LOCUS       sequence-239674-       11477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the SHIV327C-Hu A10 
            strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-SHIV327C-HuA10-gp160
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      1003..2792
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     3352..3374
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3517..3651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3554..3573)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3608..3627
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5933..5957)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5954..6157)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5983..6003)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6158..6537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7191..7239
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7264..7283
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7281..8318)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(8361..8506)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(8937..9525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9017..9036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9696..10487,10488..10556))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10319..10338
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10557..10661)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10729..10747
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg
       61 tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg
      121 gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc
      181 gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc
      241 tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc ggtgtgttcg
      301 tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc cttgctattc
      361 cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct ggacccggcc
      421 cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt ttacaaactc
      481 atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca aagcccgctc
      541 ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat ttgcctccct
      601 ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat gacggagatg
      661 aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg ttaggagacg
      721 ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc cccagtagac
      781 atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt cgtcctccca
      841 acatggggca attgggcata cccatgttgt cacgtcactc agctccgcgc tcaacacctt
      901 ctcgcgttgg aaaacattag cgacatttac ctggtgagca atcagacatg cgacggcttt
      961 agcctggcct ccttaaattc acctaagaat gggagcaacc agcaggaaaa ggacaagcag
     1021 cgaaaattca cgcccccttg ggaggtggcg gcatatgcaa aggatagcac tcccactcta
     1081 ctactgggta tcatatgctg actgtatatg catgaggata gcatatgcta cccggataca
     1141 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     1201 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
     1261 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     1321 gattaggata gcatatgcta cccagatata gattaggata gcatatgcta tccagatatt
     1381 tgggtagtat atgctaccca gatataaatt aggatagcat atactaccct aatctctatt
     1441 aggatagcat atgctacccg gatacagatt aggatagcat atactaccca gatatagatt
     1501 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt
     1561 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     1621 aggatagcct atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg
     1681 tagtatatgc tacccatggc aacattagcc caccgtgctc tcagcgacct cgtgaatatg
     1741 aggaccaaca accctgtgct tggcgctcag gcgcaagtgt gtgtaatttg tcctccagat
     1801 cgcagcaatc gcgcccctat cttggcccgc ccacctactt atgcaggtat tccccggggt
     1861 gccattagtg gttttgtggg caagtggttt gaccgcagtg gttagcgggg ttacaatcag
     1921 ccaagttatt acacccttat tttacagtcc aaaaccgcag ggcggcgtgt gggggctgac
     1981 gcgtgccccc actccacaat ttcaaaaaaa agagtggcca cttgtctttg tttatgggcc
     2041 ccattggcgt ggagccccgt ttaattttcg ggggtgttag agacaaccag tggagtccgc
     2101 tgctgtcggc gtccactctc tttccccttg ttacaaatag agtgtaacaa catggttcac
     2161 ctgtcttggt ccctgcctgg gacacatctt aataacccca gtatcatatt gcactaggat
     2221 tatgtgttgc ccatagccat aaattcgtgt gagatggaca tccagtcttt acggcttgtc
     2281 cccaccccat ggatttctat tgttaaagat attcagaatg tttcattcct acactagtat
     2341 ttattgccca aggggtttgt gagggttata ttggtgtcat agcacaatgc caccactgaa
     2401 ccccccgtcc aaattttatt ctgggggcgt cacctgaaac cttgttttcg agcacctcac
     2461 atacacctta ctgttcacaa ctcagcagtt attctattag ctaaacgaag gagaatgaag
     2521 aagcaggcga agattcagga gagttcactg cccgctcctt gatcttcagc cactgccctt
     2581 gtgactaaaa tggttcacta ccctcgtgga atcctgaccc catgtaaata aaaccgtgac
     2641 agctcatggg gtgggagata tcgctgttcc ttaggaccct tttactaacc ctaattcgat
     2701 agcatatgct tcccgttggg taacatatgc tattgaatta gggttagtct ggatagtata
     2761 tactactacc cgggaagcat atgctacccg tttagggtta acaagggggc cttataaaca
     2821 ctattgctaa tgccctcttg agggtccgct tatcggtagc tacacaggcc cctctgattg
     2881 acgttggtgt agcctcccgt agtcttcctg ggcccctggg aggtacatgt cccccagcat
     2941 tggtgtaaga gcttcagcca agagttacac ataaaggcaa tgttgtgttg cagtccacag
     3001 actgcaaagt ctgctccagg atgaaagcca ctcagtgttg gcaaatgtgc acatccattt
     3061 ataaggatgt caactacagt cagagaaccc ctttgtgttt ggtccccccc cgtgtcacat
     3121 gtggaacagg gcccagttgg caagttgtac caaccaactg aagggattac atgcactgcc
     3181 ccgcgaagaa ggggcagaga tgtcgtagtc aggtttagtt cgtccggggc ggggatcgat
     3241 cctctagagt cgacctcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     3301 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3361 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
     3421 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3481 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3541 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3601 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccggccttgc
     3661 cggcctcgag ttactatctg tggtaagaga agagacagag acttctgagg tcgtcccaag
     3721 ccaaagagag gaacccgcta accagtctaa tacttcgatc gcggtcttgc tccccacctt
     3781 cttcctctat tcccccgagt cgatccggtc ctctgggatt cggggtgaga gtctgaaaag
     3841 acaatggaga gtaaccttgc ctcaccctat taataatgct gaggactgcg aagataatcc
     3901 tgaggcctat caaaccacct actatcataa taaatatttt gatgtaccac agccagttgg
     3961 ttatgttaaa ccaactccaa aggttttgcc acttgtccag agcgagcaaa tctttctcgt
     4021 tttgctcttg ttgattttgg gatacttcga gcaaggtgta tatcgtattc gtatagttgc
     4081 tgatttctct ttcccactgc atccacgtca tgttatccca tatgtcattg tatgtcttgt
     4141 tgctccagga actattccaa ggaactgctg tagtacagat cagtttgcct gaacatcccc
     4201 atattcccaa cagctgctga tcttgcaagt atctctccag tgcgagtacg cgcgcctgca
     4261 gttgcttaat cccccagacc gtaagctgca acatgtgttg ttgcgcctct atggcgcgca
     4321 gcaagttact ctgttgttgg acgataccgg acaacaactg ccgagcttga actgtcaggg
     4381 tcatagacgc agcccccatg gttgaaccag cagctccgag aaatcccaga aacagcgctc
     4441 ccattccgac ggctcttttc tctctctcaa caactctccg cttcgccttg gtcggagcta
     4501 tgccaagtgg cttaatttcc actactttat acttgtacaa ttcgctcctc caattgtctc
     4561 gcatgtcccc tcctcccggt ctaaaagtct catttgttcc gttattcccc ccatctcttg
     4621 tgaggagaat accggtgata ttagatatac aggttatgtt cccagcgata ggaggggcat
     4681 acatagctct gccgactccc tgccacatat tgattatttg cttaattctg cacggcaaag
     4741 tgatgttact actgttggta taagtcgtat tttcaaaaag gtgcgtagtg ttacaataaa
     4801 aaaactctcc gcgacagttg aaactgtgct gcgtcacttc aagatcaccg ccagaatgcc
     4861 tcttaaattc aatagttttg ttggggaagt gctcctccag tttttttcgt actctgtcca
     4921 gtgttttatt ccagtcctcc ctgctgatgt tgcaataggc ttgcctgatg tctcctatga
     4981 tgtctgttgc aaagaatgtc tgtcccggcc ctatcctgat gctcttccgg gtgttattgt
     5041 ttgggcgaat acacactatt ttcacgcttt cgttaagatg aacgataatt gttttaccgt
     5101 tatcggtcaa attctcggac cttatgataa tttctccctc agcaagagaa ccgttgagga
     5161 gcaattgtgt agagaccact ggttttatgc catgggtaca ctgtactgtg ctgacgtttg
     5221 tgcaagggcc gaggccatta aaggtcttgt tgttgcattt aagaatagca tacccggcgg
     5281 gcgcgcagta gtgtatgggg atgggttcaa aagagacctt ggggcaggcc tgggtgattg
     5341 tggatgtatt gcaggagatc agcctgtact cagatgaatt agaattagga ttgagttgga
     5401 cgatgtccaa gttgtaaaag agcgctctcc ctttctgctt tttatctccc aaatctgctg
     5461 ttgtgttgaa ggtgcaattt ttcatggtgg atattgtgtc attggtattt tgagtggcat
     5521 tgacattttg gcaatccaat gtaacgcaaa gtggggtaag tttcacgcag ggcttaaggg
     5581 actgatccca caagctaatt atatcctcgt gcatctggtc gaccatatca ttcttccaca
     5641 tgttgaagtt ttccgtaaca ttttccatcc gcatctcttg ggggttaggg tctgtcggca
     5701 cgcatgcatg agttgcccat acattgtgaa cttcctgttc atatgctttg gcatcgctcg
     5761 cacagaagag tgtagttttt gcctctttcc acactggaac gccgtaatac actgtaaccc
     5821 acaagttcgc caatactgag gcaaccagca tgccgagcag gtacagcgtg gccaggggct
     5881 gcaaagaacc cataggcatg gtggcgctag cagctggtac ccagcttcta gagatctgac
     5941 ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
     6001 gcgtcaacgg ggcggggtta ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     6061 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     6121 atccacgccc attggtgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     6181 cgtagatgta ctgccaagta ggaaagtccc gtaaggtcat gtactgggca taatgccagg
     6241 cgggccattt accgtcattg acgtcaatag ggggcggact tggcatatga tacacttgat
     6301 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc
     6361 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     6421 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     6481 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
     6541 atggcggtca tattggacat gagccaatat aaatgtacat attatgatat agatacaacg
     6601 tatgcaatgg ccaatagcca atattgattt atgctatata accaatgact aatatggcta
     6661 attgccaata ttgattcaat gtatagatct tccataccta ccagttctgc gcctgcagca
     6721 atggcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac
     6781 tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc
     6841 acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa
     6901 accatgggtc tgtctgctca gtccagtcgt ggacctgacc ccacgcaacg cccaaaataa
     6961 taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc
     7021 agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     7081 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     7141 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     7201 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     7261 gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccgatccgg acgagtgctg
     7321 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     7381 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     7441 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     7501 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
     7561 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     7621 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     7681 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     7741 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
     7801 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
     7861 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
     7921 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
     7981 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
     8041 cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc
     8101 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
     8161 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
     8221 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
     8281 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct
     8341 gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg tcgctcggtg ttcgaggcca
     8401 cacgcgtcac cttaatatgc gaagtggacc tcggaccgcg ccgccccgac tgcatctgcg
     8461 tgttcgaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga
     8521 catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga
     8581 tgaagcaggg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
     8641 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
     8701 catctccagc agccgcacgc ggcgcatctc ggggccgacg cgctgggcta cgtcttgctg
     8761 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     8821 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     8881 cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8941 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9001 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9061 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9121 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9181 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9241 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9301 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9361 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9421 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9481 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9541 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9601 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9661 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9721 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9781 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9841 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9901 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9961 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
    10021 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10081 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10141 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10201 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10261 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
    10321 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10381 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10441 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10501 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10561 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10621 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10681 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10741 acgaggccct ttcgtcttca agaattctca tgtttgacag cttatcatcg ataagctgat
    10801 cctcacaggc cgcacccagc ttttcttccg ttgccccagt agcatctctg tctggtgacc
    10861 ttgaagagga agaggagggg tcccgagaat ccccatccct accgtccagc aaaaaggggg
    10921 acgaggaatt tgaggcctgg cttgaggctc aggacgcaaa tcttgaggat gttcagcggg
    10981 agttttccgg gctgcgagta attggtgatg aggacgagga tggttcggag gatggggaat
    11041 tttcagacct ggatctgtct gacagcgacc atgaagggga tgagggtggg ggggctgttg
    11101 gagggggcag gagtctgcac tccctgtatt cactgagcgt cgtctaataa agatgtctat
    11161 tgatctcttt tagtgtgaat catgtctgac gaggggccag gtacaggacc tggaaatggc
    11221 ctaggagaga agggagacac atctggacca gaaggctccg gcggcagtgg acctcaaaga
    11281 agagggggtg ataaccatgg acgaggacgg ggaagaggac gaggacgagg aggcggaaga
    11341 ccaggagccc cgggcggctc aggatcaggg ccaagacata gagatggtgt ccggagaccc
    11401 caaaaacgtc caagttgcat tggctgcaaa gggacccacg gtggaacagg agcaggagca
    11461 ggagcgggag gggcagg
//
