaaaa caattatcgt tcatcttaac gaaagcgtga aaatagtgtg tattcgccca
     6121 aacaataaca cccggaagag catcaggata gggccgggac agacattctt tgcaacagac
     6181 atcataggag acatcaggca agcctattgc aacatcagca gggaggactg gaataaaaca
     6241 ctggacagag tacgaaaaaa actggaggag cacttcccca acaaaactat tgaatttaag
     6301 aggcattctg gcggtgatct tgaagtgacg cagcacagtt tcaactgtcg cggagagtgg
     6361 ttttattgta acactacgca cctttttgaa aatacgactt ataccaacag tagtaacatc
     6421 actttgccgt gcagaattaa gcaaataatc aatatgtggc agggagtcgg cagagctatg
     6481 tatgcccctc ctatcgctgg gaacataacc tgtatatcta atatcaccgg tattctcctc
     6541 acaagagatg gggggaataa cggaacaaat gagactttta gaccgggagg aggggacatg
     6601 cgagacaatt ggaggagcga attgtacaag tataaagtag tggaaattaa gccacttggc
     6661 atagctccga ccaaggcgaa gcggagagtt gttgagagag agaaaagagc cgtcggaatg
     6721 ggagcgctgt ttctgggatt tctcggagct gctggttcaa ccatgggggc tgcgtctatg
     6781 accctgacag ttcaagctcg gcagtacttg tccggtatcg tccaacaaca gagtaacttg
     6841 ctgcgcgcca tagaggcgca acaacacatg ttgcagctta cggtctgggg gattaagcaa
     6901 ctgcaggcgc gcgtactcgc actggagaga tacttgcaag atcagcagct gttgggaatg
     6961 tggggatgtt caggcaaact gatctgtact acagcagttc cttggaatag ttcctggagc
     7021 aacaagacat acaatgacat atgggataac atgacgtgga tgcagtggga aagagaaatc
     7081 agcaactata cgaatacgat atacaccttg ctcgaagtat cccaaaatca acaagagcaa
     7141 aacgagaaag atttgctcgc tctggacaag tggcaaaacc tttggagttg gtttaacata
     7201 accaactggc tgtggtacat caaaatattt attatgatag taggtggttt gataggcctc
     7261 aggattatct tcgcagtcct cagcattatt aatagggtga ggcaaggtta ctctccattg
     7321 tcttttcaga ctctcacccc gaatcccaga ggaccggatc gactcggggg aatagaggaa
     7381 gaaggtgggg agcaagaccg cgatcgaagt attagactgg ttagcgggtt cctctctttg
     7441 gcttgggacg acctcagaag tctctgtctc ttctcttacc acagatagta actcgaggcc
     7501 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     7561 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     7621 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     7681 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     7741 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7801 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7861 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag
     7921 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     7981 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     8041 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     8101 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     8161 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     8221 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     8281 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     8341 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     8401 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     8461 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     8521 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     8581 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     8641 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     8701 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     8761 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     8821 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     8881 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     8941 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     9001 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     9061 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     9121 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     9181 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     9241 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     9301 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     9361 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     9421 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     9481 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     9541 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     9601 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     9661 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     9721 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     9781 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9841 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     9901 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     9961 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
    10021 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
    10081 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
    10141 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
    10201 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
    10261 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
    10321 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
    10381 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
    10441 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
    10501 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
    10561 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
    10621 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
    10681 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
    10741 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
    10801 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
    10861 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
    10921 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
    10981 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
    11041 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
    11101 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
    11161 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
    11221 gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg
    11281 gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta
    11341 cctcctcgac cccggcctcc actacctcct cgaccccggc ctccactgcc tcctcgaccc
    11401 cggcctc
//
